Articles Related to Palm Oil
Isolation of Microorganisms Associated with Palm Oil Contaminated Soil
Aim: Palm oil processing generally generates lots of wastewater (palm oil mill effluent), this is usually discharged into
the environment in the untreated form and subsequently causes several environmental issues. There is therefore need to
isolate microorganisms that can be used to clean up the palm oil contaminated environment especially the soil.
Methods and Results: Palm oil contaminated soil was obtained from Oba Adeyemi palm oil mill in Oyo, Oyo State, Nigeria,
other soil samples which were purposely contaminated with palm oil, were obtained from Ajayi Crowther University
Oyo, Oyo State. Isolation, characterization and identification of microorganisms were carried out using morphological
and biochemical characterization. The isolates were preliminarily screened for lipolytic activities, this was confirmed
by growth on the mineral salt medium after 7 days, signifying hydrolysis. One of the prominent isolates was further
identified by sequences analysis of 16S rRNA genes. Forty-one bacterial isolates were identified, which included species of
Bacillus (80 %), Pseudomonas (20 %) in the oil mill contaminated soil sample and Bacillus spp. (100 %) in the purposely
contaminated soils. Twenty-nine fungal isolates including species of Aspergillus, Oidiodendron, Geotrichum, Penicillum,
Saccharomyces were isolated with Aspergillus fumigatus having the highest frequency of occurrence (37.5 %) in artificially
contaminated soil and Saccharomyces spp. having the highest frequency of occurrence (91 %) in palm oil contaminated
soil from the palm oil mill. Sequencing of the 16S rRNA of one of the prominent isolates showed that it was identified as
MN607220 Saccharomyces cerevisae. All the bacterial and fungal isolates had lipolytic activities except Bacillus mycoides
and Oidiodendron sp. respectively. Nine of the ten Saccharomyces sp. had lipolytic activities.
Conclusion: These screened organisms could therefore be employed for the cleanup of palm oil contamination in the
environment.
Significance and Impact of Study: Thereby ridding the environment of possible toxic effects especially in areas of need
like Malaysia