The Use of Gingipain DNA Vaccine Against Virulence of Porphyromonas Gingivalis : A Literature Review

Background: Porphyromonas gingivalis (P. gingivalis) is a primary etiologic agent of generalized aggressive and chronic forms of periodontitis. It releases toxins called ‘gingipains’.


Introduction
Periodontium surrounds the root part of teeth and is composed of gingiva, periodontal ligament, cementum and alveolar bone.Periodontitis is an infectious disease afflicting the tissues anchoring the teeth, eventually leading to loss of teeth [1,2].Data evaluated by the NHANES 2009-10 estimated that over 47% of the US population, representing 64.7 million adults has periodontitis [3].The literature suggests that periodontitis is linked with a mosaic of numerous microbiota of the oral biofilm.This biofilm induces local as well as systemic inflammatory responses, thus causing periodontal degradation.It has been decades since the recognition of the oral microbial diversity in humans, and today over 800 species have been identified [4,5].
Studies have reported that only 10-20 bacterial pathogens present in the dental plaque called the periodontopathic microbiota, lead to causation of destructive periodontitis.These include Porphyromonas gingivalis (P.gingivalis), Treponema denticola (T.denticola), Tannerella forsythia (T.forsythia) and Aggregatibacter actinomycetemcomitans (Aa) [6][7][8].Socransky et al. stated that periodontopathogens can be segregated on the color-coded system reflecting cluster analysis, community ordination and associated disease severity.Amongst these groups, 'red complex' including three species i.e.P. gingivalis, T. forsythia and T. denticola are considered the most pivotal in causing periodontitis.Among the red complex, P. gingivalis is the most effortless to grow and genetically manipulative, so it became the most investigated and examined periodontal bacterium [9,10].
Porphyromonas gingivalis, Gingipain, and Periodontitis P. gingivalis, under the environmental conditions that prevail during the host inflammatory response, is able to out-compete other bacteria present in the subgingival microflora and reach important levels within the community [2].It is a black-pigmented gramnegative anaerobic periodontopathogen.A varied number of virulence factors have been recognized behind the pathogenicity of P. gingivalis, namely fimbriae, lipopolysaccharides, various proteases and hemagglutinins [2].Ogawa and Hamada were the first to report the hemagglutinating activity and erythrocyte agglutinating activity of P. gingivalis.i.e. 381 FimA fimbria and their oligopeptide segments [11].
Gingipains are highly virulent cysteine proteinases secreted by P. gingivalis causing progression of periodontitis.Among other functions, they degrade inflammatory cytokines thereby down-regulating the host response and reducing inflammation.Gingipains

