Articles Related to cardiomyocytes
Penicillin-Streptomycin Induces Mouse Embryonic Stem Cells Differentiation into Cardiomyocytes through MAPK signal pathway
Penicillin and streptomycin are the most commonly used to prevent bacterial infection or contamination in cell culture. However, the role of Penicillin-streptomycin on inducing mouse embryonic stem cells(mESCs) to differentiate into cardiomyocytes is unclear. To illustrate the mechanism of penicillin-streptomycin on inducing mESCs differentiation into cardiomyocytes, we firstly investigated mESCs differentiation into cardiomyocytes by the traditional method. The time- space expressions of cardiac-specific genes were detected by real-time PCR and western blotting at 8 different time-points over a period of 36 days. Furthermore, action potentials were measured by patch clamp for mESC-derived cardiomyocytes. Embryonic bodies through hanging drop method, were incubated with penicillin-streptomycin (P/S) at different concentrations (1×PBS, 1×, 2×, 5×), and then, the number of cardiomyocytes and expression levels of myocardial marker proteins were detected by flow cytometry, cellular immunofluorescence, and western blotting, respectively. We found that the SSEA-1 of mESC presented green fluorescence, and H.E. staining showed karyoplasmic ratio >>1. Besides, TNNI3, TNNT2, ACTN2 and MYL-2v were clearly visible by cellular immunofluorescence. Furthermore, the ACTN2 positive cells rate of 2× and 5× groups were remarkably higher than that in Cntl (1×PBS) group and 1× group (10 Units/mL penicillin; 10 μg/mL streptomycin), as well as the expressions of GATA4, MYL-2v, TNNI3, ACTN2
New Adipocytokines
They are cytokines secreted by adipose tissue considered to be immunomodulating agents, however they can be more accurately put into the larger, growing list of adipose-derived hormones.
Editorial Board Members Related to cardiomyocytes
Jinlian Hua
Professor
Shaanxi Centre of Stem Cells Engineering & Technology
Key Lab for Animal Biotechnology of Agriculture Ministry
Northwest A&F University
China
Shaanxi Centre of Stem Cells Engineering & Technology
Key Lab for Animal Biotechnology of Agriculture Ministry
Northwest A&F University
China
Mohammad Javed Equbal
Department of Biomedical Institute for Regenerative Research
Texas A&M University
USA
Texas A&M University
USA