Anti-Diabetic Activity of Lycopene Niosomes: Experimental Observation

The lycopene is a known bioactive constituent of Lycopersicum esculentum and its application in diabetes is an active area of research. The lycopene has disadvantages such as susceptible to light, heat, and oxidants, which limit its therapeutic applications. In the present investigation, we reported lycopene niosomes formulation which can enhance its anti-diabetic application. The niosome formulation was prepared to preserve lycopene activity. The niosomes were characterized by entrapment efficiency, particle size, drug release profile, zeta potential and stability studies. The anti-diabetic activity of the formulation showed a significant reduction in blood glucose level compared to diabetic control on seventh and 14th days (p<0.01 and p<0.001 respectively). The biochemical parameters such as total cholesterol, TGL, LDL, and VLDL were significantly decreased in treated groups when compared to control group. The overall results indicate that the lycopene-loaded niosomes are effective against diabetes. The niosome formulation is promising for wider applications which can play an important in the drug delivery and formulation research. Abstract ISSN: 2348-9782


Introduction
In the present investigation, we have formulated the lycopene niosomes. This study aimed to protect the anti-diabetic activity of lycopene in niosomes. The mechanism therein is to form a wall system to entrap the lycopene (core). For the wall system, span 60 was used as non-ionic surfactant and cholesterol which acts as membrane stabilizer to assist in solute retention by forming bilayer

Isolation of lycopene
The lycopene was isolated from tomatoes (Lycopersicum esculentum), as described in the method by Sharma, et al. 2016. The resulting extract was stored in an amber color glass container with the inert environment at 4 °C for further processing. The extract was dried using rotary flash evaporator in inert environment of nitrogen gas by heating at 60 °C at 50 rpm in dark condition [26].

Preparation of lycopene formulation
The lycopene niosomes were prepared by the method described by Sharma, et al. 2016. In this method, the pure extract was collected and dispersed in a small amount of solvent system (n-hexane: acetone: ethanol in 2:1:1 v/v). About 22 ml of span 60 and cholesterol (1:1mol) were added to10 ml of above solvent system as organic phase having wall material. This phase was adsorbed over inert media with constant stirring at 60 °C temperature, as described by Sharma, et al. 2016. The aqueous phase (10 ml, mannitol solution: pH 7.4) was then slowly added to the lycopene-loaded inert media with vigorous shaking at 60 °C temperature followed by sonication using an ultrasonic cell crusher (classic, sonicator, Lark Innovative Fine Teknowledge, Chennai, India) for 60 seconds/cycle in three cycles to protect the solution from heat buildup. Then it was kept for 2-3 hours for niosomes to stabilize. The suspension of self-assembled, lycopene-loaded niosomes was filtered using 0.45-mm whatman filters (No-1) to remove inert material. The filtered suspension was then passed through column chromatography (Sephadex G-50 column) to separate un-entrapped lycopene. Finally, the niosome vesicles were collected and lyophilized (Lyodel, Delvac, India). The lyophilized formulation was stored in an air tight high-density plastic container [26].

Characterization
The pure lycopene extract was characterized, followed by lycopene niosomes characterization in terms of particle size, entrapment efficiency, zeta potential and in vitro drug release. Its biological activity was characterized by in vivo study in diabetic rats.

Characterization of lycopene:
The characterization of lycopene was studied as described by Sharma, et al. 2016. They were characterized by physical appearance and microscopic characteristics, melting point (using Barnstead Mel-Temp Electrothermal Model 1001), solubility, determination of absorption maxima (λ max ) using UV spectrophotometer (Shimadzu 1700) and the absorption spectrum (λ max ) was obtained. The standard curve of the lycopene in n-hexane was prepared at the observed absorption maxima (O'Neil, 2006). The regressed calibration curve was plotted and observed for linearity and along with concentration range for beer lamberts law [26].

Characterization of lycopene-loaded niosomes:
Particle size analysis and zeta potential determination: Lycopene niosomes were spray dried on a clean glass slide (1x1 cm) and placed in a sample holder and fixed. Copper tape is used to mount sample to the stub. It was then gold coated. It was observed using JSM-6390 Scanning Electron Microscope (JEOL, USA) for evaluation of their morphology, particle size, and particle size distribution. Zeta potential of vesicles is determined by using zeta sizer (Malvern Instruments, UK). Niosomes suspension was diluted with distilled water and dropped into the zeta sizer electrophoretic cell. Each sample was measured six times at 25±0.1 °C.

