Formulation and Evalution of Oral Reconstitutable Suspension of Cefpodoxime

Introduction Formulation and Evalution of Oral Reconstitutable Suspension of Cefpodoxime Proxetil Patel GC*1, Prajapati J1, Morthana KM2 and Khunt DM2 1Department of Pharmaceutical Technology, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology (CHARUSAT), Gujarat, India. 2Department of Pharmaceutics, Maliba Pharmacy College, Gujarat, India *Corresponding author: Patel GC, Department of Pharmaceutical Technology, Ramanbhai Patel College of Pharmacy, Charotar University of Science and Technology (CHARUSAT), Changa 388 421, Gujarat, India, E-mail: gayatripatel.ph@charusat.ac.in Citation: Patel GC, Prajapati J, Morthana KM, Khunt DM (2015) Formulation and Evalution of Oral Reconstitutable Suspension of Cefpodoxime Proxetil. J Pharm Drug Devel 3(1): 101. doi: 10.15744/23489782.2.101


Formulation and Evalution of Oral Reconstitutable Suspension of Cefpodoxime Proxetil
ISSN: 2348-9782 Suspension defined as an intimate mixture of dry, finely divided drug with excipients, which, upon the addition of suitable vehicle, yields a suspension [6].Reconstitutable suspension is reconstituted at the time of use and thus can be use as liquid formulation which avoids swallowing problem.Moreover, stability of products for tropical countries is a great challenge as these products are exposed to elevated temperatures (up to 40 °C) and relative humidity (up to 90%) especially during transport and storage.
Ion exchange resins (IER) are solid insoluble high molecular weight polyelectrolyte that can exchange their mobile ions of equal charge with the surrounding medium reversibly and stochiometrically.In IER method, weak cation exchange or weak anion exchange resins are used for taste masking, depending on the nature of drug.Bitter cationic drugs can get adsorbed on to the weak cation exchange resins of carboxylic acid and form the complex which is absolutely tasteless with no after taste, and at the same time, its bioavailability is not affected.Further drug resin complex (DRC) can be formulated as lozenges, chewing gum, suspension or dispersible tablet [7][8][9].The objective of present work was to taste mask Cefpodoxime proxetil using ion exchange resin Kyron T-114 and check the feasibility of incorporating the DRC into reconstitutable suspension to increase patient compliance.

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Percentage yield: Percentage yield of DRC was calculated using following equation.

Evaluation of DRC
Taste Evaluation [11][12][13]: The taste of suspension was checked by panel method.The study protocol was explained and written consent was obtained from volunteers.The optimised DRC and CFPD PRXL were subjected for taste evaluation.Taste evaluation was performed by testing the samples on 6 volunteers in the age group 22-28 years.Each volunteer held DRC equivalent to 25 mg in the mouth for 30 seconds and then spit out.The scale used was (a) 0-Tasteless, (b) 1-Slightly bitter, (c) 2-Bitter, and (d) 3-Very bitter.

Material
Cefpodoxime proxetil was gift sample from Indica Lab.Pvt. Ltd., Ahmadabad.Kyron T-114 was obtained as gift sample from Corel Pharma Chem., Ahmadabad.Xanthun gum, Sucrallose, Methyl paraben and Propyl paraben were obtained as gift sample from Welable Pharmaceuticals.All other chemicals and reagents used were of analytical grade.Deionized distilled water was used throughout study.

Preparation DRC
Weight ratio of Kyron T-114 was placed in a beaker containing required quantity of deionized water and allowed to swell for 2 hrs.Weighed amount of CFPD PRXL was added into it and stirred for 4 hrs.The mixture was filtered and residue was washed with three portions of 75 ml of deionized water and dried.Bound drug in complex was calculated as drug-loading efficiency.DRC was optimized for various parameters like drug to resin ratio, effect of pH, effect of temperature, effect of soaking time of resin and effect of stirring time on taste masking efficiency of drug.

Preparation of oral reconstitutable suspension [10]:
The oral reconstitutable suspension of CFPD PRXL was prepared from the optimized DRC (drug rasin weight ratio 1:3).The formula is presented in Table 1.All the ingredients for suspension were sieved through mesh no.40 to make uniform particle size dispersion.The DRC equivalent to 50 mg/ 5 ml of suspension was mixed with the excipients.The prepared suspension was evaluated for before and after reconstitution parameters.

