Articles Related to peptides

Introduction: Rheumatoid arthritis ( RA)is an autoimmune and inflammatory disease. It is characterized by arthrosynovitis with resultant joint destruction . So, an early clinical diagnosis along with simple serological tests like anti CCP antibodies, C –reactive protein ( CRP) and Rheumatoid factor (RF) play an important role .An attempt was made to study and compare these parameters in OPD and IPD RA patients in our hospital setup, VMMC and Safdarjung Hospital , New Delhi. Aims and Objectives: To study the role of RF , Anti-CCP antibodies and CRP in early detection and prognosis of Rheu- matoid arthritis. Materials and Methods: A Total of 440 samples were studied from January 2018 to December 2020 (3years). Correlation between Rheumatoid factor(RF) ,anti CCP antibodies and CRP in suspected RA patients from various departments like PMR , Orthopaedics , Medicine, Obstetrics and Gynaecology and Paediatrics was attempted. RF and CRP was performed using latex agglutination test and anti CCP antibody by ELISA. Results and Conclusion: Of 440 samples tested, all the factors were positive in 87 patients . Anti CCP and RF alone was seen in 32 patients. RF and CRP positive in 49 patients, RF alone in 22 patients. In 250 patients all the tests were negative. So, Anti CCP along with RF and CRP showed a better positive predictive value in patients of Rheumatoid arthritis. But positivity of these parameters even in patients who lack sign and symptoms also aids in better diagnosis, treatment and prognosis.

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Chikungunya is a viral disease caused by positive sense single stranded RNA virus. High fever, myalgia, arthralgia, body rashes are the characteristic features of Chikungunya transmitted by Aedes mosquitoes. We aim to demonstrate that dominant epitopes of envelope E2 protein can be used for diagnostic purposes as well as vaccine design. Multiple Antigenic Peptide (MAP) approach was used based on B and T cell epitopes of E2 protein. Humoral and cell mediated responses were studied in outbred and inbred H-2d mice. Different groups of mice were immunized intramuscularly with or without adjuvant (CpG ODN and murabutide) in PLGA microspheres. MAP entrapped in microspheres with CpG ODN showed highest IgG peak titer (2,98,000) with IgG subclass, mostly IgG2a/2b distribution. MAP’s antisera showed significant immunoreactivity with individual peptides. During thymidine incorporation assay for T cells, all the MAPs showed high lympho-proliferative response. Cytokine profile was dominated by Th1 and Th17 response. Thus, all the MAPs showed high humoral and cell mediated response with CpG/ murabutide adjuvant. MAP’s antisera showed significant in vitro neutralization of CHIKV strain in vero cells.

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In China, the scorpion Buthus martensii Karsch is used as functional food and medicinal materials. The scorpion, scorpion venoms and their extracts are effective in treating a variety of nervous system diseases such as epilepsy, apoplexy pains and facial paralysis.

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This study investigated comparative concentrations of individual amino acids, total amino acids (TAA), non-essential amino acids (NEA) and essential amino acids (EAA) in the blood after the administration of Rice Protein Isolate (RPI) compared to Whey Protein Isolate (WPI).

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Heart failure (HF) is a complex clinical syndrome, of a progressive character, that has a limited prognostic factor and manifested by various extracardiac aspects. It represents a serious and growing public health problem worldwide, both for its high prevalence and the severity of its clinical manifestations, being the final common pathway of most diseases. Undernutrition is often associated with HF, especially in the later stages of the disease, and may chronically reach cardiac cachexia, a severe manifestation related to poor clinical prognosis.

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Researchers have been inventing new drugs since time immemorial. Of late it is established that the development of new drugs alone is not sufficient to ensure progress in drug therapy. The missing link is a potential strategy.It involves the apt selection of drug carrier systems.

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The discovery of a substance with antibacterial properties was expected to permanently diminish the occurrence of bacterial infections posing a threat to health and life. Since the discovery of penicillin many new groups of antibiotics have been introduced into treatment of disease in people and animals, but their excessive and frequently unjustified use, and most importantly, incorrect dosage, have led to numerous unanticipated problems in contemporary human and veterinary medicine.

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