Articles Related to Embryo

Maize is an economically important crop worldwide. As the world population increases, there is a great need to develop high yielding and stress resistant maize varieties. To provide a future platform for maize improvement, we tested mature embryos as alternative explants for the regeneration of three economically important maize inbred lines: CML331, CML442 and CML444.

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Penicillin and streptomycin are the most commonly used to prevent bacterial infection or contamination in cell culture. However, the role of Penicillin-streptomycin on inducing mouse embryonic stem cells(mESCs) to differentiate into cardiomyocytes is unclear. To illustrate the mechanism of penicillin-streptomycin on inducing mESCs differentiation into cardiomyocytes, we firstly investigated mESCs differentiation into cardiomyocytes by the traditional method. The time- space expressions of cardiac-specific genes were detected by real-time PCR and western blotting at 8 different time-points over a period of 36 days. Furthermore, action potentials were measured by patch clamp for mESC-derived cardiomyocytes. Embryonic bodies through hanging drop method, were incubated with penicillin-streptomycin (P/S) at different concentrations (1×PBS, 1×, 2×, 5×), and then, the number of cardiomyocytes and expression levels of myocardial marker proteins were detected by flow cytometry, cellular immunofluorescence, and western blotting, respectively. We found that the SSEA-1 of mESC presented green fluorescence, and H.E. staining showed karyoplasmic ratio >>1. Besides, TNNI3, TNNT2, ACTN2 and MYL-2v were clearly visible by cellular immunofluorescence. Furthermore, the ACTN2 positive cells rate of 2× and 5× groups were remarkably higher than that in Cntl (1×PBS) group and 1× group (10 Units/mL penicillin; 10 μg/mL streptomycin), as well as the expressions of GATA4, MYL-2v, TNNI3, ACTN2

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There has been little advance in embryo selection criteria since the first IVF. To this day, we select embryos by morphological criteria, which provide little information as to the actual embryo quality.

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Rainfall patterns are recognised as important for normal ecosystem functioning in arid environments. Most attempts made to understand the effect of rainfall on amphibian ecology have focused on long-term rather than short-term trends. Here, factors effecting embryonic mortality and clutch size of the African foam nesting frog Chiromantis xerampelina were examined at two ponds from April – June in 2011-2012 in Malawi. A total of 73 foam nests were monitored following spawning. On average 29% of eggs in 2011 and 26% in 2012 suffered mortality from both ponds and this mortality significantly varied between nests. Linear regression showed that the date of spawning had a significant effect on mortality due to moisture requirements of the eggs. The height of nests over the water surface had a negative impact on clutch size and increased rainfall decreased egg mortality in both years at both ponds. In a changing climate, with rainfall projected to become more infrequent in this region, organisms may not be able to rely on rainfall patterns as cues for reproduction, which may have a negative impact on amphibian populations.

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Camelpox is routinely diagnosed based on clinical signs, pathological findings and cellular and molecular assays.

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In assisted reproduction of cattle the design and preparation of in vitro culture media has been instrumental in supporting the development of bovine oocytes and embryos. In vitro production (IVP) involves three main sequential steps: oocyte aspiration and in vitro maturation (IVM); in vitro fertilization (IVF); and in vitro culture (IVC) of early pre-implantation embryos.

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Cleft lip is a common craniofacial anomaly. Median cleft lip is a midline vertical cleft through the upper lip. Of all the types of clefts, minimal Median cleft of the upper lip is a very rare craniofacial anomaly.

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In relation to assisted mammalian reproduction, the goal of cryopreservation is to preserve without significant loss of viability a stock of gametes and/or embryos with a view to thawing those cells for use in in vitro reproduction treatments. There are numerous cryopreservation protocols, which vary in terms of cryoprotectant used, storage temperature, freezing and thawing rates, and the particular cells that they are suitable for preserving.

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Assisted reproductive technologies, such as artificial insemination and embryo transfer, have produced significant outcomes in cattle. These technologies are useful not only for improving fertility but also for increasing the rate of genetic change in beef and dairy livestock. Today, embryo transfer in cattle is both practical and commercially viable. However, bovine embryo transplantation still remains suboptimal under on-farm conditions, one of the main reasons for which may be inefficient nutritional management.

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Motor Neuron Diseases (MND) are a group of progressive neurological disorders that destroy motor neurons, cells that control essential muscle activity. Despite the advances in treatment modalities, the overall survival rate has not changed for decades. This is mainly due to the lack of effective methods.

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The Congenital Rubella Syndrome is a multisystemic disease and CNS involvement occurs in the form of microcephaly, mental/motor disabilities, leptomeningitis, encephalitis, vascular damage and retardation of myelination. We report a case of gross non-communicating hydrocephalus in a neonate of Congenital Rubella Syndrome which is a rare presentation.

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Cryopreservation is the use of ultra-low temperatures to preserve whole living cells and tissues in order to retain their structural integrity and maintain their physiological viability. It enables long term storage of cells in order to circumvent the need for continuous in vitro culture. When cryopreserving bovine embryos there are two means of cryopreservation: slow programmable freezing and vitrification. While controlled-rate and slow freezing can be applied widely to in vivo derived-embryos, this methodology remains less successful for embryos produced in vitro.

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Basal cell carcinoma is the most frequent malignant tumor of the skin, and the most common carcinoma found in some countries. The main clinical subtypes of BCC are nodular, superficial, pigmented and morpheaform. The majority of the lesions appear on the head and neck, with a particular predilection on the upper central part of the face.

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The international nomenclature of the anatomical, histological and embryological terms is known as Nomina Anatomica Veterinaria (N.A.V.), Nomina Histologica Veterinaria (N.H.V.) and Nomina Embryologica Veterinaria (N.E.V.).

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