Articles Related to cell

The aim of controlling/regulating cellular processes by surface patterning of biomaterials is a crucial concept in emerging research fields, where the precise fabrication of micro-nano surface topographies is required at manufacturing scales to meet consumer demand. Photolithography is typically employed for surface patterning; however, the high cost, process complexity, and lack of environmentally friendly and sustainable methodologies represent steep barriers for many researchers. Soft lithographic methods have been utilised extensively as a potential alternative for traditional photolithography, yet a photolithographic step is still required for initial template fabrication. Here, we demonstrate the use of repurposed optical consumer items such as DVDs, diffraction glasses, and gratings that would otherwise be thrown away after their useful lives, being used here as a sustainable approach as templates for the formation of complex nano-patterns. By replicating these templates in polydimethylsiloxane (PDMS) and polylactic acid (PLA), we show the varied interactions of human aortic smooth muscle cells (HAoSMC) to these surfaces. Notably, it was demonstrated that, in comparison to nonpatterned substrates, HAoSMC interaction (adhesion, spreading, and viability) was higher on DVD patterned surfaces compared to diffraction glass and grating patterned surfaces according to the results from cell metabolic activity (MTT and Alamar Blue), and proliferation (total DNA) assays. We demonstrate that a simple, reusable, inexpensive, yet robust technology could be a powerful tool in improving our understanding of cell-surface interactions and for the potential development of medical devices with micro- and nanoscale low or high adhesion features

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Intravascular large B-cell lymphoma (IVBCL) is a rare but aggressive form of non-Hodgkin lymphoma. It is characterized by the malignant proliferation of lymphocytic tumor cells in the lumens of capillaries, small arterioles, and post-capillary venules. There exists two clinical variants: the Asian variant and the Western variant. The former is associated with neurologic and dermatologic signs and symptoms, whereas the latter often presents with splenomegaly, jaundice, and hemophagocytes. Lymphadenopathy is uncommon, and patients typically have nonspecific symptom

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The relationship between blood groups and diseases has been a subject of interest for many researchers. This study aims to investigate the susceptibility of each blood group to anemia based on the relationship between the erythrocyte indices and the biochemical parameters used to diagnose anemia.

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Microcrystalline cellulose (MCC) is a key excipient in formulation and production of pharmaceutical solid dosage forms such as tablets. In this study, the microcrystalline cellulose (Novicel™) obtained from sugarcane bagasse (a waste material from sugarcane factories) was compared with Avicel PH 101 (a commercially available MCC) for its physicochemical characteristics and potential as a dry binder in direct compression of ascorbic acid tablets.

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The BDNF/TrkB signaling is a robust regulatory feature of neuronal cells that promotes survival, differentiation, and proliferation in a variety of cell types and animal models for neurodegenerative diseases. The activation of TrkB by BDNF in clinical trials is accompanied by remarkable side effects and appears to be challenging due to BDNF's adverse molecular profile

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Background: Despite their good efficacy, antiretroviral drugs often induce adverse effects, in particular hypersensitivity reactions (HSR) which cause significant discomfort leading to the abandonment of treatment and then to therapeutic failure in some HIV-positive patients. Therefore, in this study, we aimed to assess the association between immune responses and hypersensitivity reactions (HSR) induced by antiretroviral treatment in people who living with HIV-1 (PLHIV-1), through the distribution of immune cells and cytokine profiles.

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Intracranial hemorrhage (ICH) is a life-threatening complication in hemophilia. It often occurs during childhood after minimal head trauma. It poses a problem of diagnostic and therapeutic management, especially in Africa where CFCs are not always available.

