Field Evaluation and Use of a Non Commercial Peptide Enzyme Immunoassay for Human Immunodeficiency Virus Serotyping in Abidjan ( Ivory Coast )

The fight against this pandemic needs simultaneous use of effective preventive action and wider access to treatment. However, in the absence of vaccine, fighting activities focus firstly on prevention through awareness of infection status and secondly on a better therapeutic management of infected persons. Given its importance in struggling against AIDS, biological diagnosis of HIV infection is importance and is based on the detection of antibodies in adults and children over 18 months. The “conventional” strategy associates antibody screening by one or more enzyme-linked immunosorbent assays (ELISA), and a confirmation of positive results with Western blot. This strategy is not always feasible in developing countries for many reasons. Indeed, this strategy The PBEIA uses peptides representative of the immunodominant regions of the transmembrane glycoprotein and the surface glycoprotein (V3 loop) of both HIV-1 (gp41 and V3.1) and HIV-2 (gp36 and V3.2). The evaluation of this assay was carried out using: 510 serum samples including 200 from HIV-negative individuals and 310 from HIV-positive individuals (130 HIV-1,105 HIV-2 and 75 HIV with dual seroreactivity) based on results obtained and 145 other samples positive for HIV-2. Murex HIV-1.2.OTM, Vironostika HIV Uni-Form II plus OTM and western blot tests were used as reference gold standard assays.


ISSN: 2394-6512
The fight against this pandemic needs simultaneous use of effective preventive action and wider access to treatment.However, in the absence of vaccine, fighting activities focus firstly on prevention through awareness of infection status and secondly on a better therapeutic management of infected persons.Given its importance in struggling against AIDS, biological diagnosis of HIV infection is importance and is based on the detection of antibodies in adults and children over 18 months.The "conventional" strategy associates antibody screening by one or more enzyme-linked immunosorbent assays (ELISA), and a confirmation of positive results with Western blot.This strategy is not always feasible in developing countries for many reasons.Indeed, this strategy The PBEIA uses peptides representative of the immunodominant regions of the transmembrane glycoprotein and the surface glycoprotein (V3 loop) of both HIV-1 (gp41 and V3.1) and HIV-2 (gp36 and V3.2).The evaluation of this assay was carried out using: 510 serum samples including 200 from HIV-negative individuals and 310 from HIV-positive individuals (130 HIV-1,105 HIV-2 and 75 HIV with dual seroreactivity) based on results obtained and 145 other samples positive for HIV-2.Murex HIV-1.2.O TM , Vironostika HIV Uni-Form II plus O TM and western blot tests were used as reference gold standard assays.
In conclusion, the excellent performance of PBEIA associated with its low cost confirmed that this assay can be used routinely by reference laboratories.This technique is also useful to reclassify all samples that are identified as HIV dually reactive with discriminatory rapid tests in peripheral laboratories.
HIV infection has become a global epidemic and a major public health problem worldwide.In 2015, among a total of 36.7 million [34.0-39.8]people living with HIV (PLHIV), 6.5 million [5.3-7.8] were in Western and Central Africa [1].In West Africa, Ivory Coast displayed the highest prevalence rate which was 3.2% among subjects aged 15-49 [2].Journal of Immunology and Infectious Diseases requires appropriate laboratory equipment, qualified staff, and is characterized by a high cost.Thus, the World Health Organization (WHO) recommends to use simpler and cheaper alternative strategies such as rapid diagnostic tests (RDTs) for countries with limited resources [3].There is a proliferation of commercial HIV screening tools, but according to the WHO, the genetic diversity of HIV requires the evaluation of any screening test prior to use in a given area [4].In Ivory Coast, the predominant subtype of HIV-1 is the recombinant form CRF02_AG [5,6], but HIV-2 also circulates as in other countries of West Africa [7].Therefore, screening strategies must also include a discriminating test in order to determine the HIV serotype in patients, mainly because of the natural resistance of HIV-2 to non-nucleoside inhibitors of reverse transcriptase [8,9].
The objectives of this study were to transfer and evaluate the performance of this tool in Ivory Coast and to describe its routine use during 8 years in our laboratory.
RDTs able to discriminate between HIV-1-or HIV-2-seroreactivity have been developed.Although several studies have described the good performance of tests and algorithms used in West Africa for HIV diagnosis the limited performances of discriminating rapid tests were noted [10][11][12][13].A large-scale implementation of more efficient commercial assays is challenging in countries with limited resources.
During the past two decades, non-commercial ELISA's assays have been developed for HIV serotyping dedicated to epidemiological studies conducted in developed countries.For instance, Barin, et al.. used a non-commercial ELISA based on peptides representative of immunodominant regions of the HIV glycoproteins (peptide-based enzyme immunoassay, [PBEIA]) to achieve the monitoring of HIV strains circulating in France [14][15][16].

