Articles Related to ITO

Justificative: This trial was conducted in order to register a new generic product of Rivaroxaban. Objective: To evaluate the bioequivalence of rivaroxaban formulations manufactured by Eurofarma Laboratórios S/A and the reference drug, Xarelto (Bayer) under fasting and fed conditions. Methods: Three randomized, open label, balanced, 2 treatments, 4 periods, 2 sequences, single dose, full replicate, crossover studies in 48 healthy adult human subjects under fed and fasting conditions for rivaroxaban 10 mg and 20 mg. Rivaroxaban concentrations in plasma were determined using a validated HPLC-MS/MS method.

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Pyoderma gangrenosum is a rare ulcerating skin disease often presenting as an extra intestinal manifestation of IBD and is difficult to manage. Treatment options for pyoderma gangrenosum include steroids, calcineurin inhibitors and anti TNF agents. Here in, we report a case of recurrent Pyoderma gangrenosum with Ulcerative Colitis that was successfully managed with Tofacintib, a JAK inhibitor

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Dengue fever remains an annual worldwide threat to human health, even though Aedes eradication programs based on the typical route of mosquito- borne transmission have successfully controlled dengue epidemics to a considerable extent. Interestingly, most cases of dengue infection in humans reported in the tropical regions of Asia, Oceania, Africa, and the Americas are due to horizontal transmission. This suggests contribution of atypical transmission routes to continuous infections.

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Immune-checkpoint inhibitors (ICI) have provided groundbreaking advancements for a variety of malignancies. It has been of recent interest to identify predictive indicators of response to improve cancer management using immunotherapy. The intestinal microbiome has been recognized as a potential predictor of ICI anti- tumor activity. Antibiotics reduce diversity the overall composition of the gut microbiota, with effects seen as quickly as in a single day. Post-antibiotic dysbiosis recovery varies depending on type and duration of exposure. Preclinical studies in mice with advanced cancer treated with broad spectrum antibiotics have been associated with resistance to ICI treatment.

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Introduction: Isavuconazole is an antifungal drug used for treating patients with invasive fungal infections. Efficacy and safety of isavuconazole is monitored by measuring plasma isavuconazole concentration using LCMS which is a non-affordable method. We used the HPLC system with a UV detector to measure plasma Isavuconazole concentration. Objective: Improved Reversed-phase high performance liquid chromatography procedure with UV detection is described which is cost effective, simple, precise and easily processed for the measurement of Isavuconazole, a drug used to treat the patients with invasive fungal infections, in blood plasma. Method: The method involves protein precipitation, addition of ammonium dihydrogen phosphate and chromatographic separation on a Hypurity C18 Column using an isocratic mobile phase of acetonitrile and ammonium acetate buffer (pH 8.0, 10 mM) (55:45, v/v). The UV detection was performed at 285 nm. The method provides rapid resolution of Isavuconazole in a 50 uL injection. Result: Lower limit of Quantification (LLOQ) is 0.25 μg/ml in a 50 uL injection volume for Isavuconazole with a recovery consistently > 100 %. The assay is validated over linear range of 0.25 to 10 μg/ml. The intra-assay precision is < 3.53 % and inter-assay is <6.38% relative standard deviation of Isavuconazole. The method demonstrated clean separation, clinically acceptable detection limit and a linear range upto 25 ug/mL. Conclusion: The assay demonstrated applicability in quantifying the drug level and monitoring the therapeutic dose for maintaining effective biological level to have better response in fungal infected patients. The method is cheaper as compared to LC-MS/MS and Tandem Mass spectrometry and the results are reportable on the same day of blood collection.

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Many students suffer from the sound of air conditioners and others suffer from light intensity in classroom. The air conditioners and light intensity affect the efficiency and quality of education. Urduino is used a lot in industrial areas because there is a lot of noise pollution. Additionally, it can be used in high traffic regions as well as to monitor noise in different parts of the city. It can also be used in education systems to monitor the noise in classrooms. The purpose of this study was to monitor the noise in classrooms by Arduino Nano that interfaced with a Sound Sensor. A study was conducted to determine the ambient sound and light intensity of a convenience sample at school during September, October and November 2021. Six classrooms’ samples corresponding to hourly fractions of the average sample broken down into the following periods: from 10:00 am to 2:50 pm. Sound levels were measured to assess acoustic conditions. Light levels were measured to assess light conditions. Each classroom from 1 to 6 was tested the ambient sound intensity and light using an Arduino. Noise varies with time and place. The sound level varies between 27.5 dB and 65 dB with the average sound level in the classrooms was 43.53 dB greater than recommended sound level limit of 35 dB. The light intensity varies between 62.50 lux and 208.5 lux. The average light levels around the recommended 300 lux. Everyone emphasized the quality of using the device to measure the noise level in the classroom. The use of the device leads to monitor the noise of the air conditioner or replacing it. The device has the potential to significantly change the noise pollution monitoring system at school. Researchers recommend all students and authorities the importance of the Arduino.