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Penicillin and streptomycin are the most commonly used to prevent bacterial infection or contamination in cell culture. However, the role of Penicillin-streptomycin on inducing mouse embryonic stem cells(mESCs) to differentiate into cardiomyocytes is unclear. To illustrate the mechanism of penicillin-streptomycin on inducing mESCs differentiation into cardiomyocytes, we firstly investigated mESCs differentiation into cardiomyocytes by the traditional method. The time- space expressions of cardiac-specific genes were detected by real-time PCR and western blotting at 8 different time-points over a period of 36 days. Furthermore, action potentials were measured by patch clamp for mESC-derived cardiomyocytes. Embryonic bodies through hanging drop method, were incubated with penicillin-streptomycin (P/S) at different concentrations (1×PBS, 1×, 2×, 5×), and then, the number of cardiomyocytes and expression levels of myocardial marker proteins were detected by flow cytometry, cellular immunofluorescence, and western blotting, respectively. We found that the SSEA-1 of mESC presented green fluorescence, and H.E. staining showed karyoplasmic ratio >>1. Besides, TNNI3, TNNT2, ACTN2 and MYL-2v were clearly visible by cellular immunofluorescence. Furthermore, the ACTN2 positive cells rate of 2× and 5× groups were remarkably higher than that in Cntl (1×PBS) group and 1× group (10 Units/mL penicillin; 10 μg/mL streptomycin), as well as the expressions of GATA4, MYL-2v, TNNI3, ACTN2

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Carica papaya x vasconcelleacauliflora and intergeneric F1 hybrids of these species were screened for resistance to severely infected papaya ringspot virus isolates of papaya ringspot virus. Artificial screening for papaya ringspot virus was carried out 27 days after sap inoculation. Out of twenty-nine F1 hybrid plants of CO 7 x Vasconcelleacauliflora, only six plants were found free from PRSV symptoms. Similarly, out of fifty-five F1 hybrid plants of PusaNanha x Vasconcelleacaulifloraonly twenty-three were found free from the symptoms and seventy plants out of 335 plants of CP50 x Vasconcelleacauliflora were found free from PRSV symptoms. The resistance of the hybrids and parents and their hybrids viz, CO 7 x Vasconcelleacauliflora, PusaNanha x Vasconcelleacauliflora and CP50 x Vasconcelleacauliflora were subjected to DAS ELISA test. Molecular marker viz, ISSR markers were used to check and verify the hybridity. ISSR markers showed confirmity on three hybrid progenies viz, CO7V3, CO7V5 and CO7V6 from CO 7 xVasconcelleacauliflora.

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Abstract Background: Clinical studies demonstrated the efficacy of therapies based on the autologous grafting of adult mesenchymal stem cells to accelerate the healing and regenerative processes of the skin and mesenchymal tissues therefore, this is considered a valuable approach in the aesthetic rejuvenation treatment to give volume restoration and skin regeneration effects. Objective: The aim of this project consists of the evaluation of the cell viability of adipose tissue (AT) harvested using the Superficial Enhanced Fluid Fat Injection (SEFFI) procedure standardized by the Authors (AG, FPB). The harvesting procedure was performed using two different cannulas having 0.8 mm and 1 mm side-port holes, respectively. Cells phenotype and ability to adhere to the plastic surface have been analysed. The results have been compared to those recorded in adipose tissue harvested using a liposuction system and processed with enzymatic digestion (collagenase). Methods: This study was performed on adipose tissues harvested from 7 patients (6 females and 1 male) with an average age of 48.5 years with two different techniques and three different cannulas. We compared the cell vitality of every sample at T0 and T72. Moreover, the samples were analysed to determine the phenotype: cells were incubated with antibodies anti human against CD90-FITC, CD73-PeCy7, CD44-PE, CD31-PECy5, CD235a- PECy7, CD34-FITC, CD45-FITC and CD146-FITC and read using the flow cytometer s3e Cell Sorter, BioRad. Results were analysed using the software Flow Jo.

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Purpose: We aimed to create mechanic optic nerve injury model in rats and investigate the neuroprotective effects of ripasudil on retinal ganglion cells. Study Design: Experimental study Methods: Mechanic optic nerve injury model was created in the right eyes of male Wistar rats (n=15). Rats were divided into three groups: glaucoma model with sham treatment (group1) and 20 µM intravitreal ripasudil treatment (group 2) and 50 µM intravitreal ripasudil treatment (group 3). Treatment was applied intravitreally and rats were sacrificed at the end of 4 weeks. Glial fibrillary acidic protein (GFAP), Brn-3a antibody,anti- Iba1 was examined by immunohistochemistry. Results: The number of Brn-3a positive RGC in the mechanical optic nerve injury model was 5.33 ± 2.08 (min: 3, max: 7) in sham group, 10.25 ± 2.63 (min: 8, max: 14) in 20 µM group and 16.75 ± 5.43 (min:9, max: 21) in 50 µM group (p <0.05). GFAP positive RGC counts were recorded as 24.33 ± 2.08 (min: 22, max: 26) in sham group, 16.75 ± 1.70 (min: 15, max: 19) in 20 µM group and 13.00 ± 4.08 (min:10 , max: 19) in 50 µM group (p <0.05). Ripasudil treatment also decreased Iba1 expression in the retina of mechanic optic nerve injury groups. In addition, ripasudil treatment prevented apoptotic cell death by increasing Bcl-xL protein expression and preserved Tfam protein expression in the retina. Conclusions: Our experimental study has shown that ripasudil is neuropreotective in mechanical optic nerve injury model.

