Articles Related to plasma
Reversed-Phase High Performance Liquid Chromatography Procedure for the Determination of Isavuconazole in Human Plasma
Introduction: Isavuconazole is an antifungal drug used for treating patients with invasive fungal infections. Efficacy and safety of isavuconazole is monitored by measuring plasma isavuconazole concentration using LCMS which is a non-affordable method. We used the HPLC system with a UV detector to measure plasma Isavuconazole concentration.
Objective: Improved Reversed-phase high performance liquid chromatography procedure with UV detection is described which is cost effective, simple, precise and easily processed for the measurement of Isavuconazole, a drug used to treat the patients with invasive fungal infections, in blood plasma. Method: The method involves protein precipitation, addition of ammonium dihydrogen phosphate and chromatographic separation on a Hypurity C18 Column using an isocratic mobile phase of acetonitrile and ammonium acetate buffer (pH 8.0, 10 mM) (55:45, v/v). The UV detection was performed at 285 nm. The method provides rapid resolution of Isavuconazole in a 50 uL injection.
Result: Lower limit of Quantification (LLOQ) is 0.25 μg/ml in a 50 uL injection volume for Isavuconazole with a recovery consistently > 100 %. The assay is validated over linear range of 0.25 to 10 μg/ml. The intra-assay precision is < 3.53 % and inter-assay is <6.38% relative standard deviation of Isavuconazole. The method demonstrated clean separation, clinically acceptable detection limit and a linear range upto 25 ug/mL.
Conclusion: The assay demonstrated applicability in quantifying the drug level and monitoring the therapeutic dose for maintaining effective biological level to have better response in fungal infected patients. The method is cheaper as compared to LC-MS/MS and Tandem Mass spectrometry and the results are reportable on the same day of blood collection.
An Unexpected Diagnosis of Plasmacytoma of The Thyroid: A Case Report and Review of The Literature
Plasmacytoma is a malignant proliferation of plasma cells that can arise in the bone marrow (osseous) and in the soft tissues. Extramedullary plasmacytomas most commonly occur in the upper respiratory tract. Thyroid involvement is rare. In this report, we describe a new case plasmacytoma of the thyroid gland in a 60-year-old man with a history of multinodular goiter present for several years and an unexpected rapid increase of the mass associated to dysphagia, dyspnea, and dysphonia. Histopathological examination of surgical specimen, along with immunohistochemical studies, unexpectedly led to the diagnosis of plasmacytoma.
We also briefly summarize the last 30 years of literature on thyroid plasmacytoma and review the salient characteristics of this rare condition.
Optical Depth Measurement of Self-Reversed Line Emitted from In-Homogenous Plasma in Laser Induced Breakdown Spectroscopy
In this work, an original method is proposed and used for measurement of the optical depth of a self-reversed spectral line. A theoretical approach is utilized in an in homogenous laser induced plasma at local thermodynamic to simulate self-reversed line and then to get the main line profile without self-absorption. In this research, the spatial and temporal evolution of optical depth of two spectral lines of Ca II at 393.4 nm and 396,9 nm is studied. Furthermore, the variation of optical depth versus the concentration of species emitter inside of plasma plume is investigated. In addition, the validity of this method is affirmed during comparison with results obtained by other techniques.
Plasma Exchange for Neuroborreliosis Associated with Guillain Barre Syndrome
Lyme neuroborreliosis (LNB) is a rare infectious disorder of the nervous system caused by Borrelia burgdorferi spirochetes. Different neurological conditions were reported in the disease.
Blastic Plasmacytoid Dendritic Cell Neoplasm Presenting as a Harbinger of Acute Myeloid Leukemia
A 51 year old female was referred to our dermatology clinic for a rapidly growing lump on head of 6 weeks duration. On examination we observed a 15 cm diameter erythematous crusted tumor on the forehead with significant bilateral cervical lymphadenopathy. An excision biopsy of the tumor revealed a malignant high grade diffuse undifferentiated infiltrating neoplasm involving the skin and subcutaneous tissue.
