Articles Related to resistance
Transgenic Expression of Sugarcane Mosaic Virus VPg in Maize Inbred Line CML444 Confers Resistance to Maize Lethal Necrosis Disease
Maize is the most important crop in Kenya and parts of Sub-Saharan Africa. However, yields are below potential due to abiotic and biotic constraints. One of the major biotic concerns is maize lethal necrosis disease (MLN), which causes up to
100% yield losses. MLN is caused by the synergistic infection of two viruses, Maize chlorotic mottle virus (MCMV) and a potyvirus, commonly Sugarcane mosaic virus (SCMV)
Genetic Relatedness and Characterization of O25b-B2-ST131, in Stool Isolates of Extended-Spectrum Cephalosporin-Resistant Escherichia Coli Strains in Healthy Children under 10 Years of Age
Background and Purpose: Escherichia coli (E. coli) is one of the multidrug-resistant pathogens, producing extendedspectrum beta-lactamase enzymes. Molecular typing of this pathogen can be useful for determining the source of dissemination and transfer of resistance and virulence genes of these isolates. Considering the significance of infection in children, in this study, we examined the stool flora of children (<10 years) to show the characteristics and clonal relationship of the isolates.
Screening of Carica Papaya x Vasconcellea Cauliflora Hybrids for Resistance to Papaya Ring Spot Virus (PRSV)
Carica papaya x vasconcelleacauliflora and intergeneric F1 hybrids of these species were screened for resistance to severely infected papaya ringspot virus isolates of papaya ringspot virus. Artificial screening for papaya ringspot virus was carried out 27 days after sap inoculation. Out of twenty-nine F1 hybrid plants of CO 7 x Vasconcelleacauliflora, only six plants were found free from PRSV symptoms. Similarly, out of fifty-five F1 hybrid plants of PusaNanha x Vasconcelleacaulifloraonly twenty-three were found free from the symptoms and seventy plants out of 335 plants of CP50 x Vasconcelleacauliflora were found free from PRSV symptoms. The resistance of the hybrids and parents and their hybrids viz, CO 7 x Vasconcelleacauliflora, PusaNanha x Vasconcelleacauliflora and CP50 x Vasconcelleacauliflora were subjected to DAS ELISA test. Molecular marker viz, ISSR markers were used to check and verify the hybridity. ISSR markers showed confirmity on three hybrid progenies viz, CO7V3, CO7V5 and CO7V6 from CO 7 xVasconcelleacauliflora.
Relation between Osteocalcin and Metabolic Profils in Overweight Men: Comparison of Aerobic and Resistance Trainings
Objectives: Recent studies have shown that exercise by affecting bones causes the release of hormones that are effective in
regulating blood glucose, lipid profile and preventing vascular calcification. The present study aims to Comparison effect
of aerobic and resistance exercises on Osteocalcin and Metabolic Profils in Overweight men.
Methods: total of 33 overweight healthy men (BMI 29 = 67.96 ± 0.96 and age 33.2 ± 2.23) were randomly assigned into
three groups: control (n = 11), aerobic (n = 11) and resistance exercise (n = 11) groups. The training program was conducted for 8 weeks, 3 sessions per week for the training groups. Body compositions parameters (weight, body fat percent,
BMI) were analyzed, osteocalcin and blood biochemical parameters were assessed from fasting blood samples before and
after 8-week exercise programme.
Results: Body fat, BMI and body weight reduction following exercise (P<0/05), significantly increased serum Osteocalcin
(P<0/05, baseline vs Post exercise) and blood biochemical parameters significantly changes (P < 0/05). Increase in osteocalcin had a negative correlation with changes in body composition, as well as HOMA-IR and HDL, LDL not significantly
(all P > 0/05).
Conclusion: Aerobic and Resistance exercise cause to changes in body weight, body fat, BMI, as well as the increase level of
osteocalcin and improvement of glucose metabolism and lipid profiles. However, the difference between the two training
groups was not statistically significant.
The Presence of Minority HIV Drug Resistance Variants in The Protease and Gag Regions Confers Poor Response to Therapy Among Subtype A And D Patients
Objective: To determine the prevalence of minority drug resistance variants in the protease and Gag regions among patients failing a protease inhibitor (PI) based regimen with or without a susceptible genotype based on Sanger sequencing technology.
Methods: Samples were obtained from patients who were failing on a protease inhibitor-based regimen (n = 500). Sanger based sequencing was performed as part of the standard of care. Mutation analysis was performed using the Stanford HIV drug Resistance database. A subset of these patient samples was grouped into two categories: those failing a PI based with mutations in the protease region (n = 100) and those failing on a PI based regimen without mutations in the protease region (n = 128). These samples were then analyzed in the protease and Gag regions using Next Generation Sequencing (NGS) technology and analysis of the drug resistance mutations was performed at the 20% and 1% cutoffs.
