Articles Related to Chromatography
Activated Charcoal and Derivate Materials in Drugs and Biopharmaceutical Purification: Impurity Aspects
In literature are reported various use of activated charcoal AC and derivates in biopharmaceutical purifications.Aim of this
work is to verify impurity proficle when using this technology.Various commercial products are reported here but it is not
the scope of this work put in relation with any toxicological reaction: only to describe the technique used in this field.Because
various drugs and bioproduct need purification steps it is if of interest to see some material science peculiarity
Reversed-Phase High Performance Liquid Chromatography Procedure for the Determination of Isavuconazole in Human Plasma
Introduction: Isavuconazole is an antifungal drug used for treating patients with invasive fungal infections. Efficacy and safety of isavuconazole is monitored by measuring plasma isavuconazole concentration using LCMS which is a non-affordable method. We used the HPLC system with a UV detector to measure plasma Isavuconazole concentration.
Objective: Improved Reversed-phase high performance liquid chromatography procedure with UV detection is described which is cost effective, simple, precise and easily processed for the measurement of Isavuconazole, a drug used to treat the patients with invasive fungal infections, in blood plasma. Method: The method involves protein precipitation, addition of ammonium dihydrogen phosphate and chromatographic separation on a Hypurity C18 Column using an isocratic mobile phase of acetonitrile and ammonium acetate buffer (pH 8.0, 10 mM) (55:45, v/v). The UV detection was performed at 285 nm. The method provides rapid resolution of Isavuconazole in a 50 uL injection.
Result: Lower limit of Quantification (LLOQ) is 0.25 μg/ml in a 50 uL injection volume for Isavuconazole with a recovery consistently > 100 %. The assay is validated over linear range of 0.25 to 10 μg/ml. The intra-assay precision is < 3.53 % and inter-assay is <6.38% relative standard deviation of Isavuconazole. The method demonstrated clean separation, clinically acceptable detection limit and a linear range upto 25 ug/mL.
Conclusion: The assay demonstrated applicability in quantifying the drug level and monitoring the therapeutic dose for maintaining effective biological level to have better response in fungal infected patients. The method is cheaper as compared to LC-MS/MS and Tandem Mass spectrometry and the results are reportable on the same day of blood collection.
Exhaled Volatile Organic Compounds in Patients with Colonic Polyps
Background: Exhaled Volatile Organic Compounds (E-VOCs) may help to early detection of colonic polyps
Objective: Assess whether a new ambulatory device is able to detect specific E-VOCs in patients recently diagnosed with colonic polyps.
Material and Methods: All data were collected during routine consultations for Small Intestinal Bowel Overgrowth. A breath test was performed by X-PID 9500®.
Results: 203 patients older than 45 years of age were included. 31patients (15.3%) reported colonic polyps. 74 patients presented with gastroduodenal or jejunal (GDJ) liquid at ultrasound examination despite fasting.
96 patients (47.3%) exhaled numerous E-VOCs with a retention time between 12 to 45s (E-VOCs 12 to 45s). All patients of the polyp group exhaled E-VOCs 12 to 45s.
69 patients with GDJ liquid exhaled E-VOCS 12 to 45s. 11 patients out of these 69 cases (15.9%) present with polyps. 27 patients exhaled E-VOCs 12 to 45s and did not have GDJ, 20 of whom (74.1%) presented with polyps.
When GDJ liquid is not objectivized after fasting, the sensitivity of the E-VOCs 12 to 45s to identify patients with colonic polyps is equal to 100.0% and the specificity is 93.6%. The positive predictive value is 74.1% and the negative predictive value is 100%.
Conclusion: After fasting, X-PID 9500® is able to detect E-VOCs associated with colonic polyps, when GDJ liquid is excluded.
A Review Study on Adulteration in Bakery Goods
Adulteration in packed bakery products is done by making the product impure or altering its original form by adding materials or elements that are not usually part of it, especially inferior ones. It is finished by making the item debased or modifying its unique structure by adding materials or components that are not as a rule part of it, particularly mediocre ones [1]. It very well may be done deliberately by eliminating substances from food or inadvertently because of obliviousness, imprudence or absence of offices for keeping up with the nature of food. Potassium bromate is a flour improver that goes probably as a creating trained professional. Potassium bromates changes over into potassium bromide at high temperature during the baking framework [3]. Regardless, in case an abundance of is added, then again accepting the bread isn’t warmed long enough or not at an adequately high temperature, then, an extra total stay in the cake kitchen things, which may be dangerous at whatever point gobbled up [2]. It can cause stomach torture, free entrails, nausea, hurling, kidney dissatisfaction, oligonuria, anuria, deafness, dazedness, and hypotension, Detection is finished by spectrophotometric technique, IC/Mass spectrometry gives identification, Ion Chromatography (IC), High Performance Liquid Chromatography (HPLC) Synthetic tones in prepared merchandise Blue. #1 (Brilliant Blue) caused kidney growths in mice. Red.#3 (Erythrosine) Recognized as a thyroid cancer-causing agent in creatures. Red.#40 (Allura Red) speeds up the presence of invulnerable framework cancers in mice. It additionally causes extreme touchiness (sensitivity like) responses in certain purchasers and might trigger hyperactivity in youngsters. Yellow. #5 (Tartrazine) causes serious excessive responses. Yellow. #6 (Sunset Yellow) Caused adrenal growths in creatures and sporadically causes extreme responses. Distinguishing proof of engineered food colors corruption by paper chromatography and spectrophotometric strategies. In Adulterated chocolate two defilements are utilized arrowroot and dim rye flour distinguished by Near-Infrared (NIR) spectroscopy [14].
