Articles Related to gene expression

Gene Therapy in Sickle Cell Disease

Sickle cell disease is a prevalent and severe monogenic disorder resulting from a homozygous missense mutation in the β-globin gene that leads to polymerization of hemoglobin S. Clinical manifestations of the disease can be critical with considerable morbidity and mortality. One treatment option for the disease is bone marrow transplantation. However this method is restricted to the patients with an appropriately matched donor. Gene therapy by either gene insertion or gene editing, utilizing patient’s own cell is a primary therapeutic option to cure sickle cell disease. However, very less clinical trials have been performed with genetic therapy for treating Sickle cell disease (SCD). Since a couple of decades significant progress has been made in the area of gene therapy for treating monogenic hemoglobin disorders. Numerous therapies are currently in clinical trial stages or in preclinical stages. The safety and efficacy of gene therapy has been greatly improved with the initial use of γ-retrovirus vectors, followed by next-generation lentivirus vectors, and latest gene editing techniques. Although the clinical interpretation of gene therapy has been successful, it involves some limitations including complex cellular abnormalities, inadequate transgene expression, and challenges in achieving effective and persistent inhibition of polymerization of hemoglobin S. This review intends to discuss gene therapy strategies specific to Sickle cell disease, present state of the field, and current status of the gene therapy clinical trials.

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DNA Methylation Status of the Methylenetetrahydrofolate Reductase Gene is associated with Depressive Symptoms in Japanese Workers: A Cross-Sectional Study

Polymorphisms in methylenetetrahydrofolate reductase (MTHFR) gene, that is considered to be the most important genetic determinant of blood homocysteine concentration, are associated with various diseases, including psychiatric disorders. However, the epigenetic factors influencing on the transcription and expression of this gene are unclear.

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Human Induced Pluripotent Stem Cells Derived from A Patient with Sporadic Alzheimer’s Disease Exhibit Altered Neuronal Proliferative Capacity

We generated two hiPSC clones from a patient with sporadic late-onset Alzheimer’s disease (AD-iPSCs), which expressed typical undifferentiated markers and passed standard pluripotency assays. Genome-wide microarray analysis revealed that AD-iPSCs were highly similar to control hiPSCs and hESCs, albeit with some noticeable differences in few genes, viz.: DNAJC15, GRPR, NAIP and SNORD116-18

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Fangchinoline Inhibits Breast Tumor Proliferation and Induces Apoptosis in MDA-MB-231 Cell Line in Vivo

Immunohistochemistry (IHC) was used to determine the protein levels of p27, CD117 and ki67 in tumor tissue of xenograft. After treatment of Fan, the morphologic changes were observed by DAPI staining, Hoechst staining and TUNEL detection under fluorescent microscopy.

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Modeling of Gene Regulatory Networks: A Literature Review

In the last years numerous methods have been developed and applied to reconstruct the structure and dynamic rules of gene-regulatory networks from different high-throughput data sources such as gene expression data.

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