Articles Related to PCR
Association of ACE I/D rs4646994 and ACE2 rs2285666 Receptor Gene Polymorphisms with SARS-CoV-2 Infection in Bangladeshi People
Covid-19 caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which hit the world
in December 2019 is one of the worst medical emergencies people have ever encountered. Human angiotensin I converting
enzyme 2 (ACE2) is the entry path of this virus to the host that regulates the renin-angiotensin-aldosterone system (RAAS).
Hypothesis: Susceptibility to SARS-CoV-2 infection may be affected by ACE rs4646994 and ACE2 rs2285666 polymorphisms.
Optimization of a Conventional PCR Assay for the Identification of Corynebacterium Pseudotuberculosis from Pyogenic Lesions
Caseous lymphadenitis (CL) is a pyogenic disease that is caused by zoonotic bacteria and is in the same family as Corynebacterium diphtheriae (the etiologic agent of human Diphtheria).
“Only Cigarette Butt is Left, DNA Fingerprinting Traps the Theft”
The examination of saliva traces left on cigarette butts as evidences are complicated due to the availability of biological material in trace
amounts and its rapid degradation due to extreme effects of environmental factors. This study is aimed to assess the DNA purity and
quantify the amount of DNA preserved in saliva found on cigarette butts subjected to various temperatures and humidity. Isolation of cell
material from biological traces on forensic evidence is often a serious challenge to solving forensic cases.
Detection of the host-specific Bacterioides and Bifidobacteriumsaeculare markers in surface water in Hanam, Vietnam with real-time PCR
The microbiological quality of coastal or river waters can be affected by faecal pollution from human or animal sources. Contamination of fresh produce with pathogenic bacteria can be attributed directly or indirectly to the raw wastewater (domestic, industrial effluents, human and animal excrement). This includes water sources that are routinely used for crop irrigation and produce washing. An efficient MST (Microbial Source Tracking) toolbox consisting of several host-specific markers would, therefore, be valuable for identifying the origin of the faecal pollution in the environment and thus for effective resource management and remediation. As a result, the need for expanding from traditional techniques to molecular methods has been highly recommended.
Scardovia Wiggsiae and the Other Microorganisms in Severe Early Childhood Caries
Severe early childhood caries (S-ECC) is an aggressive form of tooth caries in preschool children. New different bacteria were defined as associated with S-ECC. The aim of this study was to analysis the role of Scardovia wiggsiae and the other cariogenic microorganisms in S-ECC in Turkish children. Two same-sized groups of children were enrolled in the study: the first group consisted of 40 children with S-ECC (mean-age:52.93±11.5months), and the second group consisted of 40 caries-free children (mean-age:49.43±12.47months). The numbers of mutans streptococci, lactobacilli and yeasts were examined by culturing techniques, while the presences of S. wiggsiae, Streptococcus mutans, Streptococcus sobrinus, Actinomyces gerencseriae, Veillonella parvula, Fusobacterium nucleatum were examined by using 16S rRNA-based PCR technique in plaque samples. The prevalence of S. wiggsiae, S. mutans, A. gerencseriae, V. parvula and the numbers of mutans streptococci, lactobacilli, yeasts were found significantly high (P<0.05) in SECC. Also, the presence of S. wiggsiae with either S. mutans, A. gerencseriae, V. parvula or F. nucleatum were associated (P<0.01) with S-ECC. Understanding the effects of bacteria on initiation and progression of S-ECC will also help to improve protective and preventive treatments.
Blonanserin N-Oxide Lowers Glucose Levels in Animal Models
The N-oxide of anti-psychotic drug Blonanserin (BLNO) was synthesized and tested as a novel molecule to understand its ability in
reducing blood glucose levels.
Association between Insertion/Deletion Polymorphism of ApoB Gene with Dyslipidemia and Obesity Risk in Patients with Type 2 Diabetes
Several studies have suggested that Insertion/Deletion polymorphism of ApoB gene is associated with obesity, dyslipidemia, diabetes and coronary heart disease (CHD).
Congenital Dyserythropoietic Anemia Type III and Primary Hemochromatosis; Coexistence of Mutations in KIF23 and HFE
Congenital dyserythropoietic anemia type III (CDA III) can be caused by mutation in KIF23. CDA III differs from CDA I and II in the sense that secondary hemochromatosis has not been reported. However, we have observed elevated serum ferritin in a CDA III family.
