Articles Related to Biofilm

Biofilms are complex sessile microbial community extremely resistant to antibiotics. They typically form on both biotic and abiotic surfaces and are usually associated with high rates of mortality and morbidity in patients in hospital settings. Nosocomial infections are the major cause of infections seen worldwide. Bacterial Urinary Tract Infections (UTIs) represent the most common type of nosocomial infection that affect different parts of the urinary tract in both males and females.

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The virulent Escherichia coli strains are responsible for extraintestinal infections. However, no past studies have been undertaken via the presence of virulence genes, ability of biofilms formation and the frequency of Escherichia coli pathovars recovered from different butcheries and slaughterhouses in Tunisia. The aims of this study was to investigate 1-) the prevalence of pathogenic E. coli strains isolated from bovine, ovine, and poultry meat in Tunisia, 2-) to determinate the antibiotic resistance profiles, and 3-) to determine their biofilm-forming ability by three phenotypic methods .Totally, 36 E. coli isolates from meat of healthy animals (bovines, ovine, and chickens) collected from different butcheries and slaughterhouses were investigated by searching by PCR genes encoding virulence factors (hlyA, stx1, stx2, fimH, papC, eaeAet papG allèle III, ibeA, iutA, and antibiotic susceptibility testing. Biofilm production was detected by three phenotypic: Congo Red Agar (CRA) method, tube method (TM) and tissue culture plate (TCP) method. In addition, the genetic relationship of isolates was determined by PFGE.

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One of the challenges and concerns of water industry experts and practitioners is to ensure that there is no bacterial contamination in water samples, especially drinking water samples. The second is to find out if there is a microbial biofilm layer in water resources such as wells, storage tanks, and even water distribution networks. The presence of biofilms in water resources and reservoirs reduces the quality of water.

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Biofilm is the layer which is formed with the matrix produced through accumulation of the free organisms on a proper surface. This layer makes treatment of S.aureus infections difficult as it makes S.aureus resistant to antibiotics and inhibits phagocytosis.

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The pathogenity of S. aureus strains are related with features like its adherence, various toxins, enzymes, structural and extracellular factors. In our study, the relationship between biofilm formation and lipase, protease, urease activity were investigated in S. aureus strains isolated from various clinical specimens sent to our microbiology laboratory.

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I present some key considerations of the biofilm disease as part of two complex pathologies such as bone and joint infections and prosthetic joint infections, taking into account the bacterial pathogenic factors to understand the particular nature of these infections, and to achieve an accurate diagnosis and management beyond the antimicrobial therapy. I mention some personal experience of many years in the medical microbiology laboratory and next to the patient’s bed.

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Streptococcus mutans is a leading cariogenic pathogen of dental caries worldwide. Clinically, eliminating S. mutans from dental biofilms using antibiotics is not practical, because these agents indiscriminately kill other members of the resident flora, leading to ecological disruption and other negative clinical consequences. To develop target-specific antimicrobials, we evaluated several fusion peptides and identified a new peptide HP30 that showed a high selectivity for targeted killing of S. mutans.

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Biofilm is a common mode of fungal growth in clinical infection and Candida albicans is one of the species that are the most frequently associated with biofilm infection which has a significant impact on morbidity and mortality. In the mode of biofilm, C. albicans tends to display high resistance to body immunity and antimicrobial agents.

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