Ingredients
Functional category Quantity (mg/5 ml) Drug content: DRC equivalent to 100 mg of drug was dissolved in 100 ml 0.1 N Hydrochloric acid in volumetric flask.The mixture was sonicated for 15 min and filtered using Whatman filter paper.The above solution was suitably diluted to get solution of 10 μg/ ml.Drug content was estimated using UV spectrophotometer at 262.5 nm using calibration curve equation.The drug content was measured using following equation, Theoretical concentration of CFPD PRXL = 1000μg/ml Practically Obtained CFPD PRXL Concentration 100

Physical properties of DRC
Physical properties of DRC such as particle size, angle of repose, bulk density, tapped density, compressibility index and Hausner's ratio were determined [14].All parameters were performed in triplicate.
Molecular properties on complexation were studied by Fourier transform infrared spectroscopy (FTIR).Infrared spectra of CFPD PRXL, Kyron T-114 and DRC were obtained using FTIR-8400S, Shimadzu.The pellets were prepared on KBr press, and the spectra were recorded over the wave number 4000 to 400 cm -1 .The spectra were obtained comparatively analyzed.
The samples were analyzed for compatibility by TLC.Stationary phase used was Aluminium backed silica gel 60F254 TLC plate and Mobile phase was Chloroform:Methanol:Toluene (4:2:4 v/v/v).Reference solution was prepared by dissolving 10mg of CFPD PRXL in 100 ml of methanol.Test solution was prepared by DRC equivalent to 10mg CFPD PRXL dissolved in100 ml methanol.
In-vitro Drug release studies were performed using USP type II apparatus (Electrolab).Simulated Salivary Fluid pH 6.8, Water and 0.1 N HCl buffer were used as dissolution media.The volume of dissolution medium was 900 mL and it was maintained at Temperature (37 ± 0.5 °C) and stirred at 50 rpm.10 mL samples were collected at time intervals of 5, 10, 15, 30, minutes.The withdrawal samples were replaced by equal amount of dissolution medium to maintain constant volume.Samples were analyzed by UV Spectrophotometry for determination of CFPD PRXL content using calibration curve equation data.
Dry powder blend, ready for reconstitution was evaluated for physical properties like angle of repose, bulk density, tapped density, compressibility index and Hausner's ratio.
The formulated suspension was evaluated for physical stability by determining the sedimentation volume.Fifty ml of suspension was taken in 100 ml stopped graduated measuring cylinder.The suspension was dispersed thoroughly by moving upside down for three times.Later, the suspension was allowed to settle for three minutes and the volume of sediment was noted.This is the original volume of sediment (H 0 ).The cylinder was kept undisturbed for 14 days.The volume of sediment read at 0 day, on 7th day and on the 14th day was considered as final volume of sediment (H u ).The redispersibility of the suspension was checked by moving the stopered cylinder upside down until there was no sediment at the bottom of the cylinder.

Sedimentation volume= [Eq.3]
The particle size was measured using an optical microscope (Labomed CX RIII, Ambala, India).The slide containing suspension particles was mounted on the stage of the microscope and diameter of at least 100 particles was measured using a calibrated optical micrometer.

Characterization of DRC Thin Layer Chromatography (TLC)
In-vitro Drug release study from DRC Evaluation of oral reconstitutable suspension [15][16][17] After reconstitution, parameters like sedimentation volume and redispersibility of suspension, taste evaluation, particle size, viscosity, pH, drug content and in-vitro drug release study were evaluated.Taste evaluation, drug content and in-vitro drug release study was performed as per method given earlier.
Sedimentation volume and redispersibility of suspension [18,19] H u H 0 Particle size Analysis [18] Viscosity study was carried out using a Brookfield viscometer with spindle no.S61.Viscosity was measured at 100 rpm, at 25 °C.

Determination of Viscosity
pH of the suspension was determined using calibrated digital pH meter.

pH of the suspension
The prepared suspension was subjected to short term stability study for a period of three months as per ICH guidelines.In the present study, stability studies were carried out at 25 °C / 60% RH and 40 °C / 75% RH up to three months.Photostability chamber was used.Physical stability was analyzed by change in appearance and chemical stability was analyzed by the change in the drug content and drug dissolution.

Results and discussion of prepared DRC
As presented in Table 2 the complexation of drug with Kyron T-114 in a weight ratio of 1:3 gave efficient drug loading and taste masking.There was no significant difference in drug loading as well as taste, when drug: resin ratio was changed from 1:3 to 1:5.
The stirring time for all subsequent complexation process was fixed to 4 h.Stirring time between 4 and 5 h showed no significant change.The pH and temperature of solution did not show any significant effect on drug loading.Therefore, pH 7 and room temperature were selected for optimized batch preparation.No significant difference was observed, when soaking time of resin in deionized water was changed from 45 min to 120 min.Thus, the soaking time of resin in deionized water was fixed to 45 min.