Amiodarone Prevents Atrial Fibrillation Induced by Arsenic Trioxide in Extramedullary Plasmacytoma Involving Lumbar Disc: A Novel Case Report
Extramedullary plasmacytoma (EMP), characterized by infiltration of malignant plasmablastic clones outside the bone marrow in
absence of multiple myeloma.
Clinicopathological and Prognostic Value of Plasmacytoid subtype in Bladder Cancer: A Systematic Review and Meta-Analysis
The World Health Organization, in 2004, published a new classification that recognizes different histological variants
(HV) of urothelial carcinomas
Determination of N-Butylscopolamine in Human Plasma by Solid-Phase Extraction and UHPLC-ESI-MS/MS: Development, Validation and Application to a Bioequivalence Study
BioequivalenceA sensitive ultra performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS) method for measurements of N-butylscopolamine in plasma was developed and validated. A SPE extraction was proposed for the clean up of plasma and N-butylscopolamine-d9 was added as internal standard. The analyses were carried out using a phenyl column and mobile phase of acetonitrile:
Cattle Ticks and Tick Borne Haemoparasite Species Identification and Associated Risk Factors in Two Districts of West Arsi Zone, Ethiopia
Different tick species and tick-borne diseases of cattle are present in Ethiopia and they induce huge production loss in livestock industry by creating serious animal health problems. The present study was conducted from November 2017 to April 2018 in Arsi Negele and Asasa districts of West Arsi Zone, Oromia National Regional State, Ethiopia. A cross sectional study design and purposive sampling technique were employed to select 384 study animals so as to identify ticks species and tick-haemoparasite species and also to identify risk factors that affect the prevalence and association of tick born diseases. Tick and haemoparasite identification were carried out by using drect stereomicroscopic and direct thin blood examination. The study identified two tick genera and four tick species. The tick species encountered were Rhipicephalus decoloratus (51.6%) Ambylomma variegatum (46.1%) Ambylomma gemma (20.1%) and Rhipicephalus evertsi evertsi (1.2%). Out of all 384 cattle examined for the presence of tick-born haemoparasites 11.4% (n=44) of them were positive. From this 6.2%, 3.6 %, 1.3% and 0.8% of them were positive for Anaplasma marginale, Babesia bigemina, Babesia bovis and Anaplasma marginale, respectively. Two cattle (0.5%) were found positive for both Anaplasma marginale and Babesia bigemina. The highest prevalence of total tick born haemoparasites (57.1%) was occurred in A. Variegatum, A. gemma and R. decoloratus mixed infestations. In conclusion ticks and tick born haemoparasitic infections were common problems in the study area. Thus, awareness creation for farmers about the impacts of ticks and tick born diseases on livestock production and productivity and also applying appropriate and timely strategic tick control methods by veterinary service providers were advisable.
Solitary Plasmacytoma of the Thoracic Spine
Solitary bone plasmacytomas account for approximately 5% of plasma cell malignancies. These lesions are recognized as pre-malignant, if untreated, at least 50% will progress to multiple myeloma within 5 years of diagnosis. We present a case of a 54 year old male patient whom presented to our unit complaining of progressive axial backache complicated by acute onset paraplegia of 8 hours duration. Neurological examination revealed an incomplete L1 sensory level and no motor function of his lower limbs which had power 0/5 in all dermatomes (Frankel B).
Quality of Life after Accidental Major Vascular Injury and Prolonged ICU Stay - A Case Report
Survival or mortality rate is a reasonable choice of an outcome measure for critically ill patients in intensive care unit (ICU). But it is also important to assess the impact of critical illness and peri-operative complications on health status and quality of life (QoL) after hospital discharge. The QLQ-C30 is a 30-item cancer-specific questionnaire that incorporates five functioning scales (physical, role, cognitive, emotional, and social), eight symptoms scales, perceived financial impact of the disease and a global scale [1-5].
