Articles Related to HPLC

Justificative: This trial was conducted in order to register a new generic product of Rivaroxaban. Objective: To evaluate the bioequivalence of rivaroxaban formulations manufactured by Eurofarma Laboratórios S/A and the reference drug, Xarelto (Bayer) under fasting and fed conditions. Methods: Three randomized, open label, balanced, 2 treatments, 4 periods, 2 sequences, single dose, full replicate, crossover studies in 48 healthy adult human subjects under fed and fasting conditions for rivaroxaban 10 mg and 20 mg. Rivaroxaban concentrations in plasma were determined using a validated HPLC-MS/MS method.

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Introduction: Isavuconazole is an antifungal drug used for treating patients with invasive fungal infections. Efficacy and safety of isavuconazole is monitored by measuring plasma isavuconazole concentration using LCMS which is a non-affordable method. We used the HPLC system with a UV detector to measure plasma Isavuconazole concentration. Objective: Improved Reversed-phase high performance liquid chromatography procedure with UV detection is described which is cost effective, simple, precise and easily processed for the measurement of Isavuconazole, a drug used to treat the patients with invasive fungal infections, in blood plasma. Method: The method involves protein precipitation, addition of ammonium dihydrogen phosphate and chromatographic separation on a Hypurity C18 Column using an isocratic mobile phase of acetonitrile and ammonium acetate buffer (pH 8.0, 10 mM) (55:45, v/v). The UV detection was performed at 285 nm. The method provides rapid resolution of Isavuconazole in a 50 uL injection. Result: Lower limit of Quantification (LLOQ) is 0.25 μg/ml in a 50 uL injection volume for Isavuconazole with a recovery consistently > 100 %. The assay is validated over linear range of 0.25 to 10 μg/ml. The intra-assay precision is < 3.53 % and inter-assay is <6.38% relative standard deviation of Isavuconazole. The method demonstrated clean separation, clinically acceptable detection limit and a linear range upto 25 ug/mL. Conclusion: The assay demonstrated applicability in quantifying the drug level and monitoring the therapeutic dose for maintaining effective biological level to have better response in fungal infected patients. The method is cheaper as compared to LC-MS/MS and Tandem Mass spectrometry and the results are reportable on the same day of blood collection.

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The present research deals with the development of a stability indicating reverse phase HPLC with PDA detector method for the determination of Armodafinil Agilent XDB-C18, 150×4.6mm, 5µm or Equivalent column. The present research deals with the development of a stability indicating reverse phase HPLC with PDA detector method for the determination of Armodafinil Agilent XDB-C18,

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Monocrotophos is a extremely hazardous organophosphate insecticide that is extensively used and easily accessible in India.

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BioequivalenceA sensitive ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS) method for measurements of N-butylscopolamine in plasma was developed and validated. A SPE extraction was proposed for the clean up of plasma and N-butylscopolamine-d9 was added as internal standard. The analyses were carried out using a phenyl column and mobile phase of acetonitrile:

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Forensic Scientists are required to identify an ever increasing and more complex assortment of drugs and related compounds.

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Chlorpyrifos is a hazardous organophosphate pesticide used worldwide thus its biodegradation is need of the time. In the present study 13 bacterial strains were isolated from collected soil samples of different agro climatic regions and out of them one bacterial strain was screened out as potent degrader of Chlorpyrifos on the basis of percentage degradation using high pressure liquid chromatography (HPLC).

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France, Greece, Italy, and Spain the major sheep and goat milk producers in Europe, got the initiative through an interim of 30 years (1985 – 2015), to organize - through IDF - 7 symposiums aiming at the upgrade of the Dairy Sector. One major interest was the milk quality. Stepwise, the efforts followed cow milk structural development in organizing the testing laboratory, the definition of quality in composition (fat, protein, lactose, total solids), in Hygienic (Total Bacteria Count, Somatic Cell Count, antibiotics residues and Aflatoxin M1 contamination), adulteration with water and detection of milk species. EU, by Decision 2002/657/EC, defined the rules for the Directive 96/23/EC, enumerated the testing methods, the demands in sampling procedures, the minimum limits of performance, the quality control (ISO 17025) and the interpretation of results. The equivalency of results from different laboratories, through ISO 17025, demanded the application of ISO analytical standards.

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Hemoglobin A2 (Hb A2) is a minor component of the hemoglobin present in normal adult red blood cells, accounting for 1.5-3.5% of the total hemoglobin in healthy individuals.

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Haemoglobinopathies are inherited disorders of haemoglobin synthesis that are responsible for significant morbidity and mortality all over the world.

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Sickle cell disease (SCD) is a hemoglobinopathy. Based on genotypes, it is classified into sickle cell thalassemia (SCTh) and sickle cell anemia (SCA).

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Pregabalin is a ligand for the alpha-2-delta subunit of voltage-gated calcium channels in the central nervous system. It has anticonvulsant, analgesic, and anxiolytic activity. The bioequivalence of a test formulation was evaluated with respect to its corresponding reference drug formulation of oral pregabalin 300 mg, administered as a capsule. This randomized-sequence, single-dose, single-blind, two-period crossover design under fasting conditions was done on 25 healthy Mexican adult subjects including both genders. There was a seven-day washout period.

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A simple and sensitive two-step reversed-phase HPLC method was developed and validated for determining amphotericin B, paracetamol and sulfapyridine in rat plasma using piroxicam as internal standard.

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Eugenol (EU) and ibuprofene (IBU) were covalently bound to a bi-functionalized PEG, used as molecular carrier of drugs and the release kinetics of the two bioactive molecules was studied in vitro in buffer solution at pH 7.4, in simulated gastric fluid and in mouse plasma.

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A new, stable and accurate high performance liquid chromatography ultraviolet spectrophotometric method for quantitation of Ciprofloxacin in human plasma was developed and validated.

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