Streptomyces species are medically, industrially, and microbiologically important bacteria that are found in soils and other habitats. Streptomyces isolates and QUBC97 were cultured on a new medium (CCG). Agar plug diffusion test showed antibacterial activity. N-butanol extracts analysed by silica thin layer chromatography (TLC), HPLC purified and identified by MS analysis. QUBC97 was identified based on morphology, PCR, and DNA sequencing of two loci.