A One-Year Hospital Based Prospective Study of Sickle Cell Disease from One Capital Area of Kuwait by HPLC

Volume 2 | Issue 2 Introduction A One-Year Hospital Based Prospective Study of Sickle Cell Disease from One Capital Area of Kuwait by HPLC AlDallal S* and Abdulsalam S Amiri Hospital, Kuwait *Corresponding author: Dr. Salma AlDallal, 25, Kuwait City, Kuwait, Amiri Hospital, 150300, Tel: +96590981981, E-mail: dr.s.aldallal@outlook.com Citation: AlDallal S, Abdulsalam S (2016) A One-Year Hospital Based Prospective Study of Sickle Cell Disease from One Capital Area of Kuwait by HPLC. J Hematol Blood Disord 2(2): 203. doi: 10.15744/24557641.2.203

The primary pathophysiology is based on the polymerization of deoxyHbS [13].A reduction in pH enhances HbS polymerization, as does a rise in temperature [14].The concentration of HbS in the red blood cells is also of great importance, with higher concentrations of HbS leading to more rapid polymerization.Another determinant of the tendency for polymerization is the presence of other haemoglobins, with HbA2 and HbF limiting the HbS polymerization to a greater extent than HbA and HbC.Homozygous patients have no HbA, an HbS% greater than 85%, an elevated HbF% and a normal HbA 2 %.In people with HbAS, the HbS% is approximately 40%.In patients with HbSC, the HbS% is about 10 to 15% higher and the mean corpuscular haemoglobin concentration (MCHC) is elevated, explaining why people with HbSC cab be severely affected [15,16].
Over the last few decades, there have been a number of studies indicating that anaemia is a significant public health problem in the State of Kuwait [17].A comprehensive electrophoretic screening of the Kuwaiti population was conducted in a tertiary hospital in Kuwait and showed that 6% of patients were heterozygous for HbS, while 0.9% was homozygous [18].

Materials and Methods
This retrospective study was carried out in the Hematology Laboratory Department, Amiri Hospital, Kuwait, for a one year period (2015).One forty-four sickle cell disease patients from adults and pediatric groups that presented with clinical anemia were referred for HPLC analysis.Records were also taken from 34 control patients.Medical history was retrieved from the hospital's medical records department, and each patient was examined by a haematologist.Data collected from patients records included age, gender, sickle solubility test, and percentage of haemoglobin fraction.

Results
As illustrated in Table 1, a total of 178 subjects participated in the study; 144 patients and 34 controls, with approximately equal numbers of men and women in both groups.The mean age for participants was 28.2 (SD 20.16).
Numerous techniques are used to screen for sickle cell trait or SCD, such as high-performance liquid chromatography (HPLC), haemoglobin electrophoresis, sickle solubility test, capillary electrophoresis, globin chain electrophoresis and DNA sequencing [19,20].However, it is essential to emphasise that in conjunction with any of these techniques, evaluation of the peripheral blood smear, as well as correlation with the results of a complete blood count (CBC) are crucial since many of the clinically significant haemoglobin disorders show distinctive peripheral blood findings, and are often co-inherited.HPLC has replaced alkaline electrophoresis as the main screening method of haemoglobinopathies.The consistency and reproducibility of results has made HPLC the method of choice for quantifying HbA 2 and HbF for laboratory identification of haemoglobinopathies [21].
The aim of the present study was to determine fractions of haemoglobins that appear in a chromatogram and to measure levels of Hb A, Hb F and Hb A 2 among SCD patients in one capital area during a one year period.

Study Design
In this study, target group adopted is, anaemic patients referred for HPLC analysis (HPLC-Waters; U.A.E) attending Amiri Hospital, Kuwait for a one year period (2015).Records of one-forty four sickle cell disease patients from adults and paediatric groups were retrieved from hospital data base for patients who diagnosed or suspecting to have a sickle cell haemoglobinopathies and confirmed by positive sickling test.Records were also taken from 34 control patients with healthy hospital records.
Ethical clearance was given by the Research Committee in the Kuwait Ministry of Health.

Ethical Issues
Statistical analysis was performed by Statistical Package for Social Science Software (SPSS) version 19 software.A Chi Square test was conducted to assess distribution of categorical variables (e.g.gender, age group, study group), and a Student's t-test was used in order to compare means of scale variable between groups.Statistical significance was set at P ≤ 0.05.The frequency of each phenotype within the study group showed, 110 participants (76.3%) having haemoglobin phenotype HbAS, and 34 (23.6%)having haemoglobin phenotype HbSS.

