Articles Related to Cryopreservation

: Ammonia is corrosive and exposure will result in a chemical-type burn. It is highly hygroscopic and readily transforms the moist areas of the body such as eyes, nose, throat, and moist skin areas. It is a highly toxic irritant gas, and its toxicity usually occurs from occupational exposure, most are unintentional toxicity. It causes tissue damage via exothermic reaction with body tissues causing liquefactive necrosis. Release of ammonia has potentially for harmful effects on workers and the public. Although there have been incidents of exposure to harmful concentrations of ammonia in the world there have been few fatal accidents.Presentation depends on the level and duration of exposure. Management is supportive and its antidote is not available yet

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Therapeutic Hypothermia has been evolving as a science in itself since a very long time. There have been many accidents chronicled wherein people were buried alive in snow or under ice in frigid waters, left scientifically dead without any signs of heartbeat and breathing, only to be revived many hours later. The most notable case being that of Anna Bagenholm from Sweden.

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Development of novel cryopreservation protocols is required to test novel chemical and non-chemical supplements in freezing extender for bee semen. Antioxidants demonstrate critical effects on post-thawed sperm quality, fertility, and subsequent embryo development. The purpose of the study was to determine of the effects of different concentrations of proline, cysteine and glutamine on some sperm quality and fertility of cryopreserved bee spermatozoa.

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With the recent advances in breast cancer treatment the 5 year survival rates have increased significantly with overall 20yrs survival in developed countries like USA. With further improvement being designed in the treatment of breast cancers with more and more sophisticated models to study breast cancer in human beings using the modern microfluidic models one expects more and younger breast cancer survivors to be needing fertility treatments. Here we review the modern advances in breast cancer treatments and the need for emphasizing on fertility preservation before starting any chemotherapeutic or other management as recommended by both ASO and ASRM.

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In assisted reproduction of cattle the design and preparation of in vitro culture media has been instrumental in supporting the development of bovine oocytes and embryos. In vitro production (IVP) involves three main sequential steps: oocyte aspiration and in vitro maturation (IVM); in vitro fertilization (IVF); and in vitro culture (IVC) of early pre-implantation embryos.

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In relation to assisted mammalian reproduction, the goal of cryopreservation is to preserve without significant loss of viability a stock of gametes and/or embryos with a view to thawing those cells for use in in vitro reproduction treatments. There are numerous cryopreservation protocols, which vary in terms of cryoprotectant used, storage temperature, freezing and thawing rates, and the particular cells that they are suitable for preserving.

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Cryopreservation is the use of ultra-low temperatures to preserve whole living cells and tissues in order to retain their structural integrity and maintain their physiological viability. It enables long term storage of cells in order to circumvent the need for continuous in vitro culture. When cryopreserving bovine embryos there are two means of cryopreservation: slow programmable freezing and vitrification. While controlled-rate and slow freezing can be applied widely to in vivo derived-embryos, this methodology remains less successful for embryos produced in vitro.

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