Articles Related to autologous
Evaluation of The Viability and Phenotipe of Adipose Derived Cells Harvested Using Different Harvesting and Processing Procedures: A Pilot Study
Abstract
Background: Clinical studies demonstrated the efficacy of therapies based on the autologous grafting of adult mesenchymal stem cells to accelerate the healing and regenerative processes of the skin and mesenchymal tissues therefore, this is
considered a valuable approach in the aesthetic rejuvenation treatment to give volume restoration and skin regeneration
effects.
Objective: The aim of this project consists of the evaluation of the cell viability of adipose tissue (AT) harvested using
the Superficial Enhanced Fluid Fat Injection (SEFFI) procedure standardized by the Authors (AG, FPB). The harvesting
procedure was performed using two different cannulas having 0.8 mm and 1 mm side-port holes, respectively. Cells phenotype and ability to adhere to the plastic surface have been analysed. The results have been compared to those recorded
in adipose tissue harvested using a liposuction system and processed with enzymatic digestion (collagenase).
Methods: This study was performed on adipose tissues harvested from 7 patients (6 females and 1 male) with an average
age of 48.5 years with two different techniques and three different cannulas. We compared the cell vitality of every sample
at T0 and T72. Moreover, the samples were analysed to determine the phenotype: cells were incubated with antibodies
anti human against CD90-FITC, CD73-PeCy7, CD44-PE, CD31-PECy5, CD235a- PECy7, CD34-FITC, CD45-FITC and
CD146-FITC and read using the flow cytometer s3e Cell Sorter, BioRad. Results were analysed using the software Flow Jo.
A Case of Lambda AL Type Skeletal Amyloidosis with Vascular Myopathy Achieved Clinical Response after Autologous Stem Cell Transplant
Amyloidosis involving the vascular musculoskeletal system is rare and currently, there is no standard treatment protocol for the treatment
of skeletal muscle AL amyloidosis associated with vascular myopathies. Furthermore, the data on the efficacy and safety of autologous
stem cell transplant is unclear. In this rare case, a biopsy-proven diagnosis of AL type amyloidosis with an unusual presentation of
proximal muscle weakness significantly improved with high dose melphalan followed by autologous stem cell transplantation. The
patient achieved and maintained remission for a year post-transplant
Rehabilitation Providers’ Perspective on Recovery Following Autologous Chondrocyte Implantation: A Qualitative Study
Autologous Chondrocyte Implantation involves a lengthy recovery process. Rehabilitation providers have an opportunity to positively influence outcomes. To explore and describe the experiences of rehabilitation providers’ experiences during the rehabilitation process following autologous chondrocyte implantation (ACI) and to determine what strategies they employ to improve outcomes, encourage rehabilitation adherence, and establish positive therapist-patient relationships.
Hirsutism will be not Considered as Sign of Progressive Disease after Autologous Transplant in POEMS Syndrome
POEMS (polyneuropathy, organomegaly, endocrinopathy, M-protein and skin changes) syndrome is a rare multisystem disease
characterized by plasma cell dyscrasia and overproduction of vascular endothelial growth factor (VEGF). Skin changes are usually
multiple cutaneous angiomas, leukonychia, necrotizing vasculitis, cutaneous thickening of sclerodermiform type and hypertrichosis.
VEGF is assumed to be useful in monitoring disease activity, because VEGF levels usually decrease after treatment. We described
the case of a young woman with POEMS syndrome characterized by hirsutism and hypertrichosis who underwent autologous bone
marrow transplantation . Evolutionwas hallmarked by an improvment of radiological signs and biological markers specially VEGF,
while hirsutism didn’t regress.
Human Neutrophils in Patients with Positive Serology for Chagas Disease
Polymorphonuclear (PMN) neutrophil leukocytes are cells of innate immunity recognized in a peripheral blood smear because of their particular multilobed nucleus. Ring shaped nucleous are typical in rats and mice, being described in some pathologies in humans as well as in healthy subjects.
Parenteral Nutrition Utilization in Bone Marrow Transplant Recipients
Bone marrow transplant (BMT) recipients often require parenteral nutrition (PN) to meet their nutrient needs. While general guidelines
for the provision of PN support by nutrition support teams (NSTs) have been shown to decrease inappropriate PN use, recommendations
for nutrition in BMT recipients are lacking.