Results: An initial analysis of the protease region for patients failing with drug resistance mutations revealed that most patients harbored mutations that confer resistance to Lopinavir and Atazanavir, but these mutations had little effect on Darunavir. Furthermore, NGS revealed that in patients failing with and without drug resistance mutations, minority drug resistance mutations were present at each of the drug resistance codons and at codons that confer multi-drug resistance to protease inhibitors. Further analysis of the Gag gene revealed more genetic diversity among patients failing with no mutations in the protease as evidenced by the proportion of polymorphisms at each codon.
Conclusion: Based on Sanger sequencing, a proportion of patients fail a PI based regimen with a susceptible genotype. However, these patients harbor minority variants in the protease and numerous polymorphisms in the Gag region which when combined these could explain their poor response to therapy. Therefore, in order to improve patient care in low resource settings, there is need to adapt NGS as the standard genotyping technique so that minority variants are captured much earlier. In addition, since mutations in the Gag region also play a role in response to PIs, this region should be included in the routine monitoring for response to therapy in patients on a PI based regimen.
Prevalence of Minority Mutations That Confer Multi-Drug Resistance Among Patients Failing a Nucleoside Reverse Transcriptase Inhibitor Based Regimen in Uganda
Background:The extensive use of antiretroviral therapy has favored the emergence of multiple patterns of drug resistance mutations. These mutations evolve over time and are only detected by the conventional Sanger sequencing technology when they exceed 20% at which time there may be cross resistance. Unfortunately, the controversy surrounding the significance of these minority drug resistance mutations is still overwhelming.
Methods: Samples were obtained from patients who were failing on an NRTI based regimen between 2010 and 2019. For the subtype A and D analysis, 1000 patient samples were analyzed while the subtype C sub-analysis was comprised of 363 samples. Sanger based sequencing was performed as part of the standard of care. A subset of these samples was further analyzed using the Next Generation Sequencing (NGS) technology and analysis of the drug resistance mutations was performed at the 20% and 1% cut off to determine the prevalence of minority multi-drug resistant variants.
Results: Although Thymidine analog mutations (TAMs) were prevalent in all subtypes, our analyses showed that these mutations occurred in significantly less proportions among subtype C infections when compared with the subtype A and D counterparts. On the other hand, the Q151M mutation complex was significantly more predominant among patients harboring subtype C variants. Analysis using NGS revealed that minority drug resistant mutations that confer multi-drug resistance (MDR) were present even in patients who exhibited a susceptible genotype based on the Sanger sequencing technique.
Conclusion: Although HIV-1 MDR variants occur in all subtypes, their predominance is subtype specific with TAMs being significantly more predominant among subtype A and D while the Q151M complex more predominant among patients harboring subtype C viruses. Even in patients with a fully susceptible genotype based on Sanger technology, minority variants are present and their evolution to full blown MDR occurs over time such that if their early detection is missed, cross resistance is inevitable.
Study on Prescribing Pattern of Antibiotic used for Urinary Tract Infection in Tertiary Care Teaching Hospital: A Preliminary Antibiotic Stewardship Programme
Urinary tract infection is defined as the presence of microorganism in the urine that cannot be accounted by contamination. First line treatments for UTI are antibiotics. Antibiotic stewardship is the coordinated intervention designed to improve and measure the appropriate use of antibiotic agents, by promoting the selection of optimal antibiotic drug regimen
including dosing, duration of therapy and route of administration.
Molecular Characterization and Resistance Patterns of Multidrug MethicillinResistant Staphylococcus aureus Isolated from Wound
Staphylococcus aureus is a serious risk to public health as it triggers human infections ranges from wound abscess to life threatening states. As involvement to the global effort the objectives of this study were to examine the incidence of Methicillin-resistant Staphylococcus aureus (MRSA) in Taif region, Saudi Arabia. Moreover, this work aimed to estimate the relation between the phenotypic antibiotic susceptibility patterns and the antibiotic resistance genes mong MRSA isolates. A total of 67 wound specimens were taken from patients.
Methicillin-resistant Staphylococcus aureus (MRSA) was selected by growing on Mannitol Salt Agar supplemented with methicillin
(5 mg/l). The prevalence of methicillin-sensitive S. aureus (MSSA) and methicillin-resistant (MRSA) wound carriage among patients
was 51 and 49 %, respectively.
Exploring the Behavioral Drivers of Antibiotic Prescription in Food Animal’s Practitioners
Irrational use of antimicrobials in food animals is reported as a primary cause of antibiotic resistance (AMR) at the animal human interface with detrimental public health implications. Veterinarians are the main player for antimicrobial usage in food producing animals (FPAs). Therefore, this study was aimed to explore the key determinants of antibiotic prescription behavior among FPAs practitioners and its public health implications. For the purpose, a pre-tested questionnaire was presented to FPAs veterinarians.
Epidemiology and Multidrug Resistance of Strongyle Nematodes in Ordos Finewool Sheep
Regular whole-flock treatments and long-term repeated use of similar anthelmintics has led to the development of anthelmintic
resistance and widespread epidemic of sheep nematodiasis. The current work was carried out to understand the prevalence of
gastrointestinal nematodes in Ordos fine-wool sheep and to evaluate the efficacy of currently used anthelmintics.