Entrapment efficiency (EE):
The lycopene niosomes were loaded onto a Sephadex G50 column and was eluted with distilled water. The lycopene-loaded niosomes were eluted while free lycopene remained bound to the column. These separated vesicles were disrupted through the addition of 0.05 ml TritonX-100 in 1 mL formulation in a centrifuge tube, and ultra-centrifuged at 120000 g for one h (Beckman L8-M, Beckman Coulter s.r.l., Milan Italy). The total lycopene-loaded in niosomes and the lycopene entrapped in niosomes were recovered and assayed by UV spectrophotometric method. The encapsulation efficiency was calculated using the following equation: Encapsulated drug present after chromatography % x1 00 Total drug present before chromatography EE = Stability studies: Stability study of lycopene niosomes and the unloaded niosomes was carried out at 4 °C and 25 °C, and in different pH conditions. Particle size, zeta potential and encapsulation efficiency were measured immediately after the preparation and on pre-determined time during the three-month storage [7,27].
In vitro drug release profile: The in vitro release of lycopene from niosomes was carried out using dialysis system. It consists of a dialysis bag and a receptor. The hydrated dialysis membrane (15 kD) was tied to the funnel of diffusion cell assembly, and the setup was constituted. The niosomes (3 ml) were packed in donor compartment (dialysis bag) closed by dialysis clip and placed in a beaker (receptor compartment) having 250 ml phosphate buffer saline (PBS) (pH 7.4). The PBS was stirred at 50 rpm (37±1 °C) using magnetic stirrer. The sampling was done by withdrawing 4 ml of sample periodically till 72 h and replaced by fresh PBS of equal volume. The withdrawn samples were analyzed for drug content using UV spectrophotometer at λ max (205 nm). PBS was used as blank [28,29].
Anti-diabetic activity of lycopene-loaded nano-niosomes Animals: Wistar rats (150 g) of either sex, approximately 6-8-week-old were procured from Institute of Animal Health and Veterinary Biologicals, Indore, India. The animals were maintained in polypropylene cages with 12 h light and dark cycles at a temperature of 23±2 °C and a humidity of 55±5%. They were maintained on laboratory diet and water ad libitum. The animals were acclimatized to laboratory condition for one week before the start of the experiment. The experiment was carried out following protocol approved by Institutional Animal Ethics Committee (TIT/IAEC/831/PHARMACEUTICS/2012/07) at TIT-Pharmacy, Bhopal, India.
Preparation of diabetic rats: The hyperglycemia was induced by intraperitoneal injection of a freshly prepared aqueous solution of alloxan monohydrate (SD Fine Chemicals Pvt. Ltd., Biosar) (150 mg/kg), to overnight fasted rats. The normal control rats received a similar volume of vehicle, normal saline (2 ml/kg body weight) along with diet. Animals that did not develop hyperglycemia after 48 h of alloxan injection were rejected, and new animals were used. Immediately after confirmation of diabetes, rats were classified into six groups of six rats each [30].

Experimental design for anti-diabetic activity:
This study involves six groups of rats which comprise of two control groups (normal and diabetic controls) and four test groups; each group had six rats and housed three animals in each cage, for proper identification. Group I-normal control: The animals in this group were given normal saline orally, and they were fed with regular rat chow and water ad libitum. Group II-diabetic control: The animals were injected intraperitoneally with alloxan monohydrate 150 mg/kg to induce diabetes mellitus. Also, the animals were fed with regular rat chow and water ad libitum. Group III & IV were treated with lycopene niosomes by oral administration (100 & 200 mg/kg respectively) along with normal rat chow and water ad libitum. Group V was treated with Glibenclamide (5mg/kg) with normal rat chow and water ad libitum, and Group VI received pure lycopene extract (200 mg/kg) orally and served as reference standard. The treatment continued for 14 consecutive days. The blood samples were collected from the tail vein of the animals, and the fasting blood glucose level was estimated on 0, 7 th and 14 th day of dosing period [31].

Biochemical analysis:
The blood was withdrawn on the 15th day directly from the heart through cardiac puncture method; the blood was collected in vacutainer tubes. The collected blood sample was centrifuged at 3500 rpm for 20 minutes, and serum glucose levels were determined using the glucose oxidase-peroxidase method (Coral Clinical Systems, Goa, India) [32]. The biochemical parameters such as serum triglyceride (ST), serum cholesterol (SC) and high-density lipoprotein (HDL), very lowdensity lipoprotein (VLDL) and low-density lipoprotein, (LDL) were determined [33].

Statistical analysis
The results are expressed as Mean ± Standard error (S.E). All the statistical analysis was performed with Graph Pad Instat Software (Version 3.0, Graph Pad Software, California, USA) using one-way analysis of variance (ANOVA) followed by Dunnett's 't' test for multiple comparisons and student's t-test for a single comparison. Significance was expressed as *p<0.01; **p<0.001 [34][35][36].

