The BMBL and Biosafety Levels

Volume 1 | Issue 1 Scientists began developing and publishing a series of best practices to mitigate laboratory risks in the 1970’s. These biosafety guidelines are disseminated by the Department of Health and Human Services in the publication Biosafety in Microbiological and Biomedical Laboratories (BMBL). Now in its 5th edition, the BMBL publishes guidelines for appropriate laboratory biosafety and biosecurity.

Since the first edition was published, each edition of the BMBL has described primary and secondary barriers to infection as well as standard and special laboratory practices for each biosafety level [1,2].Primary barriers include equipment such as biosafety cabinets and safety centrifuge cups-each designed to prevent release of aerosols.Secondary barriers refer to the laboratory design itself, and include items such as an on-site autoclave and separation of the laboratory from public areas [1].The National Research Council's 2009 report-Responsible Research with Biological Select Agents and Toxins-states that the biosafety recommendations in the BMBL are almost universally followed as the standard precautions for both research and diagnostic laboratories [3].The containment biosafety level (i.e.BSL1 thru BSL4) needed for each submission/patient/case is determined by the combination of laboratory facilities (engineering controls); practices and procedures (administrative controls); and the safety equipment (personnel protective equipment) that will be utilized (Table 1).A typical high school science laboratory where individuals work with agents that are not expected to cause disease in healthy adults is a BSL-1 laboratory.In BSL-1 laboratories standard microbiological processes are followed (no eating or drinking in the lab; no mouth pipetting; everyone must wash hands before leaving the laboratory; etc.) and the facility is required to have a laboratory bench and sink.BSL-2, -3, and -4 laboratories (Table 1) are designed, constructed, and operated to "…prevent accidental release of infectious or hazardous agents within the laboratory and protect laboratory workers and the environment external to the laboratory, including the community, from exposure to the agents" [4].These guidelines are based on the combination of the procedures to be performed and the specific transmissibility features of the agent.Thus, "…the correct biosafety level depends on the pathogen, its potential for transmission and [availability of] treatment, and the research procedure or activity being [performed]" [1].This means that in some situations, otherwise deadly pathogens can be safely handled at less restrictive biosafety levels, depending on the activities being performed with that pathogen.For example, diagnostic testing to identify the presence of a pathogen may require BSL2 containment while amplifying (i.e.growing more of) the same pathogen in a research or production setting may require BSL3 containment [5].
Organisms are not known to routinely cause diseases in healthy adults.

BSL-1
Pathogens can be associated with human disease.Routes of infection include ingestion, contact with skin or mucous membranes (eyes, nose, and mouth) but NOT AIR-BORNE TRANSMISSION.Confine aerosols created by centrifugation, etc. inside biosafety cabinet if needed.

BSL-2
Agents that may have serious or lethal health implications for humans.Routes of infection MAY INCLUDE AIR BORNE TRANS-MISSION.Do all work in containment (i.e.inside a biosafety cabinet).Facility must provide negative airflow and alarms to detect changes in airflow.

BSL-3
Agents that are frequently fatal in humans and for which there are no vaccines or treatments.Route(s) of infection are UNKNOWN or include AERO-SOL TRANSMISSION.All work done inside an in-line Class III biosafety cabinet or the investigator must be wearing a positive pressure suit and using a Class II biosafety cabinet.Determining when BSL3Ag containment is required involves evaluating 1) the potentially severe detrimental impact on agricultural animal health or animal products; 2) the virulence and transmissibility of the agent; 3) the availability of an effective treatment method(s)for people and/or animals; and 4) the potential impact to the national and/or global economy.Thus, although not necessarily zoonotic, high consequence livestock pathogens require handling using BSL3 or BSL3-Ag containment practices and procedures to prevent accidental or intentional release of the pathogen into the environment [7].

BSL-4
Whether an animal is being evaluated by a diagnostician in the laboratory or a veterinarian in the field, the performance of a risk assessment prior to examining the animal is the same.Knowledge regarding typical clinical signs, host range, basic epidemiology, geographical distribution of diseases, and familiarity with zoonotic and high consequence pathogens is used to determine the safety requirements [7].Thus from farm to fork, veterinarians truly are instrumental in ensuring that the appropriate safety procedures are followed.
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Table 1 :
Laboratory Safety Levels

The BMBL and Biosafety Levels Tanya D. Graham *
Biosafety Consulting for Veterinary Medicine, LLC diseases.Worker safety is important but-with the exception of zoonotic diseases-"greater emphasis is placed on reducing the risk of the agent escaping into the environment" [4].The 2011 survey-Biosafety & Biosecurity in U.S. Veterinary Diagnostic Laboratories:Results of a National Survey by Laboratory Employees-indicates that most veterinary diagnostic labora- Graham T, Veterinary Laboratory Diagnostics in Biological Safety.Principals and Practices (5th edn).In press.toriesfollow the BMBL recommendations and the majority of laboratories operate under BSL2 containment conditions [6].