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The drug and the DRC of optimized ratio were subjected to taste evaluation.Taste evaluation in volunteers confirmed that the taste of drug was excellent bitter while in optimized DRC the taste of drug was not appeared.
Figures1 demonstrates the drug release studies of CFPD PRXL from the DRC in 0.  In vitro drug release from DRC In 0.1 N HCl more than 80% of drug release was released in 15 min, whereas in simulated salivary pH 6.8 and deionized water, less than 10% drug was released in 15 min.The exchange process of drug release follows Resin --Drug + + X + →Resin --X + + Drug + [Eq.4] Where, X + represents the ions in the GI tract.
The presence of H + ion in the 0.1 N HCl causes displacement of CFPD PRXL, thus facilitating drug release.The amount of drug released was insufficient to impart bitter taste in deionized water and simulated salivary pH 6.8 [20].
FTIR Spectroscopy: The FTIR spectra of pure drug showed characteristic peaks at 1724.24 cm 1 (C= O stretching) together with peaks at 1070.42 cm 1 and 3492.85 cm 1 characterizing C-O stretching of ester group and N-H stretching of amide groups respectively.The peak at 1328.86 cm 1 indicated the presence of aromatic ether in the drug moiety.Significant reduction in the intensity of distinctive peaks of drug demonstrates the formation of complex between drug and the resin molecule (Figure 2).Rf values of drug and DRC were found to be 0.627 and 0.621 respectively.The Rf value of drug in both reference solution and that of complex were nearly same indicating that both CFPD PRXL and Kyron T-114 were compatible with each other in prepared DRC (Figure 3).The ultimate height of the solid phase after settling depends on the concentration of solid and the particle size.In prepared formulation, there was little sedimentation after 7 and 14 days and it could be easily redispersed and gave uniform dispersion after 2-3 stroke.Results are shown in Table 5. Particle size of the particles in suspension was reasonably constant even after 14 days.This indicated no crystal growth.

Particle size analysis of suspension
Sedimentation rate depends on the viscosity of the medium.From sedimentation volume data, it can be seen that suspension is stable and easily redisperse after 14 days.Thus, viscosity of the suspension is sufficient for stability of the suspension.

Conclusion
The reconstitutable blend for suspension was subjected for stability for a period of 14 days.The samples were reconstituted in purified water to formulate a suspension.This was analyzed for pH at 0, 7 and 14 days after reconstitution.There was no appreciable change observed in pH.In the present study, an attempt was made to mask bitter taste of CFPD PRXL by Kyron T-114 (cation exchange resin).Various parameters affecting taste masking like drug: resin ratio, pH, temp, soaking time of resin and stirring time were optimized with efficient loading of drug.The volunteers rated the complexes as tasteless and agreeable.Drug release from DRC in simulated salivary pH 6.8 and in deionized water was insufficient to impart bitter taste.Complete drug release was observed at gastric pH.This approach can be utilized for taste masking of bitter pharmaceutical ingredients leading to improved patient compliance.Taste masked DRC was showed excellent flow properties.Formulated CFPD PRXL reconstitutable suspension has acceptable sedimentation properties.In evaluating period of 14 days no significant change was observed in pH, Viscosity, particle size and drug content.This method is simple and cost effective to prepare taste masked reconstitutable suspension of CFPD PRXL that may be acceptable to the industry.
pH Taste evaluation in volunteers confirmed that the taste of bitter drug in reconstitutable suspension was not appeared.All volunteers found to be tasteless and agreeable of taste of suspension.

Taste evaluation
No significant change was observed in the drug content.

Drug content of the suspension
Results of % drug release are shown in Figure 4.It was observed that resin was not retard the release of drug from suspension.
In vitro drug release

Figure 1 :
Figure 1: In-vitro drug release profile of drug-resin complex in 0.1 N HCl, deionized water and simulated salivary pH 6.8

Figure 3 :
Figure 3: TLC plate showing spots of drug (D) and complex (C)

Figure 4 :
Figure 4: In-vitro Dissolution Profile of reconstituted oral Suspension in 0.1 N HCl

Table 2 :
Optimization of Drug: resin ratioTaste EvaluationTable3presents the results of micromeritic studies for DRC.The Carr's index value between 5-12% indicates excellent compressibility.The values of hausner's ratio less than 1.25% and angle of repose below 30° indicates good flowability of DRC.

Table 3 :
Flow properties of DRC

Table 5 :
Sedimentation study of suspensionPrepared suspension was evaluated for flow properties before reconstitution.Results are shown in Table4.Results showed that reconstitutable blend has an excellent flow properties and optimum drug content value.

Table 4 :
Pre-reconstitution evaluation of dry powder blend

Table 6 :
Evaluation Parameter of after reconstitution oral Suspension