Novel Alternative Treatment to Plasmapheresis in Evans Syndrome
Evans syndrome is a rare haematological disease characterised by concurrent occurrence of autoimmune haemolytic anaemia
(AIHA) and idiopathic thrombocytopenic purpura. The autoimmune haemolysis can lead to hyperbilirubinaemia, profound anaemia
and haemodynamic instability. It can be difficult to treat and when refractory to medical treatment it could necessitate emergency
splenectomy. Plasmapheresis is used in refractory haemolytic anaemia to aid removal of disease causing autoimmune complexes whilst
immunomodulators act to reduce autoantibody response and effectiveness. However, this is an expensive and resource intensive process
that is limited to tertiary referral centres. In this case study we present a successful novel alternative for life threatening AIHA in Evans
Syndrome using haemofiltration with on-going fresh frozen plasma transfusion when resources are limited or it is not an accessible
method.
Multidimensional LC/MS-MS Analysis of Synthetic Cannabinoids in Urine, Plasma, and Edibles
Synthetic cannabinoids (SCs) present a multitude of problems in terms of maintaining up-to-date methods of detection. They are novel psychoactive substances originally synthesized for medical use and research purposes. Abuse has demonstrated a variety of effects ranging from euphoria to aggressive behavior and death. The marketing, similar naming, and described pharmacological interactions create the dangerous and false perception that SCs are similar to, or the same as, tetrahydrocannabinol in cannabis products
Development and Optimization of an LC-MS/MS Method for Dosage Form of Ergocalciferol (Vitamin D2 ) in Human Plasma
Accurate measurement of ergocalciferol in biological samples has become easier using high-end mass spectra. Currently, various LCMS/MS methods have been developed to quantify ergocalciferol. Use of LC-MS/MS has accomplished to develop an accurate method that could quantify ergocalciferol in a large number of samples where it should be analyzed in the blood immediately after dosage. The objective of this study is to optimize chromatographic conditions and evaluate the LC-MS/MS method for accurate quantification of the dosage form of ergocalciferol in human plasma. Extraction and separation of ergocalciferol in human plasma was achieved using methanol liquid-liquid extraction and by a reverse phase C8 column, respectively
Evaluation of Apolipoprotein E Fragmentation as a Biomarker for Alzheimer’s Disease
Recent studies have supported a role for the proteolytic cleavage of apolipoprotein E4 (APOE4) as a potential mechanism for the enhanced dementia risk associated with Alzheimer’s disease. To determine whether APOE4 fragmentation is correlated with AD, ELISA assays were performed with cerebral spinal fluid (CSF) and plasma samples utilizing an antibody that specifically detects a 17 kDa amino-terminal fragment (p17) of APOE (nApoECF antibody). In CSF samples, levels of APOE fragmentation were minimal in both neuropathological normals (NPNs) and AD cases and there were no significant differences between the two cohorts across APOE genotypes.
Editorial Board Members Related to plasma

Baolin Zhang
Division of Therapeutic Proteins
Office of Biotechnology Products
FDA/Center for Drug Evaluation and Research
United States
Office of Biotechnology Products
FDA/Center for Drug Evaluation and Research
United States

Suresh G. Joshi
Associate Professor
Department of Microbiology and Immunology
Drexel University
United States
Department of Microbiology and Immunology
Drexel University
United States

RAVINDRA K. MALIK
Professor
Natural and Forensic Sciences Department
Albany State University
United States
Natural and Forensic Sciences Department
Albany State University
United States

Ock K. Chun
Assistant Professor
Department of Nutritional Sciences
University of Connecticut
United States

Joseph Prandota
Emeritus Full Professor
Department of Pediatrics & Clinical Pharmacology
Faculty of Health Sciences
Wroclaw Medical University
Wroclaw
Poland
Department of Pediatrics & Clinical Pharmacology
Faculty of Health Sciences
Wroclaw Medical University
Wroclaw
Poland