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Articles Related to antigen

Validation of an Anti-Protective Antigen ELISA for Quantitative IgG Evaluation in B. anthracis Immunized Horses

The potency test for anthrax vaccines has historically involved the challenge of actively or passively immunized laboratory animals with a fully virulent strain of Bacillus anthracis. Lethal challenge studies with the archetypal virulent strains such as B. anthracis Ames strain present considerable difficulties in laboratory management and handling and are too inefficient for the early evaluation of alternative preventative and therapeutic interventions. An ELISA for the evaluation of antibody response to protective antigen (PA) in horses immunized with the Sterne 34F2 strain spore vaccine was developed. The objective of this work was to study the performance of this assay in terms of the guidelines set forth by the International Conference on Harmonics (ICH) and the Center for Biologics Evaluation and Research (CBER) for analytical procedures. We have demonstrated a working range for this assay (73-1581 EU/ml) on the bases of the following parameters: linearity (25 and 1,662 EU/ml, r2 = 0.9988, p < 0.001), accuracy (94.8 - 105.4 %, recovery within the range of 25 and 1,662 EU/ml), precision (≤ 17.6 % CV, repeatability; ≤ 15.7 and ≤ 13.1 % CV, intermediate precision per day and per analyst, respectively), limit of detection (2.25 EU/ml) and limit of quantitation (25 EU/ml). The assay was also demonstrated to be specific for the evaluation of anti-PA IgG antibodies. Based on the assay performance characteristics it was determined that the assay was adequate for use in B. anthracis immunogenicity testing in horses.
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Detection of Anti-Trypanosoma Cruzi Antibodies among Donors at A Blood Bank from Southern Mexico, Using an Iron Superoxide Dismutase Excreted (Fe-Sode) as Antigen

Trypanosoma cruzi, the causal agent of Chagas disease in humans, is a widely spread protozoan in Latin America. Chronically infected people are asymptomatic during an indeterminate stage but can represent a significant risk of transmission due to blood donations and organ transplants. Blood transfusion is recognized as the second most important path for transmitting of Chagas disease
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The Treatment of Symptoms in Atopic Dermatitis as a Superantigen Disease

This research proposed a treatment for the symptoms of the disease – skin rash, intestinal tract and cervical spine disorders. The treatment proposed (by the use of combined disinfectants) proofed effective for the treatment of skin rash. The researchers hope that this treatment will be adopted by health practitioners. Further study could look into the treatment of skin rash, along with intestinal tract and cervical spine disorders.
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Costimulatory Molecules CD80 and CD86 Colocalized in Neutrophil Extracellular Traps (NETs)

Neutrophil extracellular traps (NETs) have been proposed as vehicles for the sensitization of T cells by decreasing their activation threshold.
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Increased High Mobility Group Protein A2/SMAD3 Relates to Ovarian Cancer Progression

The high mortality associated with ovarian cancer is generally related to the development of drug-resistant disease. HMGA2 protein, a member of the high-mobility group AT-hook (HMGA) family of non-histone chromatin binding factors, is overexpressed in high-grade serous ovarian and tubal carcinomas, though little is known about its contribution to disease progression and drug resistance. We sought to assess whether compositional changes in HMGA2 production were associated with ovarian cancer progression.
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Helicobacter pylori Infection and its Potential Association with Idiopathic Hypercalciuric Urolithiasis in Pediatric Patients

A total of 150 patients categorized into 100 cases (urolithiasis-positive) with urinary stone disease, aged from 5 to 18 years, and met the characteristics of idiopathic urolithiasis in children as well as 50 controls (urolithiasis-negative) that had relatively similar demographic criteria except for idiopathic urolithiasis.
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Development of Severe Hypophosphatemia from Acquired Fanconi Syndrome during Treatment with Abiraterone

The first patient was a 77 year old man who had been on abiraterone for 12 months before his phosphorous was checked. At that point, it was 0.6 mg/dl, which is severely decreased. A full work up for hypophosphatemia showed normal parathyroid hormone and 25-hydroxyvitamin D. A urine study showed inappropriate excretion of phosphorous along with aminoaciduria and normoglycemic glucosuria.
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Acute Type C Botulism with Fatal Consequences in a Holstein Breeding Establishment in Northern Italy

BOTULISM is a neuro-paralytic intoxication illness caused by the ingestion of neurotoxins of Clostridium botulinum with contaminated water or food. The Gram-positive spore-producing bacterium Cl. botulinum is found worldwide and can survive in spore form for up to 30 years in numerous substrates in the environment. Cl. botulinum is classified into 7 or 8 different types (A, B, C (C1, C2), D, E, F, G) depending on the antigen properties of the botulinum neurotoxins (BoNT) produced, with intoxication mostly appearing in cattle following the ingestion of neurotoxins of type C and D and, less frequently, of type B.
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Study on Efficiency of Protein Extractants Employed for Human Origin Determination of Blood

Human origin determination is an important aspect of blood grouping analysis in forensic science laboratories. In the present study, protein extractants like gel buffer, ammonia and saline employed for origin determination were evaluated and compared qualitatively and quantitatively for their role in the extraction of proteins from dried blood stained materials of human origin at regular time intervals.
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An Interesting Coexistence of a Classical-Nodular and an Adenoid-Ulcerous Basal Cell Carcinoma in the Same Anatomic Location

Basal cell carcinoma is the most frequent malignant tumor of the skin, and the most common carcinoma found in some countries. The main clinical subtypes of BCC are nodular, superficial, pigmented and morpheaform. The majority of the lesions appear on the head and neck, with a particular predilection on the upper central part of the face.
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Molecular Mechanisms of Mismatch Repair Genes in Cancer – A Brief Review

The DNA mismatch repair (MMR) system is necessary for the maintenance of genomic stability. The MMR system promotes genomic fidelity by repairing base-base mismatches, insertion-deletion loops (IDLs) and heterologies generated during DNA replication and recombination.
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Salmonella Serovars and Their Host Specificity

Salmonella is a causative agent for a wide variety of pathological diseases in humans, cattle, poultry and other farm animals and hence Salmonella infections are a major cause of concern to humans, veterinary animals and to food industry.
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Editorial Board Members Related to antigen

Eva Mischak-Weissinger

Professor
Department of Hematology, Hemostasis, Oncology and Stem Cell Transplantation
Hannover Medical School
Germany

ASIF ALI

Assistant Professor
Academy of Scientific & Innovative Research (AcSIR)
India

KRZYSZTOF KOSTRO

Professor
Department of Epizootiology and Clinic of Infectious Diseases
Lublin University
Poland

Sameh S.M. Soliman

Assistant Professor
Department of Medicinal Chemistry
College of pharmacy
University of Sharjah
UAE

Jose V. Torres

Professor
Department of Medical Microbiology and Immunology
School of Medicine
University of California at Davis
United States
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