Articles Related to ELISA
Development and Validation of a Novel ELISA for the Detection of Neospora Caninum Antibodies in Bovine Sera
Neospora caninum is a world-wide distributed apicomplexan parasite, causative agent of bovine neosporosis, which is one of the major causes of reproductive losses in cattle affecting both dairy and beef industries. Several techniques are regularly used for the diagnosis of bovine neosporosis.
Hydatid disease is an important emerging neglected disease worldwide, with significant geographic variation in seroprevalance. The disease is commonly diagnosed on basis of clinical suspicion, imaging and serology. This study was conducted to evaluate our experience with serology in diagnosing hydatid disease in terms of identifying the numbers of patients testing positive for antibodies against Echinococcus, and to study their clinical profile.
Occurrences of Dairy Calf Mortality and Morbidity and the Associated Risk Factors in Sululta and its Environs, Central Ethiopia
Calf morbidity and mortality are important causes of economic losses on dairy farms worldwide. A cross-sectional study and clinical observation was conducted from November 2016 to April 2017 with the objective of determining calf morbidity and mortality and to investigate the potential risk factors for mortality and morbidity in Sululta and its environs. A total of 312 respondents engaged in market oriented small holder dairying were interviewed using a structured questionnaire survey about their farm and calf management practices and major calf health problems encountered and diseases that causes mortality. The overall magnitude of morbidity and mortality of calves were 31.0% and 58.37%, respectively. The major calf diseases found were diarrhea (69.34%), pneumonia (16.54%), liver fluke (2.4%), bloat (2.0%), joint ill (2.4%) and other cases (8.04%). Risk factors such as weaning age, breed and awareness of colostrums, feeding of calf and overall farm management were included. In this study 80.3% of calf mortality occurs under age 3 month and 19.42% is above 3 month. Based on laboratory examination, Salmonella and E.coli were detected from diarrheic calves. Salmonella found at rate of 2/29 (3.6%) and E.coli found at only genus level. In conclusion, the magnitude of calf morbidity and mortality found in this study were much higher than economically tolerable level and could greatly affect the productivity of the dairy farms through mainly decreasing the availability of replacement stock and production of milk. It is therefore, suggested that implementation of improved calf and farm management practices and proper environmental protection in the study areas would significantly reduce calf mortality and morbidity.
Efficacy of Purified Glutathione -S- Transferee in Providing Protection against Haemonchus Contortus Infection in Sheep
The efficacy of purified glutathione-s-transferase (GST) to protect sheep against haemonchosis was assessed. Fifteen lambs, 3.5-5month-old, were bisected into five groups (n=3). Two groups were vaccinated with 250μg of each prepared antigen (crude adult antigen (CAA), purified GST) in combination with mineral oil adjuvant at zero, 14th days of the experiment. The other three groups were kept as control groups; non-immunized infected, non-immunized non-infected and adjuvant control. One week after the last booster dose, the lambs in all groups except control negative were challenged with 400 L3/kg live weight orally. Protein characterization of each antigen was done by SDS-PAGE and immunoblting. The vaccine efficacy was estimated by the fecal egg count, where the vaccinated groups showed 58.90% and 79.38% reduction in eggs in CAA and GST respectively. Moreover, 31.45% and 92.10% reduction in abomasal worm count was reported in CAA and GST vaccinated lambs, respectively. The protection was found corresponsive to the sera antibody levels in the immunized groups. It was concluded that the GST had protective efficacy against H. contortus infection in lambs.
Viruses are obligate intracellular parasites that require living cells in order to replicate. Cell culture for propagation and identification of viruses is an important component of the clinical virology laboratory. In general, diagnostic tests can be grouped into three categories: direct detection, virus isolation and serology. Direct examination methods can usually give a result either within the same or the next day. Immunofluorescence is widely used for the rapid diagnosis of virus infections by detection of virus antigen in clinical specimens and detection of virus-specific antibodies.
Aim: The aim of the study was to evaluate the levels of serum 25(OH)D levels in patients to identify the average vitamin D levels, the incidence of insufficiency and deficiency amongst the patients reporting to a dental hospital in Mumbai. Methods: Serum 25(OH)D levels of 100 randomly selected patients who reported to Nair Hospital Dental College in Mumbai were assessed to evaluate the vitamin D status. Serum levels below 10 ng/mL were categorized as “deficiency “and between 29-11ng/mL were categorized as “insufficiency” of vitamin D.
Validation of an Anti-Protective Antigen ELISA for Quantitative IgG Evaluation in B. anthracis Immunized Horses
The potency test for anthrax vaccines has historically involved the challenge of actively or passively immunized laboratory animals with a fully virulent strain of Bacillus anthracis. Lethal challenge studies with the archetypal virulent strains such as B. anthracis Ames strain present considerable difficulties in laboratory management and handling and are too inefficient for the early evaluation of alternative preventative and therapeutic interventions. An ELISA for the evaluation of antibody response to protective antigen (PA) in horses immunized with the Sterne 34F2 strain spore vaccine was developed. The objective of this work was to study the performance of this assay in terms of the guidelines set forth by the International Conference on Harmonics (ICH) and the Center for Biologics Evaluation and Research (CBER) for analytical procedures. We have demonstrated a working range for this assay (73-1581 EU/ml) on the bases of the following parameters: linearity (25 and 1,662 EU/ml, r2 = 0.9988, p < 0.001), accuracy (94.8 - 105.4 %, recovery within the range of 25 and 1,662 EU/ml), precision (≤ 17.6 % CV, repeatability; ≤ 15.7 and ≤ 13.1 % CV, intermediate precision per day and per analyst, respectively), limit of detection (2.25 EU/ml) and limit of quantitation (25 EU/ml). The assay was also demonstrated to be specific for the evaluation of anti-PA IgG antibodies. Based on the assay performance characteristics it was determined that the assay was adequate for use in B. anthracis immunogenicity testing in horses.
Field Evaluation and Use of a Non Commercial Peptide Enzyme Immunoassay for Human Immunodeficiency Virus Serotyping in Abidjan (Ivory Coast)
In West Africa, where Human Immunodeficiency Viruses 1 and 2 (HIV-1 and HIV-2) co-circulate, serological assays allowing the reliable serotyping of HIV infection are needed.
Camelpox is routinely diagnosed based on clinical signs, pathological findings and cellular and molecular assays.
Fifteen Sahel goats were randomly allocated into three groups A, B and C to evaluate Serum Haptoglobin (Hp) profiles following rumenotomy as markers of surgical stress using Quantitative ELISA.
Acquired Immune Deficiency Syndrome (AIDS) is a spectrum of conditions caused by infection with the human immunodeficiency virus (HIV). They get it after being infected with the HIV virus. HIV (human immunodeficiency virus) is a retrovirus that primarily infects components of the immune system.
Hydatid disease is caused by the larval form of parasitic tapeworm; Echinococcus granulosus. Primary spinal hydatid disease is rare. Primary bone localization is rare and it accounts between 0.5% and 4%. Spinal localization accounts for less than 1%. The infection may be misdiagnosed initially.