Assessment Panels
PBEIA evaluation was conducted from June 2004 to November 2005 on two different sera panels from the biobank of the Centre for Diagnostic and Research for AIDS and Other Infectious Diseases (CeDReS) located at the teaching hospital of Treichville in Abidjan, Ivory Coast.
The ANRS 1201/1202 DITRAME PLUS study (for panel 1) and rapid test evaluation (for panel 2) were approved by the Ivorian ministry of health ethical committee and all participants gave their consent for HIV testing.

Description of the PBEIA
PBEIA is an indirect enzyme-linked immunosorbent assay adapted from that described by Barin and colleagues [15].PBEIA used for HIV-1 synthetic peptides of the immunodominant region of gp41 (consensus sequence of all clades and consensus of clade D respectively, RVLAVERYLKDQQLLGIWGCSGKLICTTAV and RILAVESTLKDQQLLGIWGCSGRHICTTAV) and the V3 loop (named V3.1).For HIV-2, PBEIA used synthetic peptides of the immunodominant region of gp 36 (RVTAIEKYLKDQAQLNSWGCAFRQVGHTTV) and the V3 loop (V3.2, respectively).These peptides were produced by PolyPeptide Laboratories (France) and used as antigens of the solid phase to coat FALCON flexible plates (BD Biosciences.Belgium).The coating was made in our laboratory with these antigens at the concentration of 1 µg/ml (gp41 and gp36) or 2.5 µg/ ml (V3.1 and V3.2) with overnight incubation at 37 °C.Then, after 3 washes with 150 µl of wash buffer (phosphate buffered saline with 0.5% Tween 20), wells are saturated with 200 µl of blocking buffer (phosphate buffered saline containing 2% fetal calf serum) (Sigma-Aldrich.USA) at 37 °C for 45 minutes, followed by three washes as above.After drying, plates are ready for use or can be wrapped in aluminum foil and stored at -20 °C for 6 weeks.
For serotyping, a 96-well plate is brought back to room temperature before use.Each sample and controls to be tested are diluted to 1/100 in a buffer (phosphate buffered saline containing 10% fetal calf serum and 0.005% tween 20).Only the samples retested were diluted to 1/500.One hundred microliters of each diluted sample are added to 4 wells of the plate allowing incubation with the 4 peptides (gp41, gp36, V3.1 and V3.2).In each plate, 3 negative controls, one HIV-1 positive control and one HIV-2 positive control are included.Thirty minutes after incubation at 18 °C, wells are subjected to 5 washes with 150 µl wash buffer each.
For panel 2, samples were screened with Vironostika HIV Uni-form II plus O TM assay and serotyped with New lav blot I and New lav blot II TM .In all cases, Western blot tests were interpreted according to the WHO criteria [4,18].

Reference tests
After its evaluation, PBEIA was used routinely at CeDReS between 2006 and 2013 for serotyping through the following algorithm.All samples were tested with Vironostika HIV Uni-Form II Ag/Ab TM for screening.Positive samples were serotyped with the PBEIA.For negative results, samples were retested by both tests.In case of HIV dual reactivity by PBEIA, samples were retested at dilution 1/500.
Thereafter, 100µl of 1/5000 dilution of conjugate: (F ʹ(ab) 2) anti-human immunoglobulin G goat antibody coupled to peroxidase (Clinisciences.Strasbourg) are distributed in each well of the plate and incubated for 30 minutes at 18 °C.After 5 washes, revelation is done by adding 100 µl of Ortho-phenylenediamine hydrochloride Di-fast as substrate (Sigma-Aldrich.USA).After incubation in the dark for 15 minutes at 18 °C, reaction is stopped by adding 50 µl of 2N sulfuric acid per well.The optical density (OD) is measured at 490 nm with a reference filter at 630 nm within 30 minutes.
For screening, sensitivity and specificity were calculated by comparing the PBEIA to non-discriminatory tests i.e.Murex HIV-1.2.O TM (Abbott.Dartford.United Kingdom) or Vironostika HIV Uni-form II plus O TM .For serotyping, sensitivity of each peptide was calculated for its ability to identify the right serotype.Similarly, specificity of each peptide was calculated for its ability to identify the correct serotype.Kappa concordance value was also calculated [19].

