Articles Related to Quantification

To adapt the perfusion-CT (PCT) examination-protocol in patients with liver cirrhosis and suspected hepatocellular carcinoma (HCC) while keeping diagnostic accuracy and qualitative assessment of tumor enhancement patterns (wash-in/wash-out) at comparable radiation dose.

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A simple High-Performance Thin layer chromatography (HPTLC) method has been developed for determination of atenolol in human blood and viscera. The mobile phase was ethyl acetate, acetone, ethyl alcohol, ammonia solution in the ratio of 45:45:7:3. The densitometry scanning at 254 nm was found to be maximum absorption for standard atenolol. The standard and extracted atenolol from samples were detected by HPTLC and confirmed with authenticated spectra at 254 nm. The quantity of the atenolol determined to be 1.6 µg for viscera and 1.5 µg for blood sample.

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The CIGB-552 peptide is a synthetic peptide that exerts cytotoxic effect on tumor cells. Thus, CIGB-552 peptide quantification in patient samples is crucial for assessing the treatment efficacy. Therefore, this study describes the generation and characterization of monoclonal antibodies (mAb) directed against the CIGB-552 peptide to be used in a quantification assay in further pharmacokinetic studies in humans. In this sense, the CIGB.552-H294 mAb specificity was evidenced only against the CIGB-552 peptide and a metabolite of 17 amino acids resulting from the CIGB-552 peptide degradation detected in mouse sera.

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A new, stable and accurate high performance liquid chromatography ultraviolet spectrophotometric method for quantitation of Ciprofloxacin in human plasma was developed and validated.

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Capsaicin, an active ingredient of Capsicum fruit, is currently undergoing “revival” in the clinical management of pain. However, the choice of its formulation is rather limited to the use of “old-fashioned” tinctures and recently the patches. In an attempt to improve the therapeutic outcome and develop its skin-friendly formulation, we prepared the vesicle-based drug delivery system with capsaicin.

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An Ultra-High Pressure Liquid Chromatography Time-of-Flight Mass Spectrometry (UHPLC-TOF-MS) method for quantitative analysis of 30 drugs in whole blood was developed and validated. The method was used for screening and quantification of common drugs and drugs of abuse in whole blood received from autopsy cases and living persons.

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A reliable, fast, sensitive and selective Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS) method has been developed and validated for the determination of fenofibrate in marketed product (Lipanthyl) and human plasma. The chromatographic separation was performed on a reversed-phase Acquity®BEH C18 column (1.7 μm particle size, 50 mm x 2.1 mm ID) with an isocratic elution profile and mobile phase consisting of methanol and water (80:20, %, v/v). To achieve optimum chromatographic condition the influence of mobile phase composition and flow rate was investigated. The total chromatographic analysis time was as short as 2 min.

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