Articles Related to immunohistochemistry
Superficial CD34-Positive Fibroblastic Tumor: First Case Report from Latin-America and Review of the Literature
Superficial CD34-positive fibroblastic tumor is an infrequent soft-tissue tumor characterized by a lesion located in the subcutaneous fascia without affecting the soft or deep muscle planes. Its incidence is unknown; therefore, it represents a diagnostic challenge for both the surgical oncologist and pathologist. Given its high risk of local recurrence, extensive surgical resection is the recommended treatment modality. This is the first report of this rare entity in Latin-America.
Gangliocytic Paraganglioma of the Appendix; A Rare Case Report and Review
Gangliocytic paraganglioma (GP) (nonchromaffin paraganglioma, paraganglioma) is a morphologically distinct tumor
most commonly occurring in the second part of the duodenum in the proximity of ampulla of Vater. We herein, present
a case report of appendiceal mass in a 16-year-old male who presented with recurrent right iliac fossa pain and steno sing
cecal tumor on CT abdomen. He underwent right hemicolectomy and histopathological examination of appendix showed
infiltration of appendiceal wall with gangliocytic paraganglioma. To our knowledge this is the first reported case of
gangliocytic paraganglioma of appendix in our area.
Small Cell Variant of Anaplastic Large Cell Lymphoma Presenting As Leukaemia: A Case Report and Review of Literature
Anaplastic large cell lymphoma with a small cell pattern is a rare T-cell lymphoma. This condition is frequently seen in young patients and should be considered in patients presenting with leucocytosis and constitutional symptoms. We report a case of small cell variant (SCV) of ALCL in a 68 year old man diagnosed by ALK immunohistochemistry (IHC) and cytogenetic analysis. The limitations of using only morphology in diagnosing this rare variant, has also been emphasised.
Pathology of Primary Open-Angle Glaucoma: A Multifactorial Disorder. Review of the Literature
A glaucoma is a group of eye conditions that damage the optic nerve, which is vital to good vision and if not detected early eventually
leads to irreversible blindness. Primary open-angle glaucoma the most common type of glaucoma which occurs due to an imbalance
of the eye liquid drainage and production. Although, Primary open-angle glaucoma’s optic neuropathic nature which features retinal
ganglionic cell loss representing the most prevalent form of glaucoma, its exact a etiology and pathogenesis are unknown.
Predictive Value of ERCC1 Expression on Treatment Response to Cisplatin-Based Regimens in Small Cell Lung Cancer: A Retrospective Analysis
We aimed to determine whether the excision repair cross-complementation group 1 (ERCC1) expression predicts response to cisplatin-based chemotherapy in patients with small cell lung cancer (SCLC). This was a retrospective study, in which hospital files of 31 patients (29 males, 2 females; mean age, 62.26±7.71 years) were reviewed. All patients were treated with etoposide+cisplatin. The lung biopsy and mediastinal lymph node samples were applied anti-human monoclonal antibodies against ERCC1: immunohistochemical staining was considered positive if 25-100% of the cells showed nuclear staining, and negative if less than 25% of the cells showed negative or slight staining. Response to treatment was evaluated as regression (complete or partial response), progressive disease, or stable disease. Of the patients, 18 (58.1%) had limited-stage, and 13 (41.9%) had extensive-stage SCLC. Median follow-up duration was 15 months (range: 1-60 months). ERCC1 staining was positive in 9 of 31 patients (29.0%). In 12 patients (38.7%), tumor regression (complete or partial response) was obtained after chemotherapy. Tumor regression rate was higher in ERCC1 positive patients than those with negative ERCC1 expression (66.7% vs. 27.3%, p=0.036). In conclusion, high expression of ERCC1 was associated with higher response rate to cisplatin-based regimens in patients with SCLC.
Immunohistochemical Localization of LH Receptors in Canine Splenic Hemangiosarcoma
Gonad removal has been implicated as a risk factor for the development of canine hemangiosarcoma. With gonad removal, there is a loss of negative feedback to the anterior pituitary, resulting in persistently elevated concentrations of luteinizing hormone (LH). LH receptors can be found in gonadal and extragonadal tissues, including the bladder and skin. The aim of this study was to investigate if LH receptors were expressed in canine splenic hemangiosarcoma. Splenic tumor samples submitted to the Oregon State Veterinary Diagnostic Laboratory from referring veterinarians were with routine immunohistochemical methods. In addition, survival time following hemangiosarcoma diagnosis was obtained from the referring veterinarians. Five of the eight splenic hemangiosarcomas (62.5 %) were positive for LH receptor expression. LH receptor cellular localization in splenic hemangiosarcoma was cytoplasmic and granular, similar to the positive control canine skin sections (Figure 1C). There was no positive staining in the negative controls. LH receptor is expressed in a proportion of HSAs but further study need to be done to assess the significance of this finding.
Immunohistochemical Localization of Ghrelin and IGF-I (Insulin-Like Growth Factor-I) in the Liver and Kidney Tissues of Melatonin-Treated Rats
This study was conducted to investigate the immunohistochemical localization of ghrelin and IGF-I in the liver and kidney tissues in melatonin-treated rats. Rats were divided into three groups as a control, a sham and a treatment groups. While the treatment group received melatonin (10 mg/kg i.p.) for 3 weeks, only ethanol and saline solution were administered to the sham group. The control group received nothing.
Pulmonary Adenocarcinoma Transforming into Small Cell Carcinoma: An Extreme Rarity
Primary small cell lung cancer (SCLC) showing epidermal growth factor receptor (EGFR) mutation is extremely rare. Transformation into SCLC has been reported as an evolution of lung adenocarcinoma acquiring resistance to EGFR tyrosine kinase inhibitors (TKI) and is considered to be a rare resistance mechanism of EGFR-TKI therapy.
Histological and Immunohistochemical Study on the Toxic Effects of Anthracene on the lung and liver of Adult Male Albino Rats and the Possible Protective Role of Ocimum gratissimum Extract
Anthracene is the simplest tricyclic aromatic hydrocarbon. It has been identified in surface and drinking water, ambient air; exhaust emissions, smoke of cigarettes and cigars and in smoked foods and edible aquatic organisms. It is primarily used as an intermediate in the production of dyes.
Fangchinoline Inhibits Breast Tumor Proliferation and Induces Apoptosis in MDA-MB-231 Cell Line in Vivo
Immunohistochemistry (IHC) was used to determine the protein levels of p27, CD117 and ki67 in tumor tissue of xenograft. After treatment of Fan, the morphologic changes were observed by DAPI staining, Hoechst staining and TUNEL detection under fluorescent microscopy.
Hepatic Angiosarcoma, going but not gone. Lessons from a single centre experience
Hepatic angiosarcoma is a rare tumour that is often difficult to diagnose. Historically, most cases of hepatic angiosarcoma were seen in the setting of industrial epidemics caused by exposure of workers to toxins such as vinyl chloride. Cases associated with recognised exposure to carcinogens have fortunately been extremely rare for the last three or more decades.
Editorial Board Members Related to immunohistochemistry
Paulo Nunes Costa Filho
School of Physical Education and Sports, Rio de Janeiro Federal University, Av. Carlos Chagas Filho, 540 - Cidade Universitária, Rio de Janeiro, RJ, 21940-901, Brazil.
