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Articles Related to assay

Impact of the Biofield Energy Healing Based Test Formulation on Various Health Biomarkers Using Cell-Based Assays

Various complementary approaches have been used against multiple organ dysfunction syndrome (MODS), which is the major contributor in high mortality among the healthcare centers.
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Optimization of a Conventional PCR Assay for the Identification of Corynebacterium Pseudotuberculosis from Pyogenic Lesions

Caseous lymphadenitis (CL) is a pyogenic disease that is caused by zoonotic bacteria and is in the same family as Corynebacterium diphtheriae (the etiologic agent of human Diphtheria).
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Occurrences of Dairy Calf Mortality and Morbidity and the Associated Risk Factors in Sululta and its Environs, Central Ethiopia

Calf morbidity and mortality are important causes of economic losses on dairy farms worldwide. A cross-sectional study and clinical observation was conducted from November 2016 to April 2017 with the objective of determining calf morbidity and mortality and to investigate the potential risk factors for mortality and morbidity in Sululta and its environs. A total of 312 respondents engaged in market oriented small holder dairying were interviewed using a structured questionnaire survey about their farm and calf management practices and major calf health problems encountered and diseases that causes mortality. The overall magnitude of morbidity and mortality of calves were 31.0% and 58.37%, respectively. The major calf diseases found were diarrhea (69.34%), pneumonia (16.54%), liver fluke (2.4%), bloat (2.0%), joint ill (2.4%) and other cases (8.04%). Risk factors such as weaning age, breed and awareness of colostrums, feeding of calf and overall farm management were included. In this study 80.3% of calf mortality occurs under age 3 month and 19.42% is above 3 month. Based on laboratory examination, Salmonella and E.coli were detected from diarrheic calves. Salmonella found at rate of 2/29 (3.6%) and E.coli found at only genus level. In conclusion, the magnitude of calf morbidity and mortality found in this study were much higher than economically tolerable level and could greatly affect the productivity of the dairy farms through mainly decreasing the availability of replacement stock and production of milk. It is therefore, suggested that implementation of improved calf and farm management practices and proper environmental protection in the study areas would significantly reduce calf mortality and morbidity.
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Caenorhabditis elegans (C. elegans) Used as a Whole Model Organism to Identify New Anti-Infectives Therapeutic Agent for MRSA Pathogens - A Review Paper

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the greatest fears with the number of serious infections on human health regarding antibiotic resistance. It causes a wide range of infections and bacteremia, ranging from inconsequential superficial skin infections, wound suppuration, even pneumonia or deep-seated tissue infections, which may lead to sepsis and fatalities.
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Two Novel Cases of Marfan Syndrome with FBN1 whole Gene Deletion: Laboratory Assay and Cases Review

Background: Marfan syndrome is a hereditary disorder of connective tissue mainly characterized by abnormalities in the cardiovascular, skeletal and ocular systems. It is most frequently due to heterozygous point mutations in the FBN1 gene located on chromosome 15q21.1 and coding for fibrillin-1 protein. Over 3000 FBN1 mutations have been reported in the FBN1 Universal Mutation Database, among these FBN1 large rearrangements account for <2%. Several studies have shown that 15q deletions encompassing the whole FBN1 gene are associated with Marfan syndrome and FBN1 haploinsufficiency is involved in the pathogenesis of the disease. Since now 19 cases of FBN1 entire deletion have been already described.
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Field Evaluation and Use of a Non Commercial Peptide Enzyme Immunoassay for Human Immunodeficiency Virus Serotyping in Abidjan (Ivory Coast)

In West Africa, where Human Immunodeficiency Viruses 1 and 2 (HIV-1 and HIV-2) co-circulate, serological assays allowing the reliable serotyping of HIV infection are needed.
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Isolation, Identification, and Characterization of the Novel Antibacterial Agent Methoxyphenyl-Oxime from Streptomyces pratensis QUBC97 Isolate

Streptomyces species are medically, industrially, and microbiologically important bacteria that are found in soils and other habitats. Streptomyces isolates and QUBC97 were cultured on a new medium (CCG). Agar plug diffusion test showed antibacterial activity. N-butanol extracts analysed by silica thin layer chromatography (TLC), HPLC purified and identified by MS analysis. QUBC97 was identified based on morphology, PCR, and DNA sequencing of two loci.
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Congenital Dyserythropoietic Anemia Type III and Primary Hemochromatosis; Coexistence of Mutations in KIF23 and HFE

Congenital dyserythropoietic anemia type III (CDA III) can be caused by mutation in KIF23. CDA III differs from CDA I and II in the sense that secondary hemochromatosis has not been reported. However, we have observed elevated serum ferritin in a CDA III family.
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DNA Methylation Status of the Methylenetetrahydrofolate Reductase Gene is associated with Depressive Symptoms in Japanese Workers: A Cross-Sectional Study

