Articles Related to Validation

This paper covers the validation and measurement uncertainty evaluation of an ICP-AES method for bismuth mass fraction determination in bismuth sulfide thin films. Plasma view at radial mode and the wavelength were monitored for bismuth at 223.061 nm. Following AFNOR (Association Française de Normalisation) Norm NFT 90-210 (2009), a regression equation was calculated using single element bismuth standard solution prepared in the same matrix as samples. The linear range was between 0.2 and 2.0 mg L−1. Limits of detection (LOD) and quantification (LOQ) were calculated as 0.03 and 0.12 mg L−1, respectively. Recovery percentages were ranging between 91 and 109 %. Accuracy was found to be adequate. Estimated expanded uncertainty according to both GUM and EURACHEM guides was 3.1 %.

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Non-uniform native T1 (T10) distribution influences reliable measurement of Ktrans: a pharmacokinetic parameter (Ktrans) that quantitatively measures neovascularization of the tumor tissue that has been used for the classification of malignant breast lesions and to monitor therapy response.

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The present research deals with the development of a stability indicating reverse phase HPLC with PDA detector method for the determination of Armodafinil Agilent XDB-C18, 150×4.6mm, 5µm or Equivalent column. The present research deals with the development of a stability indicating reverse phase HPLC with PDA detector method for the determination of Armodafinil Agilent XDB-C18,

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Neospora caninum is a world-wide distributed apicomplexan parasite, causative agent of bovine neosporosis, which is one of the major causes of reproductive losses in cattle affecting both dairy and beef industries. Several techniques are regularly used for the diagnosis of bovine neosporosis.

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BioequivalenceA sensitive ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS) method for measurements of N-butylscopolamine in plasma was developed and validated. A SPE extraction was proposed for the clean up of plasma and N-butylscopolamine-d9 was added as internal standard. The analyses were carried out using a phenyl column and mobile phase of acetonitrile:

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Despite hypertension is a major cardiovascular risk factor; only a few patients are adherent to therapy. Therefore, it is essential the acknowledgment of factors affecting adherence. The development and validation of a scale assessing factors affecting patients’ adherence to the therapeutic regimen.

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The potency test for anthrax vaccines has historically involved the challenge of actively or passively immunized laboratory animals with a fully virulent strain of Bacillus anthracis. Lethal challenge studies with the archetypal virulent strains such as B. anthracis Ames strain present considerable difficulties in laboratory management and handling and are too inefficient for the early evaluation of alternative preventative and therapeutic interventions. An ELISA for the evaluation of antibody response to protective antigen (PA) in horses immunized with the Sterne 34F2 strain spore vaccine was developed. The objective of this work was to study the performance of this assay in terms of the guidelines set forth by the International Conference on Harmonics (ICH) and the Center for Biologics Evaluation and Research (CBER) for analytical procedures. We have demonstrated a working range for this assay (73-1581 EU/ml) on the bases of the following parameters: linearity (25 and 1,662 EU/ml, r2 = 0.9988, p < 0.001), accuracy (94.8 - 105.4 %, recovery within the range of 25 and 1,662 EU/ml), precision (≤ 17.6 % CV, repeatability; ≤ 15.7 and ≤ 13.1 % CV, intermediate precision per day and per analyst, respectively), limit of detection (2.25 EU/ml) and limit of quantitation (25 EU/ml). The assay was also demonstrated to be specific for the evaluation of anti-PA IgG antibodies. Based on the assay performance characteristics it was determined that the assay was adequate for use in B. anthracis immunogenicity testing in horses.

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Iron is an important component of hemoglobin, in addition to other positive roles like proper growth and development of humans and for growing of fetus in pregnancy women. Iron deficiency anemia is more prevalent among vegetarian than non vegetarians.

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To develop and determine validity and reliability of the Knowledge, Attitude and Practice on Healthy Lifestyle Questionnaire (KAP-HLQ) among lower secondary adolescents.

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Skin fold thickness prediction equations are widely used for estimating body fat percentage and thereby obesity. Equations to estimate body fat percentage for the Asian Indian population do not yet exist. The main purpose of this study was to develop and validate a new prediction equation for the estimation of body fat percentage in Asian Indian adults using anthropometric measurements (skin folds, circumference, bone breadth) and DEXA.

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The palatal rugae can be an alternative method of forensic identification. Through the years, several investigations focused on the effect of orthodontic treatment in the palatal rugae pattern.

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The Novel, simple, sensitive, rapid, accurate and economical and reliable First derivative spectroscopic method has been developed for synthetic mixture of Dapagliflozin (DAPA) and Metformin hydrochloride (MET).

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A new, stable and accurate high performance liquid chromatography ultraviolet spectrophotometric method for quantitation of Ciprofloxacin in human plasma was developed and validated.

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An Ultra-High Pressure Liquid Chromatography Time-of-Flight Mass Spectrometry (UHPLC-TOF-MS) method for quantitative analysis of 30 drugs in whole blood was developed and validated. The method was used for screening and quantification of common drugs and drugs of abuse in whole blood received from autopsy cases and living persons.

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