DNA Vaccines and Periodontitis
A number of techniques are being applied for development of vaccines to prevent periodontitis; in 1992 Evans et al. concluded that fimbriae from P. gingivalis are cardinal virulent factors which can be used as a target antigen and an effective vaccine against periodontitis [21].Use of the whole cell has also been quoted; in this, the entire cell was inoculated into the host to bring about immunization [22].In 2011 Kudyar stated use of outer membrane and observed that its use decreased activities of collagenase, gelatinase, and cysteine protease in gingival tissue [22].Another study also proposed the use of outer membrane vesicles of P. gingivalis as a mucosal immunogen and adjuvant for a periodontitis vaccine.It was observed that there was inhibition of gingipain proteolytic activity along with a decrease in viability of P. gingivalis [23].Wilensky et al tested recombinant and native HRgpA vaccination against P. gingivalis infection.Following immunization, the immune response was shifted towards anti-inflammatory response and there was a reduction in bone loss [24].
DNA vaccines have emerged as a favorable alternative to outmoded protein-based vaccines inducing immune responses.They are safe and stable to use, are specific in the induction of immune response to the antigen of interest including both B and T-cell responses, easy to manufacture and targets a wide variety of infectious diseases.The disadvantages include poor immunogenicity in clinical trials as naked plasmid DNA is often weak and is subjected to degradation by nucleases and demonstrate inefficient delivery to immune cells [25,26].In a study by Yonezawa et al., mice were immunized with rgpA DNA vaccine, controls were non-immunized mice and mice immunized with control plasmid pVAX1 (2.5 lg/mice) by Gene Gun.It was observed that there was a change in cytokine levels.The production of interleukin (IL)-2, 4, 5 and 12 was found to be elevated, whereas, interferon (IFN)-γ was lower in the serum of the DNA vaccine-immunized mice than in the serum of the control mice.This lowering of IFN-γ levels plays a major role in the protection of mice against bacterial attack.It was concluded that the rgpA DNA vaccine may induce humoral and cellular immune responses to bring about protection against P. gingivalis challenge [27].In 1998 Genco et al. carried out a study to evaluate the effect of immunization with gingipains on the ability of P. gingivalis of colonization and invasion in mice.Mice were immunized with the 95-kDa gingipain R1, the 50-kDa gingipain R2, or multiple antigenic peptides (MAP)conjugated gingipain R-derived peptides and then were subjected to challenge with P. gingivalis.It was observed that antibodies directed towards the amino-terminal region of the catalytic domain of gingipain R had capability to induce a protective immune response against P. gingivalis infection [28].are also involved in adherence and colonization of epithelial cells, vascular permeability, haemagglutination and hemolysis of erythrocytes by activating kallikrein/kinin pathway and the blood coagulation system, and it also plays an important role in degradation of host proteins and tissues [12,13].
The literature suggests that the virulence of P. gingivalis decreases in Rgp-and/or Kgp-deficient mutants [19].Rgp is responsible for producing surface proteins on P. gingivalis enlisting fimbrilin a 75 kDa protein, and prolyl tripeptidyl peptidase [18].HRgpA and RgpB cause generation of bradykinin by activating prekallikrein, whereas Kgp generates kinin directly from its precursor [20].Gingipains are even pivotal in maintaining a sustainable environment for bacteria to proliferate in the periodontal pocket [15].
Peptidases are a pivotal part in the causation of infection caused by P. gingivalis.Most of the ones listed are cysteine peptidases including lysine-specific cysteine protease i.e. lys gingipains (Kgp) and arginine-specific cysteine protease i.e. arg-gingipains A and B (HRgpA and RgpB).These two are the major virulence factors of this pathogen.Gingipains are cell-surface-anchored or soluble peptidases which represent around 85% of the total extracellular proteolytic activity of P. gingivalis [14][15][16].There are two genes called rgpA and rgpB encoding HRgpA and RgpB whereas only one gene kgp encodes lysine-specific gingipain K (Kgp) [11][12][13][14][15][16][17].rgpA and rgpB are similar in structure, except for the fact that rgpB does not contain the C-terminal adhesin domains of rgpA.There are three main components of rgpA and kgp which include a propeptide domain, a catalytic domain, and a C-terminal adhesion domain [11,18].The adhesion domain contains four subdomains enlisting Hgp (15, 17, 27 and 44) [16].hagA and tla encode the parts of the hemagglutinin domains of the kgp and rgpA genes [17].It is also observed that the adhesion subdomains are highly homologous between rgpA and kgp.In comparison to rgpA, rgpB consists of a propeptide domain and a catalytic domain which does not contain an adhesion domain [19].
In another study by Kuboniwa et al. the role of the catalytic domain of Kgp (Kgpcd) in hemoglobin binding by P. gingivalis was evaluated.To enhance antibody responses, a signal sequences was engineered into the plasmid vector which provided antigen secretion.The constructed DNA vaccines were even used in a genetic immunization strategy against P. gingivalis virulence to examine the effectiveness of Kgpcd as an immunogen.It was observed that generated antibodies were able to inhibit Lys-and Arg-specific proteolytic activities.The study showed that supplemented hemoglobin was the only iron source for growth in the iron-restricted condition.The Kgpcd acted as a hemoglobin-binding protein of P. gingivalis.It can be summarized that Kgpcd acts as a possible immunogen to induce a protective response to P. gingivalis infection [29].Yonezawa et al. in 2001 demonstrated that in order to induce a protective immune response against the periodontopathogen P. gingivalis, authors manufactured an rgpA DNA vaccine.In the process, they immunized mice intradermally with DNA carrying rgpA using Gene Gun.The vaccine was able to induce high levels of serum antibodies against P. gingivalis.It was also observed that sera from the immunized mice had an almost negligible proteolytic activity of Rgp A & B and binding action of P. gingivalis Journal Of Cell Biology And Histology to a type I collagen was also reduced to a minimum.Moreover, sera were effective in decreasing hemagglutination induced by P. gingivalis.The results suggested that the vaccine was capable of reducing the virulence of P. gingivalis [30].
Thus, it can be concluded that DNA vaccines are definitely effective in reducing the virulence of P. gingivalis.It can be used as an effective vaccine to prevent or reduce pathogenicity during the periodontal inflammation.
The authors would like to thank Mrs. Shanti Jha and Mrs. Parul Jain.This was a self-funded research.
In 2011, Muramatsu et al. also evaluated the protective effect of HRgpA DNA vaccine against virulence of gingipains on bone loss.They divided 6 weeks old female BALB/c mice into 8 groups including a non-immunized group and groups receiving vaccines containing the rgpA catalytic domain [pcat], the HGP44 domain-coding region (phgp44), the HGP 15-27 domain-coding region (phgp15-27), or the N-terminal (phgp44H] or C-terminal (phgp44T) half of the HGP44 domain-coding region against infection by P. gingivalis.There was a significant elevation of specific IgGs against P. gingivalis.This was highest in the rgpA DNA vaccine and in those immunized with phgp44.The reduction in loss of bone was observed to be most in rgpA DNA group followed by phgp44 group [32].The advantages that DNA vaccines include are that they are simple, easy to manufacture and stable by nature [22].Stimulation of adaptive and innate immunity reduction of lethality protects bacterial binding to hemoglobin; vaccine protein binds to hemoglobin and induces protective response and induce specific antibodies against enzymes avoidance of safety issues, unlike live vaccines, they do not revert back into virulent forms are other benefits [29,30,33].Along with this, there are few problems also related to DNA vaccine, such as limited potency in the clinical setting [34,35].
Miyachi et al. used rgpA DNA vaccine to immunize mice using a Gene Gun or intranasal route.The immunization was followed by injecting a specified quantity of P. gingivalis.Immunization evoked IgG and sIgA responses against P. gingivalis.There was a decreased loss of alveolar bone in both the groups but the effect was more prominent in the intranasal immunization group than in the Gene Gun group.Thus, the results suggested that immunization with the rgpA DNA vaccine through the nasal cavity is a competent method for the prevention of alveolar bone loss incited by infection with P. gingivalis [31].