Articles Related to Anti oxidant
Assessment of Antioxidant and Antineoplastic Activities Blumea Lacera (Burn. F) Leaves
Blumea lacera (Burn. f.) DC. (Family: Asteraceae) is an important member of Bangladeshi natural plant resource and it is an herbaceous weed locally known as Kukursunga. Different parts of this plant are used traditionally to cure various diseases. But detail study on the antioxidant and antineoplastic potentials of Blumea lacera leaves, has not yet been done. Aim of this study is to examine the antioxidant and antineoplastic properties and quantify the different type of phytochemical content of the methanolic extract of leaves of Blumea lacera (MELB). MELB contained a rich polyphenol, flavonol, flavonoid and proanthocyanidins. MELB showed moderate cytotoxic effect against Artemia salina (brine shrimp nauplii) where its LD50 values was 66.12 μg/ml. In vitro antioxidant assay, MELB exhibited a remarkable capacity to scavenge the tested reactive species. MELB scavenged DPPH with an IC50 of 33.64 μg/mL and 42.69 μg/mL, respectively. In vivo antineoplastic assay, MELB significantly (P<0.05) decreased viable cell count and increased the survival time of EAC cell bearing mice. Hematological profiles were also restored significantly (P<0.05) to normal levels in MELB treated mice as compared to untreated EAC control mice. In addition, fluorescence microscopic view of EAC cells derived from MELB-treated group showed apoptotic characteristics in treated cells compared to untreated EAC control. our findings suggest that methanolic extract of Blumea lacera leaves(MELB) might be a potential agent with antioxidant properties for prevention of cancer and has the merit for further investigation in isolating its active constituents.
Anti-Inflammatory and Anti-Arthritic Efficacy and Safety of Purified Shilajit in Moderately Arthritic Dogs
The objective of this investigation was to evaluate the efficacy and safety of purified Shilajit in moderately arthritic dogs. Ten client-owned dogs in a randomized double-blinded study received either a placebo or Shilajit (500 mg) twice daily for a period of five months. Dogs were evaluated each month for physical condition (body weight, body temperature, heart rate, and respiration rate) and pain associated with arthritis (overall pain, pain from limb manipulation, and pain after physical exertion).