Adipogenesis in Obesity is Modulated by IP6 in Peanuts through Activation of the Nuclear Receptors (PPARs)
Increased life style changes have led to global epidemic of obesity. The global prevalence of obesity protracts to increase, with devastating consequences for overall health. Thus by exploring novel beneficial properties of natural products and their scientific indications are the need of the hour and can bring about a whole new revolution in nutraceutical industries and health care sectors.
Assessment of Rumen Microbial Adaptation to Garlic Oil, Carvacrol and Thymol Using the Consecutive Batch Culture System
Although plant derivatives have shown promise in reducing enteric methane (CH4) emissions from ruminants in short-term studies, investigation on possible rumen microbial adaptation to these compounds is still limited. The objective of this study was to assess the possibility of mixed rumen microbial adaptation to antimethanogenic plant derivatives over relatively long-term in vitro incubation. Treatments were: garlic oil, carvacrol and thymol, each at a final concentration of 300 mg/l.
Extraction of Mastitis Pathogen DNA from Sample Collecting Cards: Practical Consequences
Shipment of liquid milk samples for bacteriological examination is a limitation to large scale epidemiological studies. Sampling bulk tank milk (BTM) with sample collection cards (WhatmanTM FTATMMiniCard) was identified as an interesting procedure to ease sample collection and shipment.
Persisting Cough as the Single Presenting Symptom of an Intrathoracic Tumor in a Nine-Month-Old Child with Adenovirus Airway Infection
We report on a nine-month-old girl who presented with persisting cough, and diminished ventilation of the left hemithorax. Viral pneumonia was suspected after Adenovirus detection by PCR, but chest X-rays showed a persistent shadowing of the left hemithorax and persistent coughing despite clinical improvement. Because of the discrepancy between clinical and radiological signs further investigations by ultrasound and CT scan were performed, which visualized an intrathroracic tumor. Histopathology confirmed
diagnosis of a teratoma.
Meningoencephalitis due to Enteroviral Infection – An Often Overlooked Etiology
Enteroviruses are responsible for causing several recent well-publicized outbreaks in the United States, including gastrointestinal and upper respiratory infections. Enteroviruses are also capable of infecting the central nervous system, leading to manifestations of meningitis and encephalitis. Because enteroviruses are often overlooked as the etiology of CNS infections, we explore data from national surveillance, typical presentation and diagnostic information, as well as patterns of infection and transmission in an effort to remind practitioners to be mindful of this frequent cause of infection and its propensity to be spread from person to person.
Clinical and Molecular Evaluation of a Case of Male Infertility and Azoospermia
Here we describe the case of a 35 year old male, with a harmonic phenotype, who sought medical assistance to assess the etiology of his infertility. As a result of clinical examination, karyotyping, FISH and molecular studies, we obtained the following karyotype: mos 45,X[18]/46,XY,idic(Y)q(11.2)[82]. Furthermore, the Y chromosome was characterized by the absence of the regions AZF2 (former AZFb, within the 11.22 band, and AZFc, within the 11.23 band), whereas the region AZF1 was conserved. Male infertility can be caused by several genetic alterations.
Editorial Board Members Related to PCR
Gillian Tully
Director
Principal Forensic Services Ltd
United Kingdom
Principal Forensic Services Ltd
United Kingdom
SANJEEV KUMAR SINGH
Professor
Department of Bioinformatics
Alagappa University
India
Department of Bioinformatics
Alagappa University
India
Jagriti Narang Malhotra
Assistant Professor
Amity University
Noida
India
Amity University
Noida
India
Md Mahmudul Hasan Khan, PhD
Genetics and Breeding Unit, Laboratory of Climate-Smart Food Crop Production, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, UPM, Malaysia.
&
Scientific Officer (Genetics & Plant Breeding)
Bangladesh Agricultural Research Institute (BARI)
Under Ministry of Agriculture (MOA), Bangladesh.
Jayasimha Rao
Associate Professor
Jefferson College of Health Sciences
Department of Medicine
USA
Jefferson College of Health Sciences
Department of Medicine
USA