Exhaled Volatile Organic Compounds and Vagal Tone are Different in Patients with Overweight or with Obesity: Practical Consequencies
Urinary Volatile Organic Compounds have been associated with type 2 diabetes mellitus and overweight and may
correlate with Exhaled Volatile Organic Compounds (E-VOCs).
Exhaled Volatile Organic Compounds in Patients with a Medical History of Cancer/Dysplasia
Exhaled Volatile Organic Compounds (E-VOCs) have been associated with cancer and may help to early detection or
even identify pre-cancerous status.
A Simple Method for the Quantification of Atenolol from the Forensic Human Blood and Visceral Samples by High Performance Thin Layer Chromatography
A simple High-Performance Thin layer chromatography (HPTLC) method has been developed for determination of atenolol in human
blood and viscera. The mobile phase was ethyl acetate, acetone, ethyl alcohol, ammonia solution in the ratio of 45:45:7:3. The densitometry
scanning at 254 nm was found to be maximum absorption for standard atenolol. The standard and extracted atenolol from samples were
detected by HPTLC and confirmed with authenticated spectra at 254 nm. The quantity of the atenolol determined to be 1.6 µg for viscera
and 1.5 µg for blood sample.
A Study on Chromatography Methods for the Separation and Detection of Certain Benzodiazepine Drug in Forensic Sample
Forensic Scientists are required to identify an ever increasing and more complex assortment of drugs and related compounds.
Bioequivalence between two extended Release Tablets of Oxycodone Hydrochloride in Healthy Subjects under Fasting and Fed Conditions
Bioavailability of different formulations of oxycodone 20 mg extended release tablets was compared in two bioequivalence studies, one under fasting conditions and the other one after a high-fat breakfast.
Bioequivalence between two Prolonged Release Tablets of Desvenlafaxine Succinate in Healthy Subjects under Fasting and Fed Conditions
Bioavailability in different formulations of desvenlafaxine100 mg prolonged-release tablets was compared in two bioequivalence studies, one under fasting conditions and the other after a standard breakfast. Both studies were a single dose, randomized, open-label, two-period crossover, with Brazilian male and female healthy subjects. Blood samples were taken during 48 h and plasmatic concentrations were determined using a validated UPLC-MS/MS method.
Estimation of Beta-Lactam Antibiotics-Serum Albumin Conjugated Proteins by High Performance Liquid Chromatography with Ultraviolet Detector
β-lactam antibiotics represented the oldest class of antibiotics used in the treatment of infections in a human being, livestock, and aquaculture. Drugs residues in the edible tissues or foodstuffs of animal pose a potential threat to human health. At present, extensive antibiotic-resistant strains are now being detected and the development of antibiotic-resistant strains of epidemic and zoonotic pathogens is a major concern. Therefore, the purpose of this study was to estimate the extent of β-lactam antibiotics-HSA/BSA conjugations via three conjugation methods by using high-performance liquid chromatography (HPLC) with ultraviolet (UV) detector. We wish that the technique will be applied to preliminarily detect the residues of β-lactam antibiotics may persist in edible tissues or foodstuffs in the livestock and aquaculture farms in clinical.
Multidimensional LC/MS-MS Analysis of Synthetic Cannabinoids in Urine, Plasma, and Edibles
Synthetic cannabinoids (SCs) present a multitude of problems in terms of maintaining up-to-date methods of detection. They are novel psychoactive substances originally synthesized for medical use and research purposes. Abuse has demonstrated a variety of effects ranging from euphoria to aggressive behavior and death. The marketing, similar naming, and described pharmacological interactions create the dangerous and false perception that SCs are similar to, or the same as, tetrahydrocannabinol in cannabis products
Novel analytical technologies of Quality in the Sheep & Goat Dairy Sector
France, Greece, Italy, and Spain the major sheep and goat milk producers in Europe, got the initiative through an interim of 30 years (1985 – 2015), to organize - through IDF - 7 symposiums aiming at the upgrade of the Dairy Sector. One major interest was the milk quality. Stepwise, the efforts followed cow milk structural development in organizing the testing laboratory, the definition of quality in composition (fat, protein, lactose, total solids), in Hygienic (Total Bacteria Count, Somatic Cell Count, antibiotics residues and Aflatoxin M1 contamination), adulteration with water and detection of milk species. EU, by Decision 2002/657/EC, defined the rules for the Directive 96/23/EC, enumerated the testing methods, the demands in sampling procedures, the minimum limits of performance, the quality control (ISO 17025) and the interpretation of results. The equivalency of results from different laboratories, through ISO 17025, demanded the application of ISO analytical standards.
Bioanalytical Method Development and Validation of Ferrum in Rabbit Plasma Samples by Derivatised Reverse Phase Liquid Chromatography and Application to Pharmacokinetic Study
Iron is an important component of hemoglobin, in addition to other positive roles like proper growth and development of humans and for growing of fetus in pregnancy women. Iron deficiency anemia is more prevalent among vegetarian than non vegetarians.
Editorial Board Members Related to Chromatography
Loai Aljerf
Professor
Department of Basic Sciences
Faculty of Dental Medicine
Damascus University
Syria
Department of Basic Sciences
Faculty of Dental Medicine
Damascus University
Syria