Isolation of lycopene
The isolated lycopene extract appeared as odorless brick red crystals as reported by Sharma, et al., 2016. The microscopy revealed the appearance of lycopene as long deep red needles. The melting point range was confirmed as 172-173 °C which complies with pure lycopene. The solubility of lycopene was done to confirm its solubility; the solubility was studied with various solvents, and it complies with literature [7,37,38]. The purity of lycopene was determined with UV spectrophotometric method using absorption spectra (λ max ); the λ max was obtained at 504 nm which is comparable with the reference spectrum of the lycopene [1]. The initial characterization was revealed the presence of pure lycopene in the extract.

Formulation development and characterization of niosomes
The isolated lycopene extract was formulated in niosomes and this lycopene niosomes formulation was characterized.

Results
Physical characterization (Particle size, size distribution and zeta potential): The particle size and size distribution of lycopene Volume 4 | Issue 1 niosomes were ranged from 175 to 235 nm with average hydrodynamic particle size 202±41 nm which is similar to results reported by Sharma, et al., 2016, owing to same methodology of preparation. Morphology of niosomes was studied and confirmed using scanning electron microscopy (SEM) which was found to be in accordance with hydrodynamic particle size. The particles were spherical and morphologically similar. The zeta potential was studied at different pH conditions (4, 5.6 and 9), zeta potential value was found to be stable at -2.25±0.2 mV and the changes in zeta potential over stability period was not significant, indicating the absence of ionization of the membrane components [14,16,18,39].
Stability studies: The stability of both empty and lycopene niosomes, were monitored in terms of vesicle size, EE, and zeta potential during three-month storage at 4 and 25 °C. The zeta potential of empty niosomes did not change in an important way at different pH conditions and during storage conditions.
The oral administration of the formulation in the doses 1000 to 5000 mg/kg did not produce significant changes in behavior or toxicity in the rats indicating that the formulation is not toxic under the observable conditions (OECD revised draft 420). Administration of lycopene nano-niosome formulation (100 & 200mg/kg) for 14 days has reduced the blood glucose level of diabetes induced animals as compared to diabetic control group. There was a significant decrease in the blood glucose level on the 7 th (p<0.01) and 14 th (p<0.001) day of the diabetes induction, indicating the anti-diabetic effect of the lycopene niosome formulation ( Table 1). The lycopene niosomes (100 mg/kg) were able to reduce the blood glucose level to 0.6 times compared to diabetics control after 7 days and 0.4 times after 14 days. Whereas, lycopene niosomes (200 mg/kg) reduced the blood glucose level to 0.4 and 0.35 times related to control after 7 days and 14 days respectively. The efficacy of lycopene niosomes were found were similar to pure lycopene extract as well as Glibenclamide (positive control), but lycopene niosomes (200 mg/ml) were more effective than pure lycopene extract. The niosomes were able to produces time dependent control over blood glucose level over 14 days. The control release profile will further enhance the effectiveness of the formulation for prolong period. This might me due to diffusion control lycopene delivery as supported by in vitro kinetics.

Drug release profile:
The drug release profile of lycopene niosomes was observed, and linear regression analysis of lycopene release data supports the diffusion controlled mechanism of its release. It was found that initially lycopene release was at higher rate which can be contributed to the membrane-bound lycopene, followed by encapsulated lycopene from the formulation. During first 24 h, the initial lycopene release was found to be about 19% and during the next 48 h, it was 48.3% of the total lycopene from niosomes. The mathematical models revealed and supported the mechanism of lycopene release from niosomes (Zeroorder kinetics, First-order kinetics, Higuchi kinetics, Korsmeyer-Peppas and Hixson-Crowel models). The sustained release of membrane bound lycopene and followed encapsulated lycopene supports the prolonged release function of the niosomes. Higuchi kinetics, Korsmeyer-Peppas and Hixson-Crowel models supported the mechanism of lycopene release. This implies that lycopene in the present formulation has a sustained and prolong release profile, which is required to maintains an optimum concentration for its in vivo activity, which is very important for different herbal and natural product applications. The lycopene niosomes also produced a significant decrease in the biochemical parameters viz., total cholesterol, TGL, LDL and VLDL when compared to diabetic control animals and increased the HDL levels indicating the protective effect of the lycopene formulation on diabetes-related complications, as given in Table 2. The TGL level for 200 mg/ml dose lycopene formulation were significantly less than diabetic control as well as pure lycopene extract, whereas the 100 mg/ml did not produce any significant decrease, which might be due to low dose of lycopene and high metabolism over 14 days. The HDL levels were significantly