Polymorphisms in methylenetetrahydrofolate reductase (MTHFR) gene, that is considered to be the most important genetic determinant of blood homocysteine concentration, are associated with various diseases, including psychiatric disorders. However, the epigenetic factors influencing on the transcription and expression of this gene are unclear.
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Simultaneous HPLC Assay of Paracetamol and Sulfapyridine as Markers for Estimating Gastrointestinal Transit of Amphotericin B-containing Nanoparticles in Rat Plasma

A simple and sensitive two-step reversed-phase HPLC method was developed and validated for determining amphotericin B, paracetamol and sulfapyridine in rat plasma using piroxicam as internal standard.
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Human Induced Pluripotent Stem Cells Derived from A Patient with Sporadic Alzheimer’s Disease Exhibit Altered Neuronal Proliferative Capacity

We generated two hiPSC clones from a patient with sporadic late-onset Alzheimer’s disease (AD-iPSCs), which expressed typical undifferentiated markers and passed standard pluripotency assays. Genome-wide microarray analysis revealed that AD-iPSCs were highly similar to control hiPSCs and hESCs, albeit with some noticeable differences in few genes, viz.: DNAJC15, GRPR, NAIP and SNORD116-18
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Proteomics in Diagnosis: Past, Present and Future

In the post genomic era when several proteomes are on the verge of completion, promising field of protein based diagnostic techniques is emerging. Although protein detection have been used for a long time in clinical diagnostic test, yet high throughput proteomics approaches along with systems biology could be a step forward, towards the development of next generation diagnostic tools and pave a way for personalized medicine.
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Increased High Mobility Group Protein A2/SMAD3 Relates to Ovarian Cancer Progression

The high mortality associated with ovarian cancer is generally related to the development of drug-resistant disease. HMGA2 protein, a member of the high-mobility group AT-hook (HMGA) family of non-histone chromatin binding factors, is overexpressed in high-grade serous ovarian and tubal carcinomas, though little is known about its contribution to disease progression and drug resistance. We sought to assess whether compositional changes in HMGA2 production were associated with ovarian cancer progression.
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Isolated Laryngeal Coccidioidomycosis - A Case Report and Review of the Literature

Coccidioidomycosis involving the larynx is a rare occurrence which seems to present with non-specific upper airway symptoms. The diagnosis is made by tissue biopsy and the treatment is usually fluconazole for 6-12 months depending on the clinical presentation. In unusual presentations of laryngeal problems clinicians should consider Coccidioidomycosis, especially in endemic regions.
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Short-term Creatine Supplementation Suppresses the Cortisol Response to a High-Intensity Swim-Sprint Workout

The primary aim of the present study was to evaluate the effects of creatine ingestion on the metabolic hormone cortisol in male swimmers. Seventeen male swimmers (24.5 ± 3.9 years) with at least 5 years of competitive swimming experience and engaged in swimming training at least 4 times per week participated in the study. Subjects supplemented with creatine (20 g/day) + maltodextrin (1g/kg/day) or maltodextrin (1g/kg/day) only for 6 days prior to a progressive swim-sprint workout. The swim-sprint workout consisted of 8 progressive 100 meter freestyle sets starting at 65% of maximal intensity leading up to a 100% maximum capacity set on the eighth and final set.
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Editorial Board Members Related to assay

M.M. Saxena

President (Vice Chancellor)
Tantia University
India

Yu Shyr

Professor
Department of Biomedical Informatics
Vanderbilt University
United States

Zafar K. Khan

Professor
Department of Microbiology and Immunology
Drexel University
United States

Prakash Prajapat

Assistant Professor
Department of Chemistry
Mehsana Urban Institute of Sciences
Ganpat University
Gujarat.

Baolin Zhang

Division of Therapeutic Proteins
Office of Biotechnology Products
FDA/Center for Drug Evaluation and Research
United States

Maria Amália da Silva Jurado

Assistant Professor
Department of Life Sciences
University of Coimbra
Portugal

Scott Asbill

Professor of Pharmaceutics
College of Pharmacy & Health Sciences
Campbell University
United States

Dong-Hua Yang

Assistant Professor
Fox Chase Cancer Center
United States

Janet Liversidge

Professor
School of Medicine and Dentistry
University of Aberdeen Institute of Medical Sciences
United Kingdom

NAZMUL HOQUE

Assistant Professor
Department of Gynecology, Obstetrics and Reproductive Health
Bangabandhu Sheikh Mujibur Rahman Agricultural University
Bangladesh
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