A Review on How Animals Contribute as a Factor to Antibiotic Resistance
The development and spread of antibiotic resistance have become a major cause of concern. Over a past few decades, no major new
types of antibiotics have been produced and almost all known antibiotics are losing their activity against pathogenic organisms. Overuse and misuse of antimicrobials in animals and humans is contributing to the rising threat of antibiotic resistance as some types of
infections in humans have already developed resistance to most of the antibiotics available for treatment.
Assessment of the Microbiological Quality of the Medico-Technical Equipment, Surfaces, Premises and Staff Hands in the Department of Neonatology of the Departmental University Hospital Center of Ouémé-Plateau in Benin in 2018
Hospital hygiene is a set of preventive measures essential to ensure the quality of care in health facilities
Preparation and Performance Analysis of High Temperature Resistant and High Strength Alcohol Soluble Phosphate/Phenolic Hybrid Adhesive
A kind of alcohol-soluble phosphate adhesive was prepared by using phosphate solution as raw material. A hybrid high temperature and high strength adhesive was prepared by adding phenolic resin. The wetting condition of water-soluble and alcohol-soluble phosphate adhesive on the surface of hydrophobic material, the composition and mechanical properties of the adhesive were analyzed by means of liquid drop shape analyzer (DSA), X-ray diffraction instrument and universal tensile force machine. The results show that the
alcohol-soluble phosphate adhesive has good wetting effect on hydrophobic materials and has excellent high temperature resistance and mechanical properties.
Metabolic Effects of an Inositol-Resveratrol Nutraceutical Combination in Non-Diabetic Overweight/Obese Subjects with Altered Glucose Tolerance
Insulin resistance (IR) is almost constistently associated with overweight or obesity in both diabetic and non-diabetic subjects.
Nutraceutical compounds improving insulin resistance (IR) may be useful in prevention and treatment of cardiovascular (CV) risk factors in metabolic diseases.
In Vitro Antimicrobial Susceptibility of Brucella Species Isolated from Human and Animals in India
Brucellosis is a zoonotic disease that affects a wide range of animals, including domestic livestock, and human. It is considered one of the most common bacterial zoonoses in the world. Endemic in many parts of India little is known about the antibiotic susceptibility or resistance of Brucella isolates from India.
Anodizing for Design and Function
Two basic reactions occur during the anodizing of aluminum: 1) the aluminum is consumed and 2) an oxide grows. By accepting this statement as true, the anodizing process can be viewed as a corrosion process, and anodizing can be modeled using the Tafel Equation. Anodizing process parameters of electrolyte chemistry and concentration, temperature, aluminum substrate resistance and current density are presented as they relate to the Tafel Equation and how they impact the anodic aluminum oxide structure and properties. Understanding this relationship is consequent in making anodizing an engineering process, one that enables tuning the structure such that it yields distinct characteristics to fulfill design and application requirements.
Editorial Board Members Related to resistance
PREET M. CHAUDHARY
Professor
Department of Medicine
University of Southern California
United States
Department of Medicine
University of Southern California
United States
Dong-Hua Yang
Assistant Professor
Fox Chase Cancer Center
United States
Fox Chase Cancer Center
United States
ELENA A. USACHEVA
Assistant Professor
Department of Pathology
University of Chicago Pritzker School of Medicine
United States
Department of Pathology
University of Chicago Pritzker School of Medicine
United States
AMY H. TANG
Associate Professor
Department of Microbiology and Molecular Cell Biology
Eastern Virginia Medical School
United States
Department of Microbiology and Molecular Cell Biology
Eastern Virginia Medical School
United States
Rula M. Darwish
Professor
Department of Pharmaceutical Microbiology
University of Jordan
Jordan
Department of Pharmaceutical Microbiology
University of Jordan
Jordan
Steve A. Maxwell
Associate Professor
Department of Molecular and Cellular Medicine
Texas A&M Health Science Center
United States
Department of Molecular and Cellular Medicine
Texas A&M Health Science Center
United States
Ming Tan
Associate Professor
Mitchell Cancer Institute
United States
Mitchell Cancer Institute
United States
CARMEN CASTANEDA SCEPPA
Associate Professor
Department of Health Sciences
Bouvé College of Health Sciences
Northeastern University
United States
Department of Health Sciences
Bouvé College of Health Sciences
Northeastern University
United States
LUIS CLAUDIO NASCIMENTO DA SILVA
Department of Parasitary Biology
Ceuma University
Rua dos Castanheiros Jardim Renascença 65075120 - São Luís
Brazil
Ceuma University
Rua dos Castanheiros Jardim Renascença 65075120 - São Luís
Brazil
Yosra A. Mohamed
Research Associate
Department of Veterinary Preventive Medicine
The Ohio State University
USA
Department of Veterinary Preventive Medicine
The Ohio State University
USA