Discussion
The niosomes are well-established vehicles for better pharmacokinetic and pharmacodynamic profile than other conventional formulation of natural extracts due to its higher affinity to the phospholipids. This improves the absorption of the active drug and molecules, and increases the duration of the activity as it slowly releases the active principle. The niosomes formulation is stable and has sustained releases profile which makes it a carrier of choice.
The isolated lycopene was formulated in niosomes as the lycopene degradation is caused during processing or formulation by isomerization and oxidation, due to the influence of factors like heat, light, and oxygen, against which it can act as a potential system. The niosome are processed under less stressful conditions and is suitable for lycopene as it can be process below degradation conditions. It can provide high resistance to oxidative stress induced by different sources of free radicals [26,40].
The lycopene niosomes prepared were successful and found to be in uniform size having high entrapment efficiency (EE), which can be attributed to the maximum surface area for contact achieved during development between wall material and lycopene. The span 60 for wall system was the best choice due to its potential properties of emulsification, water-solubility, film-formation, edibility and biodegradable property. It also helps in providing the maximum EE, due to its alkyl chain length and phase transition temperature. Also, cholesterol acted as membrane stabilizer to assist solute retention by forming the bilayer, thus providing higher entrapment efficiency and stability as well as it improves the adhesion force between the wall and core materials [26]. The mannitol solution (pH 7.4) was used as an aqueous phase to impart isotonicity to the niosomes. In niosome's structure, the lycopene was predicted to be embedded in the bilayer of surfactant and cholesterol through a hydrogen bond, thus was found to be protected against oxidative stress. The wall system of long chain surfactant and cholesterol help in protection against light. The absence of agglomeration and instability was observed in the niosomes formulation. The entrapment efficiency of the niosomes was 62.8±2% (n=3), and the average particle size of the niosomes was 202 nm with a uniform, spherical unilamellar outer structure. The particle size of lycopene niosomes was supportive in circumventing the bio-environment and avoids the uptake by rough endoplasmic reticulum. The zeta potential of empty niosomes has not shown any significant change at a different range of pH from 4 to 9, indicating the absence of ionization phenomena of the membrane components. Both empty and lycopene niosomes confirmed good stability over a three-month storage period. In fact, there was no statistically significant difference in size, zeta potential and entrapment efficiency of the niosomes (P>0.05) after three-months storage at ambient temperature. Moreover, the loss of entrapment efficiency of niosome formulation was not significant during stability studies. The in vitro lycopene release was gradual with time. During initial release phase of lycopene, it was faster over a period of 10 hours. This was perhaps due to the incorporation of lycopene in the outer wall of niosomes. The lycopene release after that was found to be steady, and 67.3% of the entrapped lycopene was traced during 72 h. The lycopene release mechanism from niosomes formulation was determined using mathematical kinetic models: Zero-order kinetics, First-order kinetics, Higuchi kinetics, Korsmeyer-Peppas and Hixson-Crowel models [26]. The mechanism of drug release was found to be the Fickian type and initially obeyed zero-order release kinetics for initial 10 h, after that the regression equation was the best fit in Korsmeyer-Peppas model (r 2 =0.9915). Thus, the in vitro lycopene release from niosomes was sustained, and prolonged profile required for effective treatment, which might be due to maximum lycopene entrapment efficiency and uniform sized particles prepared innovatively.
reduced as compared to diabetic controls. The efficiency of lycopene niosomes (at both 100 and 200 mg/ml dose) in lowering LDL levels were significantly high as compared to diabetic control and pure lycopene extract, whereas there was no much difference in VLDL levels. The total cholesterol was also significantly reduced by lycopene niosomes as well as pure lycopene as compared to diabetic control but effectiveness of lycopene niosomes were more pronounced than pure extract.
The antidiabetic study showed the marked decrease in blood glucose level as well as various biochemical parameters in diabeticinduced rats when treated with lycopene formulation of doses (100 & 200mg/kg) for 14 days as compared to antidiabetic drug glibenclamide (5 mg/kg). The level of blood glucose in lycopene treated group (91.6±4.3 mg/dl) reached almost equal to glibenclamide treated group (90.8±6.2mg/dl) after 14 days of treatment. Thus, provide evidence that the lycopene at a dose of 200 mg/kg is having antidiabetic activity similar to glibenclamide (5 mg/kg). The observed activity might be due to the preservation of potent activity of lycopene, which attenuates T cell-dependent adaptive immune response, probably by increasing total antioxidant capacity of the serum and also due to its effect on glucose metabolism and possibly insulin secretion. Lycopene is a powerful antioxidant with a singlet oxygen quenching capacity greater than that of β-carotene and vitamin E by 47 and 100 times, respectively [39,41]. This lycopene formulation also has a potent hypo-cholesterolemic activity as shown in results [42]. These results show the superiority of the method used for the preparation of lycopene niosome formulation in its stability, drug release and more importantly in preserving its in vivo and in vitro activity.
We would like to acknowledge management and staff of all the affiliated department of institutions of authors for their support during the study.
The lycopene niosome was found to be efficient and has well-preserved the lycopene's activity. The niosomes promises to be a potential technique for natural product development. The niosomes have potential in efficient delivery of a broad spectrum of anti-diabetic natural products and agents. The formulation is simple and reproducible for further applications, and could be useful for different applications.
Conclusion Acknowledgement