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Articles Related to validation

Validation of an Anti-Protective Antigen ELISA for Quantitative IgG Evaluation in B. anthracis Immunized Horses

The potency test for anthrax vaccines has historically involved the challenge of actively or passively immunized laboratory animals with a fully virulent strain of Bacillus anthracis. Lethal challenge studies with the archetypal virulent strains such as B. anthracis Ames strain present considerable difficulties in laboratory management and handling and are too inefficient for the early evaluation of alternative preventative and therapeutic interventions. An ELISA for the evaluation of antibody response to protective antigen (PA) in horses immunized with the Sterne 34F2 strain spore vaccine was developed. The objective of this work was to study the performance of this assay in terms of the guidelines set forth by the International Conference on Harmonics (ICH) and the Center for Biologics Evaluation and Research (CBER) for analytical procedures. We have demonstrated a working range for this assay (73-1581 EU/ml) on the bases of the following parameters: linearity (25 and 1,662 EU/ml, r2 = 0.9988, p < 0.001), accuracy (94.8 - 105.4 %, recovery within the range of 25 and 1,662 EU/ml), precision (≤ 17.6 % CV, repeatability; ≤ 15.7 and ≤ 13.1 % CV, intermediate precision per day and per analyst, respectively), limit of detection (2.25 EU/ml) and limit of quantitation (25 EU/ml). The assay was also demonstrated to be specific for the evaluation of anti-PA IgG antibodies. Based on the assay performance characteristics it was determined that the assay was adequate for use in B. anthracis immunogenicity testing in horses.
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Bioanalytical Method Development and Validation of Ferrum in Rabbit Plasma Samples by Derivatised Reverse Phase Liquid Chromatography and Application to Pharmacokinetic Study

Iron is an important component of hemoglobin, in addition to other positive roles like proper growth and development of humans and for growing of fetus in pregnancy women. Iron deficiency anemia is more prevalent among vegetarian than non vegetarians.
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Development and Validation of Knowledge, Attitude and Practice on Healthy Lifestyle Questionnaire (KAP-HLQ) for Malaysian Adolescents

To develop and determine validity and reliability of the Knowledge, Attitude and Practice on Healthy Lifestyle Questionnaire (KAP-HLQ) among lower secondary adolescents.
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Development and Validation of a Skin Fold Thickness Prediction Equation for Asian Indians Using Hydrodensitometry

Skin fold thickness prediction equations are widely used for estimating body fat percentage and thereby obesity. Equations to estimate body fat percentage for the Asian Indian population do not yet exist. The main purpose of this study was to develop and validate a new prediction equation for the estimation of body fat percentage in Asian Indian adults using anthropometric measurements (skin folds, circumference, bone breadth) and DEXA.
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The Medico-Legal Importance of Establishing Human Identity by Palatal Rugoscopy: Evaluation of the Immutability and Individuality of Palatal Rugae under the Influence of Ante Mortem Orthodontic Treatment

The palatal rugae can be an alternative method of forensic identification. Through the years, several investigations focused on the effect of orthodontic treatment in the palatal rugae pattern.
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Development and Validation of UV Spectroscopic First Derivative Method for Simultaneous Estimation of Dapagliflozin and Metformin Hydrochloride in Synthetic Mixture

The Novel, simple, sensitive, rapid, accurate and economical and reliable First derivative spectroscopic method has been developed for synthetic mixture of Dapagliflozin (DAPA) and Metformin hydrochloride (MET).
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Relative Bioavailability of Ciprofloxacin Doses (750 and 1000) mg in Healthy Male Volunteers by Using HPLC Method

A new, stable and accurate high performance liquid chromatography ultraviolet spectrophotometric method for quantitation of Ciprofloxacin in human plasma was developed and validated.
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Quantitative Analysis of 30 Drugs in Whole Blood by SPE and UHPLC-TOFMS

An Ultra-High Pressure Liquid Chromatography Time-of-Flight Mass Spectrometry (UHPLC-TOF-MS) method for quantitative analysis of 30 drugs in whole blood was developed and validated. The method was used for screening and quantification of common drugs and drugs of abuse in whole blood received from autopsy cases and living persons.
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Editorial Board Members Related to validation

Gillian Tully

Director
Principal Forensic Services Ltd
United Kingdom

Farzad Amirabdollahian

Principal Investigator
Department of Health Sciences
Liverpool Hope University
United Kingdom

Frank C. Barone

Professor
Department of Neurology
SUNY Downstate Medical Center
United States

Kristian Linnet

Professor
Department of Forensic Medicine
University of Copenhagen
Denmark

Jill Slay

Professor
Division of Information Technology, Engineering and the Environment
University of South Australia
Australia

NIKOLAS P. LEMOS

Professor
Pathology & Laboratory Medicine
University of California
United States

Alfonso Caracuel

Assistant Professor
Department of Psychology and Education
University of Granada
Spain

Osman Balci

Professor
Department of Computer Science
Virginia Polytechnic Institute and State University
United States

Yi Qian

Professor
Biomechanics
Australian School of Advanced Medicine
Macquarie University
Australia

Wael Ahmad Abu Dayyih

Associate Professor
Department of Pharmaceutical Medicinal Chemistry and Pharmaconosy
University of petra
Jordan
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