Results
All these results allowed us to define stringent criteria for the interpretation of the PBEIA: (i) consider only transmembrane peptides gp41 (HIV-1) and gp36 (HIV-2); (ii) samples giving an OD greater than 2 are considered positive for each peptide; (iii) samples giving an OD between 1 and 2 for a peptide, as well as positive samples for both peptides at 1/100 dilution should be retested on the same plate at both dilutions 1/100 and 1/500; (iv) samples tested at dilution 1/500 must be considered as positive if the OD is greater than 1.
The discriminatory power of the PBEIA, in comparison with the Pepti-LAV 1/2 TM assay, was high, with a Kappa value equal to 0.94 (Table 2).Thus, all samples showing an OD of less than 1 were considered negative by PBEIA.Negative threshold value at dilution 1/500 was set at 0.5 by applying the same principle.We also determined the PTV by analyzing ODs of HIV serotype 1 samples on the one hand and those of HIV serotype 2 on the other hand.PTV was set to 2 at dilution 1/100 (Figures 1b and 1c).PTV was set to 1 for dilution 1/500.1.Using a threshold of 2, the sensitivity of gp36 peptide was 93.8% (95% CI 89.9-97.7 %).The 11 samples detected by gp41 which had OD values ranging between 1 and 2 at dilution 1/100 were retested at dilution1/500.At this dilution, all of them gave an OD less than 0.5 and therefore were declared negative with this peptide.However, the 9 samples detected by gp36 with an OD between 1 and 2 at dilution 1/100 and retested at dilution 1/500, gave an OD greater than 1 and then declared positive.In total, PBEIA showed 100% concordant results with Western Blot on panel 2.
Operationally, the PBEIA was convenient and had a lower cost price, when compared to Pepti-LAV-1/2 TM (Table 4).However, its implementation can only be performed in laboratories with an adapted technical platform with at least an ELISA system.
During the eight years of use, 15,734 samples were tested in first intention (at dilution 1/100) with 15,462 samples HIV-positive.272 (1.7%) were tested negative and 760 tested positive for both HIV-1 and HIV-2 (HIV dual).After retesting the 272 samples negative by PBEIA, 74 were finally found positive including 50 with an OD lower than 2 for both peptides.It is noteworthy that when the OD ratio between the two peptides was greater than 5, the serotype is determined by the peptide having the highest OD.After retesting at dilution 1/500, the 760 samples which were dual positive at dilution 1/100, we obtained the following results: 388 HIV-1, 37 HIV-2 and 335 dual positives.Table 5 show distribution of ODs obtained respectively with gp41 and gp36 at the two dilutions, according to the final serotype found.For the 388 samples which tested HIV-1 positive with gp 41 peptide, only 7.7% (30/388) displayed an OD less than 1 at dilution 1/500.Similarly for the 37 samples which tested positive for HIV-2, 8% (3/37) presented an OD less than 1 at dilution 1/500 with gp 36 peptide.Overall, 1.3% negative results were obtained and the distribution of serotypes was 92.9%.3.7% and 2.1% for HIV-1, HIV-2 and HIV-dual reactive respectively.Serological profiles of the eleven samples which gave discordant results (3.6% discrepancy rate) are presented in Table 3.Of these, concordant results between PBEIA and Western blot assay were found for eight samples (72.7% concordance rate).

