Articles Related to Proteomics

Immunodepletion of high-abundant proteins (HAPs) aids in the identification and analysis of low-abundant proteins (LAPs) in complex samples. Currently, immunodepletion methods for bovine samples are very limited whereas greater availability exists for human and murine animal samples. In this study, we report the simultaneous depletion of HAPs from bovine samples using an immunoaffinity depletion cartridge (the multiple affinity removal system, MARS) designed to target six human HAPs, while such a depletion kit for bovine samples is not available.

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Treatment of Hepatitis B infection is available with the help of existing drugs but the eradication of HBV infection is still under the pipeline. The application of novel drugs and gene-based therapies for HBV infection in need of the hour, however, the lack of efficient cell culture system, animal models for viral infection and replication acts as a major obstacle for novel therapies. This not only hampers the progress of HBV research but also stress the need for effective forthcoming therapeutics for Hepatitis B infection. Proteomic studies to understand the protein ubiquitination and to find molecular markers are the effective tools to study the HBV infection, pathogenesis and its control.

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1-D, 2-D gel electrophoresis, mass spectrometry and bioinformatics analysis were established and utilized to examine the changes in protein expressions associated with germination of Ceiba pentandra seeds.The change in protein pattern was analysed by 1-D and 2-D gel electrophoresis for better separation and to ascertain the pattern of protein mobilization during germination.

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We hypothesized that quantitative tandem mass spectrometry-based proteomics, incorporating rapid microwave and magnetic sample preparation (M2 proteomics), might enable relative protein expression to be correlated to childhood B-precursor acute lymphoblastic leukemia (B-ALL) cytogenetic subtypes, corresponding to low-risk (ETV6-RUNX1) and high-risk (MLL-R) subtypes.

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In the post genomic era when several proteomes are on the verge of completion, promising field of protein based diagnostic techniques is emerging. Although protein detection have been used for a long time in clinical diagnostic test, yet high throughput proteomics approaches along with systems biology could be a step forward, towards the development of next generation diagnostic tools and pave a way for personalized medicine.

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