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Background:The extensive use of antiretroviral therapy has favored the emergence of multiple patterns of drug resistance mutations. These mutations evolve over time and are only detected by the conventional Sanger sequencing technology when they exceed 20% at which time there may be cross resistance. Unfortunately, the controversy surrounding the significance of these minority drug resistance mutations is still overwhelming. Methods: Samples were obtained from patients who were failing on an NRTI based regimen between 2010 and 2019. For the subtype A and D analysis, 1000 patient samples were analyzed while the subtype C sub-analysis was comprised of 363 samples. Sanger based sequencing was performed as part of the standard of care. A subset of these samples was further analyzed using the Next Generation Sequencing (NGS) technology and analysis of the drug resistance mutations was performed at the 20% and 1% cut off to determine the prevalence of minority multi-drug resistant variants. Results: Although Thymidine analog mutations (TAMs) were prevalent in all subtypes, our analyses showed that these mutations occurred in significantly less proportions among subtype C infections when compared with the subtype A and D counterparts. On the other hand, the Q151M mutation complex was significantly more predominant among patients harboring subtype C variants. Analysis using NGS revealed that minority drug resistant mutations that confer multi-drug resistance (MDR) were present even in patients who exhibited a susceptible genotype based on the Sanger sequencing technique. Conclusion: Although HIV-1 MDR variants occur in all subtypes, their predominance is subtype specific with TAMs being significantly more predominant among subtype A and D while the Q151M complex more predominant among patients harboring subtype C viruses. Even in patients with a fully susceptible genotype based on Sanger technology, minority variants are present and their evolution to full blown MDR occurs over time such that if their early detection is missed, cross resistance is inevitable.

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Primary coenzyme Q10 deficiency-4 (CoQ10D4) is an autosomal recessive disorder characterized by childhood-onset of cerebellar ataxia and exercise intolerance. Molecular pathology responsible for clinical findings is mitochondrial respiratory chain dysfunction. The main clinical manifestation involves early-onset exercise intolerance, progressive cerebellar ataxia and movement disorders. Some affected individuals develop seizures and have mild mental impairment, indicating variable severity.

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The collection and disposal of waste is ancient, but has become a crucial issue in a relatively recent time, as far as quantity to recuperate, recovery of resources to deal with, novelty and variety of materials to destroy and disposal techniques at hand. It is known that a consumer society produces an extraordinary quantity of scrap and it is now clear there is urgency of change in disposal management.

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This was a 50-year-old patient with recurrent bilateral nodular anterior scleritis refractory to several treatments, including immunosuppressants and anti Tnfα. She was assessed for the different causes of immune-mediated and infectious diseases, but nothing was found. Tofacitinib was started 5 mg twice a day, which led to the spacing of crises until their total disappearance. At present, the patient has been crisis-free for nine months. In conclusion, the use of Janus Kinase inhibitor (Tofacitinib) alone was effective in the treatment of refractory recurrent nodular anterior scleritis.

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Despite intravenous (IV) catheters being among the most common invasive clinical devices used in a hospital setting, they are associated with complication rates up to 50% in large medical centers. High patient burden combined with enormous costs to the healthcare system necessitates improvement in IV catheter safety and utilization.

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: Aiming to evaluate the recent theoretical postulates on tinnitus underscoring the role of thalamocortical neural tracts, the present study 1) explores Middle Latency Response (MLR) as a possible physiological measure of tinnitus: thus investigates the predicted exaggeration of Pa-Na, Na-Pb interpeak amplitudes in tinnitus patients and 2) explores MLR as a prognostic indicator of Tinnitus Retraining Therapy: thus evaluates possible decrease in Pa-Na & Na-Pb amplitude after 2weeks exposure to Tinnitus Retraining Therapy.

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Present study was planned in order understand the surveillance, browsing behavior and nutritive value of camel browse vegetations. Review of literatures indicated that camel is mostly found at arid and semi-arid areas of the world where average rain fall becomes less than 350 mm per year. The estimated population in the world is 19 million, among which 17 million are supposed to be one-humped dromedary camels (Camelus dromedarius) and 2 million two-humped Bactrian camels (Camelus bactrianus).

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The air pollution’s situation in the Occupied Palestinian Territories (OPT) can be simply described as ‘catastrophic.’

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Insulin resistance (IR) is almost constistently associated with overweight or obesity in both diabetic and non-diabetic subjects. Nutraceutical compounds improving insulin resistance (IR) may be useful in prevention and treatment of cardiovascular (CV) risk factors in metabolic diseases.

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We investigated a method for preparation of ordered nanopore arrays with the interpore distance of 60 nm by guided self-organization of anodic aluminum oxide with a prepatterned array of pits in the starting Al film.

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