Articles Related to cytometry
Quantitative Analysis of Candida Cell Wall Components by Flow Cytometry with Triple-Fluorescence Staining
Detailed analysis of dynamic fungal cell wall components is crucial to our understanding of fungal systematics and the biology and physiology of fungal growth. In fungal pathogens this is of particular importance in examining their response to stress. However, current methodologies do not permit fast and accurate or quantitative analysis of cell wall carbohydrate components. Here, we provide a novel method permitting simultaneous quantitative analysis of the major cell wall components of Candida species relying on triple-staining with fluorescent labeling of chitin, β-glucans and mannans.
Improvements to In Vitro Culture Media for Use in Bovine IVF
In assisted reproduction of cattle the design and preparation of in vitro culture media has been instrumental in supporting the development of bovine oocytes and embryos. In vitro production (IVP) involves three main sequential steps: oocyte aspiration and in vitro maturation (IVM); in vitro fertilization (IVF); and in vitro culture (IVC) of early pre-implantation embryos.
Editorial Board Members Related to cytometry
Paulo Nunes Costa Filho
School of Physical Education and Sports, Rio de Janeiro Federal University, Av. Carlos Chagas Filho, 540 - Cidade Universitária, Rio de Janeiro, RJ, 21940-901, Brazil.