Articles Related to rRNA
Molecular Characterization and Resistance Patterns of Multidrug MethicillinResistant Staphylococcus aureus Isolated from Wound
Staphylococcus aureus is a serious risk to public health as it triggers human infections ranges from wound abscess to life threatening states. As involvement to the global effort the objectives of this study were to examine the incidence of Methicillin-resistant Staphylococcus aureus (MRSA) in Taif region, Saudi Arabia. Moreover, this work aimed to estimate the relation between the phenotypic antibiotic susceptibility patterns and the antibiotic resistance genes mong MRSA isolates. A total of 67 wound specimens were taken from patients.
Methicillin-resistant Staphylococcus aureus (MRSA) was selected by growing on Mannitol Salt Agar supplemented with methicillin
(5 mg/l). The prevalence of methicillin-sensitive S. aureus (MSSA) and methicillin-resistant (MRSA) wound carriage among patients
was 51 and 49 %, respectively.
Genetic Characterization and Sequence Variations in 12S rRNA of Mitochondrial Gene in Three Indian Civets (Viverridae) Species: Implication in Wildlife Forensics
Comparative genomics of 12S and 16S rRNAs, cytochrome b (Cyt b) and the control region (CR) of mtDNA genome are commonly used in phylogenetics and wildlife forensics. We document the genetic characteristics and sequence variations of 12S rRNA (384 bp) in Indian civets, viz., the common palm civet (Paradoxurus hermaphroditus) (n=9), small Indian civet (Viverricula indica) (n=7) and Himalayan palm civet (Paguma larvata) (n=5). The nucleotide compositions vary from 17.6% to 36.3%, and found one to two haplotypes in all three civet species. Observed sequence divergence was 0.001 to 0.002 and 0.057 to 0.110 within and between species respectively. The nucleotide diversity was 0.00102 to 0.00184. Tajima’s D value was negative (-0.097256 to -1.36240) but statistically non-significant in all three species. Based on genetic characteristics, we discuss the use of observed forensically informative nucleotide sequencing (FINS) and topology in species identification for forensic purposes among these three civet species.
Assessment of Rumen Microbial Adaptation to Garlic Oil, Carvacrol and Thymol Using the Consecutive Batch Culture System
Although plant derivatives have shown promise in reducing enteric methane (CH4) emissions from ruminants in short-term studies, investigation on possible rumen microbial adaptation to these compounds is still limited. The objective of this study was to assess the possibility of mixed rumen microbial adaptation to antimethanogenic plant derivatives over relatively long-term in vitro incubation. Treatments were: garlic oil, carvacrol and thymol, each at a final concentration of 300 mg/l.