Discussion
In countries with limited resources, monitoring and diagnosis of HIV infection are major Public Health challenges [20].Immunoassays including ELISA and RDTs are the most common and widespread diagnostic methods [21,22].More efficient tests for serotyping such as Pepti-LAV TM and Western blot are very expensive for countries with limited resources [11,23].Moreover, the production of some commercial discriminating ELISA formats by developed countries was stopped for lack of profitability.In this context, technical performance of a non-commercial ELISA (PBEIA) and its use for eight years were assessed.This study was conducted in West Africa with a local panel gathering all the conditions related to the variability of HIV strains in a given territory, Journal of Immunology and Infectious Diseases The performance analysis of each peptide revealed that synthetic peptides including gp41 and gp36 sequences were excellent candidates for the diagnosis of HIV infection.Barin et al has been reported that the discriminating power of the PBEIA could be slightly improved by combining immunodominant epitope of both gp41 and V3 [15].Nevertheless, we obtained a low sensitivity of V3.1 peptide of HIV-1 and the two peptides of HIV-2 (gp36 and V3.2).In our study, V3 loop had no interest in determining the serotype of positive samples which finally took into account only the results of transmembrane peptides gp41 and gp36.Recently, Tiwari et al. developed a new antigenic cocktail including the 32 amino-acids heterovalent and oligomeric peptides "V3-I" in combination with gp41, gp36 and rp24.This increased the sensitivity and the specificity of an indirect ELISA assay developed for detecting anti HIV-1 and HIV-2 antibodies, thereby eliminating non-specific cross-reactivity.Moreover, a cross-reaction was observed with gp36 for two HIV-1 positive samples [24,25].These cross-reactions are mitigated when the samples were diluted at 1/500 instead of 1/100.The low sensitivity (96.2 %) of gp36 was comparable to those reported with commercial discriminating ELISA kits whose production was discontinued [23,26,27].This difficulty in detecting HIV-2 could be due to low HIV-2 viral loads, a characteristic of the HIV-2 natural infection, or could be due to the lack of detection of some subtypes of HIV-2.Indeed, HIV-2 variants of subtypes B are common in Côte d'Ivoire and we could have missed amplification our molecular technique [28,29].The reduced sensitivity of the PBEIA may also be caused by improper selection of antigen and/or reduction of HIV-2 antibody levels in patients [30][31][32].Marcellino increased to 100% the sensitivity an HIV-2 ELISA by using two new recombinant proteins, rgp36 and rpc2-C3 from HIV-2 primary reference isolate ALI [33][34][35].These data were consistent with previous studies showing that gp36 recombinant proteins derived from HIV-2 strains of several laboratories are highly immunogenic [35][36][37].It appears that routine use of PBEIA in our context, could disregard these V3 peptides.Thus, a ready-to-use ELISA plate could allow testing 48 samples instead of 24, and therefore reducing the unit price of the test.
Performance analysis of gp41 and gp36 peptides confirms the good sensitivity and specificity observed with the PBEIA.These results were similar to those obtained by Simon F and colleagues who found 98% specificity for an indirect ELISA developed from synthetic peptides gp41/gp36 and V3 of 4 lentivirus lines [38].Although the sensitivity of PBEIA does not reach 100%, our data show that its good specificity makes it useful in a serial algorithm as second test for serotyping.The very good agreement between PBEIA and Pepti-LAV-1/2 TM shows that it is a good alternative to commercial assay.Many dual-reactive samples by western blot were not confirmed by PBEIA.These results were consistent with previous studies including that of Tchounga, et al. who reported poor discrimination of dually infected samples in cohort of HIV-2 infected patients from 3 West African countries (Ivory Coast.Mali.Burkina Faso) [11,12,39,40,41].The choice of serotyping techniques for HIV infection should take into account not only the technical performance but also the operational characteristics and the cost of the test.For a laboratory with ELISA system and qualified staff, the use of PBEIA is a good alternative.Indeed, PBEIA is suitable for large series of samples.In addition, PBEIA presents lower price cost (about 1 USD).While, Pepti-LAV 1/2 TM and NEW LAV BLOT TM were more expensive with 25 USD and 100 USD per test.However, the routine use for nearly a decade (since 2006) of PBEIA at our lab CeDReS can raise some challenges: (i) Have enough flexibility in a results interpretation despite existence of positive and negative threshold values.(ii) Necessity to have another discriminant assay as an alternative for samples tested negative with PBEIA but positive with non-discriminatory assays.(iii) Necessity to retest at both dilutions (1/100 and 1/500) all samples giving an HIV-dual result at dilution 1/100 in order to reduce cross-reactions.
The excellent performance associate with low price cost of PBEIA reinforced our routine use of this discriminating assay.It may be used in reference laboratories to retest all samples dually reactive with discriminating rapid tests in peripheral laboratories.It would reduce the rate of HIV dual and render the management of antiretroviral treatments more accurate.

Panel 2
was obtained from patients who were enrolled in three HIV care centers of Abidjan: department of pneumology/teaching hospital of Treichville, Abidjan Integrated Center for Bio-Clinical Research (CIRBA) and Center for Care, Research and Training (CePReF).This panel contained 145 samples positive for HIV-2, according to ELISA and WB results.Samples were collected during a study dedicated to an evaluation rapid test for the health ministry from January to march 2007.Sera of Panel 1 were collected from pregnant women attending in six urban community based antenatal clinics in the city of Abidjan during the ANRS 1201/1202 DITRAME PLUS project from March 2001 to April 2003.This was an open cohort conducted in Abidjan whose objective was to evaluate the efficacy of antiretroviral short regimens (AZT + NVP and AZT + 3TC + NVP) in preventing mother to child transmission of HIV (PMTCT) [17].This panel consisted of 510 samples including 200 negative and 310 positive (130 HIV-1, 105 HIV-2 and 75 HIV-1+2 dual reactivity), according to WB results.

Table 1 :
Detection performance of different HIV peptides

Table 3 :
Profile of the 11 discordant samples Journal of Immunology and Infectious Diseases Results obtained on panel 2 are shown in Table