Frequency of haemoglobin phenotype (HbAS, HbSS) among patient groups
There was a significantly lower mean % of HbA in HbAS patients in comparison with the control group, while HbA was not found in any patients in the HbSS group.There was no significant difference in the mean % of HbA 2 in either the HbAS or HbSS patient groups when compared with the control group (Table 2), or in the mean % of HbA 2 between HbAS and HbSS (p = 0.73).HbS and HbF phenotype were only found in the two patient groups.A highly significant difference was observed in the mean % of HbS between HbAS and HbSS (p<0.05No difference was observed in the distribution of phenotypes in patients according to gender (Table 3) or according to age (Table 3).However, female patients were more likely to have a higher mean % of HbA (p = 0.08) and men were more likely to have a higher mean % of HbA 2 (p = 0.03) and HbS (p = 0.04).Higher mean % of HbS was found in older patients, compared to younger patients (p = 0.10; Table 4).Sickle cell disease is a major contributor to mortality and morbidity across the Middle East [17].This retrospective study aimed to estimate the prevalence of haemoglobin patterns amongst patients with sickle cell disease (SCD) in Kuwait.As expected, HbAS was the most common phenotype amongst patients with SCD [18], and no differences were observed in the distribution of phenotypes between males and females or according to age.

Discussion
HbA is the predominant haemoglobin in healthy adults, with reports suggesting that it contributes to approximate 98% of the haemoglobin load.In line with this, and illustrating the validity of our data, we observed a mean percentage of 97.5% HbA in our control group.That HbS was not detected in any control samples demonstrates that our assays are not associated with false Volume 2 | Issue 2 Journal of Hematology and Blood Disorders positives, providing reassurance in the identification of SCD patients.Less than 1% HbF values detected in our control samples represent a lack of sensitivity in assays, or that the level of HbF in our study population is lower than in other previously studied population.
As per previous studies, HbF levels were markedly raised in HbAS patients and HbSS patients compared to controls [12].Although there were no significant differences in HbF levels amongst males and females, or according to age, HbF was raised in the youngest age group, as expected given the dominance effect of HbF in the first few months of newborn infants.In line with previous studies, there were no differences between patients and controls with respect to HbA 2 levels, Shu et al. suggested that coelution of HbS adducts, including glycated HbS. with HbA 2 also contributes to increased measured HbA 2 concentrations Whereas HbA levels were markedly reduced in HbAS patients compared to controls [22].
HbS was only found in the patient group, and was significantly higher in the HbSS group.However, in contrast to previous studies, we did observe differences in HbA, HbA 2 and HbS according to gender [23,24].The similarities in HbS levels between the HbSS and HbAS groups is a result of all HbSS patients (including those in exclusion criteria) receiving a blood transfusion.The number of patients with accent blood transfusion included in this study is 144.
Further community based studies should be conducted to determine whether these differences are unique to our hospital-based population or are reflective of the general Kuwaiti SCD population.The variation in the prevalence of haemoglobin phenotype patterns between patients and controls is reflective of previous studies, and underpins screening and diagnostic testing within our population.
There were some limitations to this study.Due to the retrospective nature of the study there was some level of missing data throughout the dataset.In addition, this was a hospital based study and therefore may not be fully representative of the general population.However, our sample number was sufficient to examine differences between the populations and to accurately determine the prevalence of Hb phenotype patterns amongst our population.
In summary, this study has described the distribution of Hb phenotypepatterns in a Kuwaiti SCD population.The knowledge of the prevalence Hb phenotypepatterns encountered within our region is important to ensure that the formulation of diagnostics, preventative strategies and therapeutic options is appropriate to the patients requiring care.HbA 2 does not differentiate between patients and controls; however, changes in levels of HbA, HbS, and HbF are important for diagnosis and treatment strategies.

IntroductionA
One-Year Hospital Based Prospective Study of Sickle Cell Disease from One Capital Area of Kuwait by HPLC AlDallal S * and Abdulsalam S Amiri Hospital, Kuwait HPLC is a rapid, sensitive and reliable test for determining the presence of Hb patterns phenotype within a sample.Journal of Hematology and Blood Disorders Volume 2 | Issue 2 Submit your next manuscript to Annex Publishers and benefit from: Submit your manuscript at http://www.annexpublishers.com/paper-submission.php→ Easy online submission process → Rapid peer review process → Open access: articles available free online → Online article availability soon after acceptance for Publication → Better discount on subsequent article submission → More accessibility of the articles to the readers/researchers within the field Received Date: May 15, 2016 Accepted Date: June 27, 2016 Published Date: June 29, 2016

Table 2 :
). Journal of Hematology and Blood Disorders Volume 2 | Issue 2 The mean level of haemoglobin A, A 2 , F and S level in HbAS, HbSS patients compared with control group

Table 3 :
Phenotype according to gender and age

Table 4 :
Haemoglobin pattern according to age