Articles Related to TOF

Pyoderma gangrenosum is a rare ulcerating skin disease often presenting as an extra intestinal manifestation of IBD and is difficult to manage. Treatment options for pyoderma gangrenosum include steroids, calcineurin inhibitors and anti TNF agents. Here in, we report a case of recurrent Pyoderma gangrenosum with Ulcerative Colitis that was successfully managed with Tofacintib, a JAK inhibitor

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Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has become a widely used technique for the rapid, accurate, durable, economical, and trustworthy method for identification of bacteria, mycobacteria and fungal pathogens in the microbiology laboratory.

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The ability to measure structural and functional alterations in cellular and tissue lipids with small footprint, accessible instrumentation has sparked interest in their role in disease pathology. However, various lipidomic analytical tools tend to be cumbersome and time-consuming. A rapid, accurate, and straight forward peak alignment software routine would greatly facilitate the analysis of large datasets, such as those produced by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Herein, we describe a novel Rapid Peak Alignment Method (RPAM) which allows untargeted analysis of lipids expressed in brain white matter following chronic ethanol exposure in an established experimental model. The RPAM outputs data comparable to manual peak alignments but the processing time requires only 90 minutes instead of 8-10 hours. This method is readily adapted to a broad range of models, tissue types, and human diseases.

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Dermatofibroma (DF) is a common benign fibrosing lesion that can be found on any surface of the body, most often in the lower extremities. Dermatofibroma that arises on the digits, as in our present case, may be confused with other clinical entities or even malignant tumors, leading to unnecessarily aggressive treatment. To ensure proper diagnosis and treatment, dermatofibroma should be considered in the differential diagnosis of digital papules.

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This was a 50-year-old patient with recurrent bilateral nodular anterior scleritis refractory to several treatments, including immunosuppressants and anti Tnfα. She was assessed for the different causes of immune-mediated and infectious diseases, but nothing was found. Tofacitinib was started 5 mg twice a day, which led to the spacing of crises until their total disappearance. At present, the patient has been crisis-free for nine months. In conclusion, the use of Janus Kinase inhibitor (Tofacitinib) alone was effective in the treatment of refractory recurrent nodular anterior scleritis.

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Dermatofibrosacroma protuberans of abdominal wall is very rare. Surgery is treatment

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T cell acute lymphocytic leukemia (T-ALL) is an aggressive hematologic malignancy in terms of its pathology and populations. It is important to understand the phenomenon of autofluorescence in living cells because normal and cancer cells can be distinguished by this feature. However, the autofluorescence link to T-ALL is poorly understood.

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1-D, 2-D gel electrophoresis, mass spectrometry and bioinformatics analysis were established and utilized to examine the changes in protein expressions associated with germination of Ceiba pentandra seeds.The change in protein pattern was analysed by 1-D and 2-D gel electrophoresis for better separation and to ascertain the pattern of protein mobilization during germination.

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Use of gas chromatography (GC) combined with time-of –flight mass spectrometry (TOFMS) with soft ionization generated with three different plasma gases (i.e., Xe, Kr, Ar) was evaluated for the identification of several pyrrolidinophenone-type designer drugs that are commercially available.

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Gas chromatography-mass spectrometry (GC-MS) with electron ionization (EI) or chemical ionization (in positive or negative mode) plays an important role in the forensic analysis of designer drugs.

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In recent years, an increasing number of new designer-drugs have increased the demands for general toxicological screening [1]. Limited screening based on immunoassays is commonly used in clinical toxicology, whereas more comprehensive approaches are common in forensic toxicology such as screening based on Gas or Liquid Chromatography (GC or LC) approaches.

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An Ultra-High Pressure Liquid Chromatography Time-of-Flight Mass Spectrometry (UHPLC-TOF-MS) method for quantitative analysis of 30 drugs in whole blood was developed and validated. The method was used for screening and quantification of common drugs and drugs of abuse in whole blood received from autopsy cases and living persons.

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