Articles Related to LC

Acetylsalicylic acid (ASA), a well-recognized non-steroidal anti-inflammatory drug (NSAID), is well known for the treatment of fever, pain alleviation and inflammatory conditions, especially for the prevention of cardiovascular complications and disorders. However, ASA has been reported to cause hepato- and gastro-toxicities.

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We present a case of total esophageal reconstruction with a free Jejunal flap in a patient with a history of esophagectomy and two previous failed attempts at reconstruction. Despite the technique here described offers a reliable option when the stomach and colon are not available, the success of the reconstruction might be compromised by the onset of a perforated ulcer at the gastric blind pouch related to previous failed gastric pull-up (GPU) After a major reconstructive surgery of long hours, gastric pouch may develop ulcer with perforation and fatal empyema due to stress, although the severe complication is rare.

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Background: Recently the Dutch Health Council has issued a revised alcohol guideline stating: Do not drink alcohol, or if any only drink one glass daily. The current guidelines are intended to obtain the lowest disease burden of the most burdensome diseases in The Netherlands. The current paper aims to estimate the overall impact of alcohol consumption on these diseases.

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Colpodella species are free-living alveolates that possess an apical complex used for attaching to eukaryotic prey protists for ingestion of the cytoplasmic contents of the prey. Colpodella sp. are the closest relatives of the Apicomplexa, a phylum that includes the important human pathogens Plasmodium falciparum, Toxoplasma gondii and Cryptosporidium parvum. In this study, we investigated morphological characteristics of Colpodella (ATCC 50594) in a diprotist culture containing Bodo caudatus as prey in order to identify features differentiating both protists. The level of apical complex protein conservation among free living alveolate relatives of apicomplexans and intracellular apicomplexan pathogens is unknown. Antibodies against proteins of the apical complex in Colpodella sp. are currently unavailable. We performed staining and immunological characterization of Colpodella in a diprotist culture containing B. caudatus to aid routine differentiation of predator and prey in culture. Staining revealed distinguishing morphological features of both protists. The kinetoplast in B.caudatus was identified using Giemsa staining and was used to differentiate B. caudatus from Colpodella sp. trophozoites.

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Synthetic cannabinoids (SCs) present a multitude of problems in terms of maintaining up-to-date methods of detection. They are novel psychoactive substances originally synthesized for medical use and research purposes. Abuse has demonstrated a variety of effects ranging from euphoria to aggressive behavior and death. The marketing, similar naming, and described pharmacological interactions create the dangerous and false perception that SCs are similar to, or the same as, tetrahydrocannabinol in cannabis products

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Accurate measurement of ergocalciferol in biological samples has become easier using high-end mass spectra. Currently, various LCMS/MS methods have been developed to quantify ergocalciferol. Use of LC-MS/MS has accomplished to develop an accurate method that could quantify ergocalciferol in a large number of samples where it should be analyzed in the blood immediately after dosage. The objective of this study is to optimize chromatographic conditions and evaluate the LC-MS/MS method for accurate quantification of the dosage form of ergocalciferol in human plasma. Extraction and separation of ergocalciferol in human plasma was achieved using methanol liquid-liquid extraction and by a reverse phase C8 column, respectively

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Tertiary education has always been regarded as highly stressful environment to students. Medical and health science training further add to the already stressful environment. Awareness of the existence of stress in medical students by physicians will help in diminishing student’s experience of stress. Therefore, identifying additional stressors in the clinical context is very crucial for providing measures to minimize students’ stress to a tolerable level and helping them to cope better. And the aim of this study is to assess perceived stress and factors associated with it among Bahir Dar University medical students, North West Ethiopia, 2016.

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Spasm provocation tests (SPTs) using a pressure wire and compared the results with those from patients who underwent the same test without a pressure wire.

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Marchiafava-Bignami disease (MBD) is a neurological disorder that has been found to be associated with chronic alcoholism and malnutrition. We report a 45 year old man, chronic alcoholic that developed discouragement for activities involving daily living, changes in retrograde memory in addition to mutism and gait instability. Brain MRI showed central atrophy of the corpus callosum(CC), hypointensity(necrosis) and ventricular dilation(white matter and subcortical region involvement).

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France, Greece, Italy, and Spain the major sheep and goat milk producers in Europe, got the initiative through an interim of 30 years (1985 – 2015), to organize - through IDF - 7 symposiums aiming at the upgrade of the Dairy Sector. One major interest was the milk quality. Stepwise, the efforts followed cow milk structural development in organizing the testing laboratory, the definition of quality in composition (fat, protein, lactose, total solids), in Hygienic (Total Bacteria Count, Somatic Cell Count, antibiotics residues and Aflatoxin M1 contamination), adulteration with water and detection of milk species. EU, by Decision 2002/657/EC, defined the rules for the Directive 96/23/EC, enumerated the testing methods, the demands in sampling procedures, the minimum limits of performance, the quality control (ISO 17025) and the interpretation of results. The equivalency of results from different laboratories, through ISO 17025, demanded the application of ISO analytical standards.

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The objective of this study is to assess the relationship between food intake, nutritional status and good school performance among schoolchildren in the district of Abidjan. A cross-sectional study was carried out from December 2015 to April 2016 in 426 schoolchildren in Yopougon and Bingerville, two communes in the district of Abidjan. The nutritional status was defined starting from the BMI and calculated with Who Anthro Plus v1.0.4. The student and chi-square tests were used to compare the numbers and proportions. Binary and multiple logistic regressions have been used to see the association between good academic performance and the quality of food consumed and socio-demographic characteristics.

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Streptomyces species are medically, industrially, and microbiologically important bacteria that are found in soils and other habitats. Streptomyces isolates and QUBC97 were cultured on a new medium (CCG). Agar plug diffusion test showed antibacterial activity. N-butanol extracts analysed by silica thin layer chromatography (TLC), HPLC purified and identified by MS analysis. QUBC97 was identified based on morphology, PCR, and DNA sequencing of two loci.

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Patients with Inflammatory Bowel Disease (IBD) not responding to steroid treatment should be considered for immunomodulator therapy. The risk of lymphoma in IBD patients treated with immunomodulators is a well-documented but rare complication and remains a subject of controversy. Here, we present two patients treated successfully in outpatient clinics on thiopurine immunomodulators who were admitted within weeks of each other with a diagnosis of lymphoma.

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Helicobacter pylori (HP) infection is present in more than the half of the world population. Most individuals are infected during early childhood; in developing countries, 50% of children are infected by the age of 5 years [1,2]. Studies suggest the main etiological role of HP infection in development of chronic gastroduodenal pathology and the formation of its most severe forms in patients of different age groups. However, not all infected people exhibit diseases associated with this bacterium. In addition, pathogenic mechanisms of duodenal ulcer disease (DUD) in childhood remain not fully understood.

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Hemoglobin A2 (Hb A2) is a minor component of the hemoglobin present in normal adult red blood cells, accounting for 1.5-3.5% of the total hemoglobin in healthy individuals.

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Vertical Cu2ZnSnS4 (CZTS) nanowire arrays have been synthesized via two-step electroplating method into anodized aluminum oxide template. For deposition of CZTS nanowires, anodized aluminum oxide (AAO) was used as the growth mask for the growth of the nanowires. AAO templates with hole sizes of 70 nm in diameter were used in the experiments.

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