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Bloodstream infections (BSI) are one of severe infectious complications faced by hematopoietic stem cell transplantation (HSCT) patients. BSI increases significantly the morbidity and mortality of HSCT patients. Gram-positive bacteria occurred more frequency than gram-negative bacteria over past decades, but rates of gram-negative bloodstream infections have recently increased again. Antibacterial prophylaxis could be justified in HSCT, infections caused by resistant pathogens increased mortality. New antibiotics such as omadacycline, meropenem/vaborbactam, eravacycline, ceftobiprole, tedizolid, dalbavancin, cefiderocol hold promise for the treatment of highly resistant pathogens.

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Introduction: Sickle cell disease (SCD) is a genetic hemoglobin disease for which there are many treatment options. Evidence supports the efficacy and cost-effectiveness of hydroxyurea (HU). Nevertheless, its use and adherence to treatment remain suboptimal, hence, the necessity to assess the factors influencing this observance. Objectives: The objective was to evaluate the factors influencing observance to HU in children with sickle cell disease from the Mother and Child Center of the Chantal Biya Foundation (MCC of the CBF). Materials and Methods: This was a descriptive and analytical cross-sectional study from January to June 2020. Children on HU for at least six months, and followed up at MCC of the CBF were included. Data collection was done using a pre-tested questionnaire. The level of compliance was assessed using the Morisky’s observance grid, where 8 points was considered as “good”; 6-7 points as “fair”, and <6 as “poor”. Data were stored in CSPro version 7.0 software, and subsequently analyzed using the SPSS version 20.0 software. Results: Of 142 patients recruited, the level of patient observance was considered “good” in 2.1%, fair in 26.1% and poor in 71.8%. Logistic regression analyses suggested that using hydroxyurea to prevent complications was the only factor that favored good medical adherence [OR=0.37; CI (0.14-0.987)] (p=0.047). Poor compliance was mainly associated with the fear of infertility and and other sides effects associated with the use of HU. Conclusion: Children with SCD in this study have poor compliance with HU. Implementing IEC sessions would make it possible to raise patient/parent awareness and reverse this trend.

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The application of electronic-nose (E-nose) technologies in forensic science is a recent new development following a long history of progress in the development of diverse applications in the related biomedical and pharmaceutical fields. Data from forensic analyses must satisfy the needs and requirements of both the scientific and legal communities. The type of data collected from electronic-nose devices provides a means of identifying specific types of information about the chemical nature of evidentiary objects and samples under investigation using aroma signature profiles of complex gaseous mixtures containing volatile organic compounds (VOCs) released from manufactured products and parts of the human body. E-nose analyses also provide useful qualitative information about the physicochemical characteristics and metabolic conditions of human subjects without the need for time-consuming analyses to identify all chemical components in human-derived volatile mixtures.

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The collection of human odor volatiles is of interest to forensic applications as a path to investigate canine scent discriminations in legal investigations. A study using a selected array of previously identified human odor compounds has been conducted to determine the retention and release capabilities of five (5) natural and synthetic fabric types, cotton (mercerized fabric and gauze matrix), polyester, rayon and wool.

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The design of an electrochemical reactor for the preparation of self-supported comparatively thin (up to 10 μm) and large area (up to 50 cm2) anodic alumina membranes is described allowing growth of porous alumina at high applied potential (up to 150 V) without burning.

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Vertical Cu2ZnSnS4 (CZTS) nanowire arrays have been synthesized via two-step electroplating method into anodized aluminum oxide template. For deposition of CZTS nanowires, anodized aluminum oxide (AAO) was used as the growth mask for the growth of the nanowires. AAO templates with hole sizes of 70 nm in diameter were used in the experiments.

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